Results 41 to 50 of about 10,954 (152)

Organization of Core Spliceosomal Components U5 snRNA Loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as Revealed by Electron Cryomicroscopy [PDF]

Molecular Cell, 2006
In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome.
Sander, Bjoern, Golas, Monika M., Makarov, Evgeny M., Brahms, Hero, Kastner, Berthold, Lührmann, Reinhard, Stark, Holger   +6 more
openaire   +4 more sources

Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy

Cell Reports, 2013
Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova, Aaron A. Hoskins, Larry J. Friedman, Victor Serebrov, Ivan R. Corrêa, Jr., Ming-Qun Xu, Jeff Gelles, Melissa J. Moore   +7 more
doaj   +1 more source

MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis

Life Science Alliance, 2023
MDF acts like its human homolog SART1 as a splicing factor that is important for maintenance of genome stability and correct splicing and expression of genes involved in cell division and stress response.
Cloe de Luxán-Hernández, Julia Lohmann, Eduardo Tranque, Jana Chumova, Pavla Binarova, Julio Salinas, Magdalena Weingartner   +6 more
doaj   +1 more source

Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain [PDF]

RNA, 2006
Snu114 is a U5 snRNP protein essential for pre-mRNA splicing. Based on its homology with the ribosomal translocase EF-G, it is thought that GTP hydrolysis by Snu114 induces conformational rearrangements in the spliceosome. We recently identified allele-specific genetic interactions between SNU114 and genes encoding three other U5 snRNP components, Prp8
Tamara J, Brenner, Christine, Guthrie
openaire   +2 more sources

TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes. [PDF]

PLoS Genetics, 2017
Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility.
Müge Akpınar, Mathias Lesche, Grigorios Fanourgakis, Jun Fu, Konstantinos Anastassiadis, Andreas Dahl, Rolf Jessberger   +6 more
doaj   +1 more source

Isoforms of U1-70k control subunit dynamics in the human spliceosomal U1 snRNP [PDF]

, 2009
Most human protein-encoding genes contain multiple exons that are spliced together, frequently in alternative arrangements, by the spliceosome. It is established that U1 snRNP is an essential component of the spliceosome, in human consisting of RNA and ...
Nina Morgner, Hernández Helena, Makarov Evgeny M., H. Hernández (7607219), Yutaka Muto, D. P. Krummel (7607234), Helena Hernández, Carol V Robinson, O. V. Makarova (7607222), Robinson, CV, Krummel, DP, Daniel Pomeranz Krummel, Hernandez, H, Evgeny M Makarov, Makarova, OV, Y. Muto (7607231), Olga V Makarova, Robinson Carol V., E. M. Makarov (7607225), N. Morgner (7607228), Makarov, EM, Robinson, Carol, Makarova Olga V., Muto Yutaka, Krummel Daniel Pomeranz, C. V. Robinson (7607237), Morgner, N, Morgner Nina, Muto, Y   +28 more
core   +1 more source

Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. [PDF]

PLoS ONE, 2012
Splicing of primary transcripts is an essential process for the control of gene expression. Specific conserved sequences in premature transcripts are important to recruit the spliceosome machinery.
Patricia P Coltri, Carla C Oliveira
doaj   +1 more source

The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis

Frontiers in Immunology
The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon, Almudena Hurtado-Pico, Carlos Plaza-Sirvent, Marc Schuster, Marco Preußner, Benno Kuropka, Fan Liu, Andor Zenon Amandus Kirsten, Xiao Jakob Schmitt, Benjamin König, Miguel Álvaro-Benito, Miguel Álvaro-Benito, Esam T. Abualrous, Esam T. Abualrous, Esam T. Abualrous, Gesa I. Albert, Stefanie Kliche, Florian Heyd, Ingo Schmitz, Christian Freund   +19 more
doaj   +1 more source

Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation

eLife, 2016
The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing
André Mourão, Sophie Bonnal, Komal Soni, Lisa Warner, Rémy Bordonné, Juan Valcárcel, Michael Sattler   +6 more
doaj   +1 more source

Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing. [PDF]

PLoS Genetics, 2014
The steroid hormone ecdysone coordinates insect growth and development, directing the major postembryonic transition of forms, metamorphosis. The steroid-deficient ecdysoneless1 (ecd1) strain of Drosophila melanogaster has long served to assess the ...
Ann-Katrin Claudius, Patrizia Romani, Tobias Lamkemeyer, Marek Jindra, Mirka Uhlirova   +4 more
doaj   +1 more source