Results 21 to 30 of about 244,648 (302)

A robust high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels [PDF]

open access: yes, 1993
The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects.
Harrington, Michael G.   +1 more
core   +1 more source

Proteomic Analysis of Polysaccharide-Milk Protein Interactions Induced by Chitosan

open access: yesMolecules, 2015
The chitosan-induced coacervation of milk proteins was investigated using a proteomic approach. The addition of 0.8% chitosan to milk caused the milk proteins to coacervate after a 1 h incubation period.
Chun-Chi Chen   +2 more
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yes, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold   +225 more
core   +4 more sources

METHODOLOGICAL ASPECTS OF IDENTIFICATION OF TISSUE-SPECIFIC PROTEINS AND PEPTIDES FORMING THE CORRECTIVE PROPERTIES OF INNOVATIVE MEAT PRODUCTS

open access: yesТеория и практика переработки мяса, 2018
One of the ways to address the food quality issues facing the industry is the development of standardized and certified methods related to the conduct of in-depth studies of biochemical indicators of quality and safety of meat and meat products.
Natal’ya L. Vostrikova   +2 more
doaj   +1 more source

A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]

open access: yesIranian Journal of Medical Physics, 2015
Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi   +3 more
doaj   +1 more source

Capabilities and limitations of gel electrophoresis for elemental speciation : a laboratory's experience [PDF]

open access: yes, 2006
Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper is based on four years of practical experience of the authors' lab in this domain
Chéry, Cyrille C   +3 more
core   +2 more sources

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

open access: yesProteomes, 2016
The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy   +2 more
doaj   +1 more source

AN INFLUENCE OF SPONTANEOUS MICROFLORA OF FERMENTED HORSEMEAT PRODUCTS ON THE FORMATION OF BIOLOGICALLY ACTIVE PEPTIDES

open access: yesТеория и практика переработки мяса, 2018
At present, different methods are used to accumulate functional peptides in meat raw materials, including the use of spontaneous microflora during autolysis, the use of the microbial enzymes (the application of starter cultures) and the use of the non ...
I. M. Chernukha   +6 more
doaj   +1 more source

Comparative proteomic study of pig muscle proteins during growth and development of an animal

open access: yesТеория и практика переработки мяса, 2022
The production of high-quality pork is closely related to the growth and development of muscle tissue. The present article provides a comparative proteomic research of l. dorsi, b. femoris, m. brachiocephalicus during the pigs’ growth and development (at
A. G. Akhremko, E. S. Vetrova
doaj   +1 more source

Purification of 38kDa Antigen of Mycobacterium tuberculosis by Two Dimensional Preparative Electrophoresis

open access: yesBio-Protocol, 2012
Serodiagnosis of tuberculosis using purified native antigens is one of the approach for the early detection of TB. This protocol gives the details for growing Mycobacterium tuberculosis in liquid culture and one proven way to get a good yield of the ...
Uma Ranganathan
doaj   +1 more source

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