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High-Resolution Two-Dimensional Electrophoresis
2009Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry is currently the workhorse for the majority of ongoing proteome projects. Although alternative/complementary technologies, such as MudPIT, ICAT, or protein arrays, have emerged recently, there is up to now no ...
Walter, Weiss, Angelika, Görg
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Two-dimensional electrophoresis of membrane proteins
Analytical and Bioanalytical Chemistry, 2007One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation ...
Ralf J, Braun +3 more
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Glycoproteomic Analysis by Two-Dimensional Electrophoresis
2009Changes in N-linked glycosylation are known to occur during the development of cancer. For example, increased branching of oligosaccharides has been associated with metastasis and has been correlated to tumor progression in human cancers of the breast, colon, and melanomas.
Mary Ann, Comunale, Anand, Mehta
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Diversity of puroindolines as revealed by two‐dimensional electrophoresis
PROTEOMICS, 2003AbstractPuroindolines are endosperm lipid binding proteins, which are separated by reversed phase‐high‐performance liquid chromatography or cation exchange chromatography into two isoforms, puroindoline‐a (PIN‐a) and puroindoline‐b (PIN‐b). Being very basic and close in molecular weight, PIN‐a and PIN‐b have never been separated using conventional ...
Branlard, G. +6 more
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Two-dimensional electrophoresis image interpretation
IEEE Transactions on Biomedical Engineering, 1993A novel method to interpret two-dimensional electrophoresis gels is presented. Genetic background and electrophoretic processes are summarized. Present methods to analyze gel images and to exploit series of gels are described, then their drawbacks are outlined.
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Two‐dimensional electrophoresis
Neurology, 1984Carol S. Giometti, Morris J. Danon
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Two-Dimensional Difference Gel Electrophoresis
2009The introduction of two-dimensional fluorescent difference gel electrophoresis has enabled the extensive screening of differential protein expression levels with higher confidence and greater sensitivity than using the classical two-dimensional electrophoresis (2DE) approach.
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Two-Dimensional Difference Gel Electrophoresis
2012Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2DE) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample are covalently tagged with different color fluorescent dyes that are designed to have no effect on the relative migration of proteins ...
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Two-dimensional electrophoresis and ionophoresis
Journal of Colloid Science, 1951Abstract The author has reported recently a unidimensional microelectrophoretic technique in which an electrical potential is applied across the ends of strips of filter paper saturated with buffer or other electrolyte solutions. To these strips are applied at intermediate areas, mixtures of charged substances to be separated.
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Two‐dimensional electrophoresis on paper
Journal of Polymer Science, 1954AbstractWith a view to finding a better method of separating the different components during electrophoresis on paper, we have carried out the electrophoresis in two dimensions, first flowing the current in one direction and then at right angles to that direction.
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