Results 31 to 40 of about 244,648 (302)
Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]
, 2017 Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire +4 more sources
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source
Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients [PDF]
, 1982 In Neurospora, one protein associated with the mitochondrial small ribosomal subunit (S-5, Mr 52,000) is synthesized intramitochondrially and is assumed to be encoded by mtDNA.
Lambowitz, Alan M., LaPolla, Robert J.
core +1 more source
APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE
Теория и практика переработки мяса, 2018 Eight protein substances that undergo changes during autolysis were found using the electrophoretic methods with the following mass spectrometric identification.
Irina M. Chernukha +1 more
doaj +1 more source
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source
Proteomic evaluation of sheep serum proteins
BMC Veterinary Research, 2012 Background The applications of proteomic strategies to ovine medicine remain limited. The definition of serum proteome may be a good tool to identify useful protein biomarkers for recognising sub-clinical conditions and overt disease in sheep.
Chiaradia Elisabetta +7 more
doaj +1 more source
What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
Proteomes, 2020 Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus +2 more
doaj +1 more source
Advances in Cancer Biology - Metastasis, 2022 Objectives: Tobacco chewing and smoking practices have been a common trend among human populations. Tobacco addiction is a commonly known etiology for oral cancers.
Sapna Khowal +3 more
doaj +1 more source
Proteomics-on-a-chip for Biomarker discovery [PDF]
, 2007 In proteomics research still two-dimensional gel electrophoresis (2D-GE) is currently used for biomarker discovery. We applied free flow electrophoresis (FFE) separation technology combined with biomolecular interaction sensing using Surface Plasmon ...
Berg, Albert van den +4 more
core +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source