Results 11 to 20 of about 10,954 (152)
Structure of the human 20S U5 snRNP. [PDF]
Nat Struct Mol BiolAbstractThe 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA–protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP.
Schneider S +8 more
europepmc +5 more sources
Reovirus μ2 Protein Impairs Translation to Reduce U5 snRNP Protein Levels. [PDF]
Int J Mol Sci, 2022 Mammalian orthoreovirus (MRV) is a double-stranded RNA virus from the Reoviridae family that infects a large range of mammals, including humans. Recently, studies have shown that MRV alters cellular alternative splicing (AS) during viral infection. The structural protein μ2 appears to be the main determinant of these AS modifications by decreasing the ...
Boudreault S +5 more
europepmc +4 more sources
Structural basis for dual roles of Aar2p in U5 snRNP assembly. [PDF]
Genes Dev, 2013 Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase. Instead, pre-U5 snRNP includes the Aar2 protein not found in mature U5 snRNP or spliceosomes.
Weber G +8 more
europepmc +8 more sources
Investigation into the Saccharomyces cerevisiae U5 snRNP, a core spliceosome component [PDF]
, 2010 The U5 snRNP is a major component of the yeast spliceosome, being part of the U4/U6.U5 tri-snRNP, the precatalytic spliceosome and the catalytically activated spliceosome. The U5 snRNP includes, at its heart, the U5 snRNA which contains the invariant Loop 1 that functions in tethering and aligning exons during splicing.
Nancollis, Verity
core +14 more sources
Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones. [PDF]
J Cell Biol, 2017 Splicing is catalyzed by the spliceosome, a complex of five major small nuclear ribonucleoprotein particles (snRNPs). The pre-mRNA splicing factor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a central module of the spliceosome.
Malinová A +9 more
europepmc +7 more sources
Mechanism for Aar2p function as a U5 snRNP assembly factor. [PDF]
Genes Dev, 2011 Little is known about how particle-specific proteins are assembled on spliceosomal small nuclear ribonucleoproteins (snRNPs). Brr2p is a U5 snRNP-specific RNA helicase required for spliceosome catalytic activation and disassembly. In yeast, the Aar2 protein is part of a cytoplasmic precursor U5 snRNP that lacks Brr2p and is replaced by Brr2p in the ...
Weber G +7 more
europepmc +7 more sources
Structural Basis for the Bifunctionality of the U5 snRNP 52K Protein (CD2BP2) [PDF]
Journal of Molecular Biology, 2007 The bifunctional protein U5-52K is associated with the spliceosomal 20 S U5 snRNP, and it also plays a role in immune response as CD2 receptor binding protein 2 (CD2BP2). U5-52K binds to the CD2 receptor via its GYF-domain specifically recognizing a proline-rich motif on the cytoplasmic surface of the receptor.
Nielsen, Tine Kragh +3 more
openaire +6 more sources
Molecular architecture of the human U4/U6.U5 tri-snRNP [PDF]
Science, 2016 A human spliceosomal subcomplex The spliceosome is an RNA and protein molecular machine that cuts out introns from messenger RNAs. Agafonov et al. used cryo-electron microscopy to determine the structure of the largest intermediate subcomplex on the assembly pathway for the ...
Agafonov, Dmitry E. +7 more
openaire +5 more sources
Ecd promotes U5 snRNP maturation and Prp8 stability. [PDF]
Nucleic Acids Res, 2021 Abstract Pre-mRNA splicing catalyzed by the spliceosome represents a critical step in the regulation of gene expression contributing to transcriptome and proteome diversity. The spliceosome consists of five small nuclear ribonucleoprotein particles (snRNPs), the biogenesis of which remains only partially understood.
Erkelenz S +7 more
europepmc +4 more sources
The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly. [PDF]
J Cell Biochem, 2013 The U5 small nuclear ribonucleoprotein particle (snRNP) forms the heart of the spliceosome which is required for intron removal from pre-mRNA. The proteins Prp8, Snu114 and Brr2 all assemble with the U5 small nuclear RNA (snRNA) to produce the U5 snRNP ...
Nancollis V +3 more
europepmc +2 more sources