Results 11 to 20 of about 115,445 (261)

Two-dimensional electrophoresis protocols

open access: yesEuropean Journal of Histochemistry, 2010
It is worthy to buy this series of protocols on a classical well established technique, especially for those are newcomer users: I say this because today quite often the youngest colleagues are totally devoted to smashing techniques forgetting those ...
CarloAlberto Redi
doaj   +1 more source

A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS

open access: yesMethodsX, 2017
A method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied. After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip
Stanislav Naryzhny   +4 more
doaj   +1 more source

Alpha1-acid glycoprotein post-translational modifications: a comparative two dimensional electrophoresis based analysis

open access: yesItalian Journal of Animal Science, 2010
Alpha1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma.
P. Roncada   +7 more
doaj   +1 more source

Proteomic Analysis of Polysaccharide-Milk Protein Interactions Induced by Chitosan

open access: yesMolecules, 2015
The chitosan-induced coacervation of milk proteins was investigated using a proteomic approach. The addition of 0.8% chitosan to milk caused the milk proteins to coacervate after a 1 h incubation period.
Chun-Chi Chen   +2 more
doaj   +1 more source

METHODOLOGICAL ASPECTS OF IDENTIFICATION OF TISSUE-SPECIFIC PROTEINS AND PEPTIDES FORMING THE CORRECTIVE PROPERTIES OF INNOVATIVE MEAT PRODUCTS

open access: yesТеория и практика переработки мяса, 2018
One of the ways to address the food quality issues facing the industry is the development of standardized and certified methods related to the conduct of in-depth studies of biochemical indicators of quality and safety of meat and meat products.
Natal’ya L. Vostrikova   +2 more
doaj   +1 more source

A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]

open access: yesIranian Journal of Medical Physics, 2015
Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi   +3 more
doaj   +1 more source

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

open access: yesProteomes, 2016
The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy   +2 more
doaj   +1 more source

AN INFLUENCE OF SPONTANEOUS MICROFLORA OF FERMENTED HORSEMEAT PRODUCTS ON THE FORMATION OF BIOLOGICALLY ACTIVE PEPTIDES

open access: yesТеория и практика переработки мяса, 2018
At present, different methods are used to accumulate functional peptides in meat raw materials, including the use of spontaneous microflora during autolysis, the use of the microbial enzymes (the application of starter cultures) and the use of the non ...
I. M. Chernukha   +6 more
doaj   +1 more source

Comparative proteomic study of pig muscle proteins during growth and development of an animal

open access: yesТеория и практика переработки мяса, 2022
The production of high-quality pork is closely related to the growth and development of muscle tissue. The present article provides a comparative proteomic research of l. dorsi, b. femoris, m. brachiocephalicus during the pigs’ growth and development (at
A. G. Akhremko, E. S. Vetrova
doaj   +1 more source

Purification of 38kDa Antigen of Mycobacterium tuberculosis by Two Dimensional Preparative Electrophoresis

open access: yesBio-Protocol, 2012
Serodiagnosis of tuberculosis using purified native antigens is one of the approach for the early detection of TB. This protocol gives the details for growing Mycobacterium tuberculosis in liquid culture and one proven way to get a good yield of the ...
Uma Ranganathan
doaj   +1 more source

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