A robust high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels [PDF]
The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects.
Harrington, Michael G. +1 more
core +1 more source
Proteomic Analysis of Polysaccharide-Milk Protein Interactions Induced by Chitosan
The chitosan-induced coacervation of milk proteins was investigated using a proteomic approach. The addition of 0.8% chitosan to milk caused the milk proteins to coacervate after a 1 h incubation period.
Chun-Chi Chen +2 more
doaj +1 more source
Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold +225 more
core +4 more sources
One of the ways to address the food quality issues facing the industry is the development of standardized and certified methods related to the conduct of in-depth studies of biochemical indicators of quality and safety of meat and meat products.
Natal’ya L. Vostrikova +2 more
doaj +1 more source
A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]
Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi +3 more
doaj +1 more source
Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C +3 more
core +2 more sources
Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis
The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
doaj +1 more source
At present, different methods are used to accumulate functional peptides in meat raw materials, including the use of spontaneous microflora during autolysis, the use of the microbial enzymes (the application of starter cultures) and the use of the non ...
I. M. Chernukha +6 more
doaj +1 more source
Comparative proteomic study of pig muscle proteins during growth and development of an animal
The production of high-quality pork is closely related to the growth and development of muscle tissue. The present article provides a comparative proteomic research of l. dorsi, b. femoris, m. brachiocephalicus during the pigs’ growth and development (at
A. G. Akhremko, E. S. Vetrova
doaj +1 more source
Serodiagnosis of tuberculosis using purified native antigens is one of the approach for the early detection of TB. This protocol gives the details for growing Mycobacterium tuberculosis in liquid culture and one proven way to get a good yield of the ...
Uma Ranganathan
doaj +1 more source

