The Role of the U5 snRNP in Genetic Disorders and Cancer [PDF]
Frontiers in Genetics, 2021 Pre-mRNA splicing is performed by the spliceosome, a dynamic macromolecular complex consisting of five small uridine-rich ribonucleoprotein complexes (the U1, U2, U4, U5, and U6 snRNPs) and numerous auxiliary splicing factors.
Katherine A. Wood +5 more
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TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation [PDF]
Nature Communications, 2021 The correct assembly and recycling of the multicomponent spliceosome remains largely elusive. Here, the authors show that a previously uncharacterized protein TSSC4 associates with de novo formed spliceosomal U5 snRNP as well as with a post-splicing U5 ...
Klára Klimešová +6 more
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Addressing the tissue specificity of U5 snRNP spliceosomopathies [PDF]
Frontiers in Cell and Developmental BiologyPrecursor mRNA (pre-mRNA) must undergo splicing to remove intron sequences and join exons. This splicing process is catalysed by an RNA/protein complex called the spliceosome.
Rahmat Azhari Kemal +2 more
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Cell cycle abnormalities associated with differential perturbations of the human U5 snRNP associated U5-200kD RNA helicase. [PDF]
PLoS ONE, 2013 Splicing of pre-messenger RNAs into functional messages requires a concerted assembly of proteins and small RNAs that identify the splice junctions and facilitate cleavage of exon-intron boundaries and ligation of exons.
Ali Ehsani +2 more
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The 35S U5 snRNP Is Generated from the Activated Spliceosome during In vitro Splicing. [PDF]
PLoS ONE, 2015 Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive
Olga V Makarova, Evgeny M Makarov
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U5 snRNP Core Proteins Are Key Components of the Defense Response against Viral Infection through Their Roles in Programmed Cell Death and Interferon Induction [PDF]
Viruses, 2022 The spliceosome is a massive ribonucleoprotein structure composed of five small nuclear ribonucleoprotein (snRNP) complexes that catalyze the removal of introns from pre-mature RNA during constitutive and alternative splicing. EFTUD2, PRPF8, and SNRNP200
Simon Boudreault +2 more
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GPATCH11 ortholog Sap34 regulates pre-mRNA splicing by interacting with early spliceosomal complexes in Schizosaccharomyces pombe [PDF]
Scientific ReportsPre-mRNA splicing is an essential step in gene expression regulation. It is mediated by the spliceosome, a large ribonucleoprotein complex that undergoes dynamic structural and compositional rearrangements during each splicing cycle.
Ingrid Cipakova +8 more
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The Dengue Virus NS5 Protein Intrudes in the Cellular Spliceosome and Modulates Splicing. [PDF]
PLoS Pathogens, 2016 Dengue virus NS5 protein plays multiple functions in the cytoplasm of infected cells, enabling viral RNA replication and counteracting host antiviral responses. Here, we demonstrate a novel function of NS5 in the nucleus where it interferes with cellular
Federico A De Maio +12 more
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Nature Communications, 2021 In yeast, TFIP11 and DHX15 promote the disassembly of spliceosome complex after splicing is completed. Here the authors show that human TFIP11 functions independently of DHX15 and is required for U6 snRNA 2’-O-methylation and U4/U6.U5 tri-snRNP assembly.
Amandine Duchemin +14 more
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Identification of a PRPF4 loss-of-function variant that abrogates U4/U6.U5 tri-snRNP integration and is associated with retinitis pigmentosa. [PDF]
PLoS ONE, 2014 Pre-mRNA splicing by the spliceosome is an essential step in the maturation of nearly all human mRNAs. Mutations in six spliceosomal proteins, PRPF3, PRPF4, PRPF6, PRPF8, PRPF31 and SNRNP200, cause retinitis pigmentosa (RP), a disease characterized by ...
Bastian Linder +7 more
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