Results 21 to 30 of about 179,081 (177)

The XerC recombinase ofProteus mirabilis: characterization and interaction with other tyrosine recombinases [PDF]

FEMS Microbiology Letters, 2003
XerC and XerD are two site-specific recombinases, which act on different sites to maintain replicons in a monomeric state. This system, which was first discovered and studied in Escherichia coli, is present in several species including Proteus mirabilis, where the XerD recombinase was previously characterized by our laboratory. In this paper, we report
Manuela, Villion, George, Szatmari
openaire   +2 more sources

The dif/Xer recombination systems in proteobacteria. [PDF]

PLoS ONE, 2009
In E. coli, 10 to 15% of growing bacteria produce dimeric chromosomes during DNA replication. These dimers are resolved by XerC and XerD, two tyrosine recombinases that target the 28-nucleotide motif (dif) associated with the chromosome's replication ...
Christophe Carnoy, Claude-Alain Roten
doaj   +1 more source

Study of VIPER and TATE in kinetoplastids and the evolution of tyrosine recombinase retrotransposons [PDF]

Mobile DNA, 2019
Kinetoplastids are a flagellated group of protists, including some parasites, such as Trypanosoma and Leishmania species, that can cause diseases in humans and other animals. The genomes of these species enclose a fraction of retrotransposons including VIPER and TATE, two poorly studied transposable elements that encode a tyrosine recombinase (YR) and ...
Yasmin Carla Ribeiro, Lizandra Jaqueline Robe, Danila Syriani Veluza, Cyndia Mara Bezerra dos Santos, Ana Luisa Kalb Lopes, Marco Aurélio Krieger, Adriana Ludwig   +6 more
openaire   +3 more sources

Challenging a Paradigm: the Role of DNA Homology in Tyrosine Recombinase Reactions [PDF]

Microbiology and Molecular Biology Reviews, 2009
SUMMARYA classical feature of the tyrosine recombinase family of proteins catalyzing site-specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp recombinases, is the ability to recombine substrates sharing very limited DNA sequence identity.
Lara, Rajeev, Karolina, Malanowska, Jeffrey F, Gardner   +2 more
openaire   +2 more sources

The carboxy-terminal αN helix of the archaeal XerA tyrosine recombinase is a molecular switch to control site-specific recombination.

PLoS ONE, 2013
Tyrosine recombinases are conserved in the three kingdoms of life. Here we present the first crystal structure of a full-length archaeal tyrosine recombinase, XerA from Pyrococcus abyssi, at 3.0 Å resolution.
Marie-Claude Serre, Toufic El Arnaout, Mark A Brooks, Dominique Durand, Johnny Lisboa, Noureddine Lazar, Bertrand Raynal, Herman van Tilbeurgh, Sophie Quevillon-Cheruel   +8 more
doaj   +1 more source

Zinc finger recombinases with adaptable DNA sequence specificity [PDF]

, 2011
Site-specific recombinases have become essential tools in genetics and molecular biology for the precise excision or integration of DNA sequences. However, their utility is currently limited to circumstances where the sites recognized by the recombinase ...
Proudfoot, C., W. Marshall Stark, W Marshall Stark, Proudfoot Chris, Kolb, A.F., Proudfoot, Chris, Arlene L McPherson, Arlene L. McPherson, Kolb Andreas F., McPherson, A.L., McPherson Arlene L., Andreas F Kolb, McPherson, Arlene L, Proudfoot, Christopher, Andreas F. Kolb, Stark W. Marshall, Stark, W.M., Kolb, Andreas F, Stark, W Marshall, Chris Proudfoot   +19 more
core   +1 more source

Self‐control in DNA site‐specific recombination mediated by the tyrosine recombinase TnpI [PDF]

Molecular Microbiology, 2006
SummaryTn4430is a distinctive transposon of the Tn3family that encodes a tyrosine recombinase (TnpI) to resolve replicative transposition intermediates. The internal resolution site of Tn4430(IRS, 116 bp) contains two inverted repeats (IR1 and IR2) at the crossover core site, and two additional TnpI binding motifs (DR1 and DR2) adjacent to the core ...
Virginie, Vanhooff, Christine, Galloy, Hervé, Agaisse, Didier, Lereclus, Bernard, Révet, Bernard, Hallet   +5 more
openaire   +4 more sources

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Scientific Reports, 2020
The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be
Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro   +3 more
doaj   +1 more source

Determinants of selectivity in Xer site-specific recombination [PDF]

, 1996
A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination
Blakely, G, Sherratt, D
core   +1 more source

Identification and Characterization of a Phase-Variable Element That Regulates the Autotransporter UpaE in Uropathogenic Escherichia coli

mBio, 2018
Uropathogenic Escherichia coli (UPEC) is the most common etiologic agent of uncomplicated urinary tract infection (UTI). An important mechanism of gene regulation in UPEC is phase variation that involves inversion of a promoter-containing DNA element via
E. J. Battaglioli, K. G. K. Goh, T. S. Atruktsang, K. Schwartz, M. A. Schembri, R. A. Welch   +5 more
doaj   +1 more source