Results 31 to 40 of about 179,081 (177)

Evidence for a Xer/dif system for chromosome resolution in archaea. [PDF]

open access: yesPLoS Genetics, 2010
Homologous recombination events between circular chromosomes, occurring during or after replication, can generate dimers that need to be converted to monomers prior to their segregation at cell division.
Diego Cortez   +6 more
doaj   +1 more source

Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis

open access: yesFrontiers in Microbiology, 2021
Multiplex engineering at the scale of whole genomes has become increasingly important for synthetic biology and biotechnology applications. Although several methods have been reported for engineering microbe genomes, their use is limited by their complex
Aihua Deng   +12 more
doaj   +1 more source

Promoter Orientation of Prokaryotic Phase-variable Genes by PCR

open access: yesBio-Protocol, 2012
One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR.
Stacey Bateman, Patrick Seed
doaj   +1 more source

Tyrosine Recombinase Retrotransposons and Transposons

open access: yesMicrobiology Spectrum, 2015
ABSTRACT Retrotransposons carrying tyrosine recombinases (YR) are widespread in eukaryotes. The first described tyrosine recombinase mobile element, DIRS1, is a retroelement from the slime mold Dictyostelium discoideum . The YR elements are bordered by terminal repeats related to their replication via free
Russell T M, Poulter, Margi I, Butler
openaire   +2 more sources

FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae. [PDF]

open access: yesPLoS Genetics, 2008
Unlike most bacteria, Vibrio cholerae harbors two distinct, nonhomologous circular chromosomes (chromosome I and II). Many features of chromosome II are plasmid-like, which raised questions concerning its chromosomal nature.
Marie-Eve Val   +5 more
doaj   +1 more source

Resolution of Mismatched Overlap Holliday Junction Intermediates by the Tyrosine Recombinase IntDOT [PDF]

open access: yesJournal of Bacteriology, 2017
ABSTRACT CTnDOT is an integrated conjugative element found in Bacteroides species. CTnDOT contains and transfers antibiotic resistance genes. The element integrates into and excises from the host chromosome via a Holliday junction (HJ) intermediate as part of a site-specific recombination mechanism.
Kenneth, Ringwald   +2 more
openaire   +2 more sources

Initial characterization of the Pf-Int recombinase from the malaria parasite Plasmodium falciparum.

open access: yesPLoS ONE, 2012
BackgroundGenetic variation is an essential means of evolution and adaptation in many organisms in response to environmental change. Certain DNA alterations can be carried out by site-specific recombinases (SSRs) that fall into two families: the serine ...
Mehdi Ghorbal   +9 more
doaj   +1 more source

The complete plastid genomes of the two 'dinotoms' Durinskia baltica and Kryptoperidinium foliaceum. [PDF]

open access: yesPLoS ONE, 2010
In one small group of dinoflagellates, photosynthesis is carried out by a tertiary endosymbiont derived from a diatom, giving rise to a complex cell that we collectively refer to as a 'dinotom'.
Behzad Imanian   +2 more
doaj   +1 more source

Flipping chromosomes in deep-sea archaea. [PDF]

open access: yesPLoS Genetics, 2017
One of the major mechanisms driving the evolution of all organisms is genomic rearrangement. In hyperthermophilic Archaea of the order Thermococcales, large chromosomal inversions occur so frequently that even closely related genomes are difficult to ...
Matteo Cossu   +7 more
doaj   +1 more source

Atypical integrative element with strand-biased circularization activity assists interspecies antimicrobial resistance gene transfer from Vibrio alfacsensis.

open access: yesPLoS ONE, 2022
The exchange of antimicrobial resistance (AMR) genes between aquaculture and terrestrial microbial populations has emerged as a serious public health concern. However, the nature of the mobile genetic elements in marine bacteria is poorly documented.
Lisa Nonaka   +2 more
doaj   +1 more source

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