Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy [PDF]
Molecular Cell, 2006 In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome.
Sander, Bjoern +6 more
openaire +4 more sources
Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
doaj +1 more source
MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis
Life Science Alliance, 2023 MDF acts like its human homolog SART1 as a splicing factor that is important for maintenance of genome stability and correct splicing and expression of genes involved in cell division and stress response.
Cloe de Luxán-Hernández +6 more
doaj +1 more source
Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly. [PDF]
Nucleic Acids Research, 1991 The pre-mRNA splicing pathway is highly conserved from yeast (S. cerevisiae) to mammals. Of the four snRNPs involved in splicing three (U1, U2 and U4/U6) have been shown to be essential for in vitro splicing. To examine the remaining snRNP, we utilized our previously described genetic procedures (Seraphin and Rosbash, 1989) to prepare yeast extracts ...
Bertrand Séraphin +2 more
semanticscholar +3 more sources
TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes. [PDF]
PLoS Genetics, 2017 Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility.
Müge Akpınar +6 more
doaj +1 more source
Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. [PDF]
PLoS ONE, 2012 Splicing of primary transcripts is an essential process for the control of gene expression. Specific conserved sequences in premature transcripts are important to recruit the spliceosome machinery.
Patricia P Coltri, Carla C Oliveira
doaj +1 more source
The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis
Frontiers in ImmunologyThe question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon +19 more
doaj +1 more source
eLife, 2016 The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing
André Mourão +6 more
doaj +1 more source
Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing. [PDF]
PLoS Genetics, 2014 The steroid hormone ecdysone coordinates insect growth and development, directing the major postembryonic transition of forms, metamorphosis. The steroid-deficient ecdysoneless1 (ecd1) strain of Drosophila melanogaster has long served to assess the ...
Ann-Katrin Claudius +4 more
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Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals [PDF]
The EMBO Journal, 1997 The first AG dinucleotide downstream from the branchpoint sequence (BPS) is chosen as the 3' splice site during catalytic step II of the splicing reaction. The mechanism and factors involved in selection of this AG are not known. Early in mammalian spliceosome assembly, U2AF65 binds to the pyrimidine tract between the BPS and AG.
M. Chiara +3 more
semanticscholar +3 more sources