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Two-Dimensional Difference Gel Electrophoresis
Nature Protocols, 2006The two-dimensional (2D) polyacrylamide gel-based approach to protein profiling has been successful because it is an accessible, inexpensive, and powerful tool for the analysis of global patterns of protein expression. All protein spots that are resolved and detected within the 104 to 105 dynamic range of gel capacity can be studied qualitatively and ...
Surya, Viswanathan +2 more
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Two-dimensional electrophoresis computerized processing
International Journal of Biochemistry, 1988This paper describes various methods suitable for implementation of two-dimensional processing software. The different steps leading to a complete processing are described, from the digitalization of the image to the processing of the resulting data. The characteristics of a convenient digitalization system are discussed. The different software devoted
P, Vincens, P, Tarroux
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Two-dimensional strandness-dependent electrophoresis
Nature Protocols, 2006Two-dimensional strandness-dependent electrophoresis (2D-SDE) separates nucleic acids in complex samples according to strandness, conformation and length. Under the non-denaturing conditions of the first electrophoretic step, single-stranded DNA, double-stranded DNA and RNA.DNA hybrids of similar length migrate at different rates.
Gudmundur H, Gunnarsson +4 more
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Two-dimensional electrophoresis
Nature, 1991Recent developments in the first- and second-dimension steps of two-dimensional electrophoresis have improved pattern reproducibility.
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Two-Dimensional Difference Gel Electrophoresis
2018Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample are covalently tagged with different color fluorescent dyes that are designed to have no effect on the relative migration of proteins
Malachi, Blundon +4 more
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Two-Dimensional Electrophoresis
1997In organelles, cells and tissues of all organisms complex functions and metabolic reactions are maintained by proteins within and at the surface of each compartment. The number of proteins occurring in a biological compartment corresponds to the complexity of the functions it has to fulfill.
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Two-Dimensional Electrophoresis for Plant Proteomics
2006Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry (MS) is currently the workhorse for proteome analysis. 2-DE allows separation of highly complex mixtures of proteins according to isoelectric point (pI), molecular mass (Mr), solubility, and relative abundance and ...
Walter, Weiss, Angelika, Görg
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Alignment of two-dimensional electrophoresis gels
Biochemical and Biophysical Research Communications, 2007Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment.
Shi, Guihua +4 more
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Two-Dimensional Electrophoresis: An Overview
2009Two-dimensional gel electrophoresis (2DE) separates proteins by molecular charge and molecular size. Proteins are first solubilised in a denaturing buffer containing a neutral chaotrope, a zwitterionic or neutral detergent, and a reducing agent. First-dimension isoelectric keywords, focusing, then subjects proteins to a high voltage within a pH ...
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Two-dimensional gel electrophoresis of proteins
Journal of Chromatography B: Biomedical Sciences and Applications, 1987The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures. The evolution of two-dimensional electrophoresis is briefly described.
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