Results 21 to 30 of about 10,954 (152)

Structural basis of human U5 snRNP late biogenesis and recycling. [PDF]

Nat Struct Mol Biol
Pre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP is the ~1 MDa 'heart' of the spliceosome and is recycled through an unknown mechanism involving major architectural rearrangements and the dedicated ...
Riabov Bassat D, Visanpattanasin S, Vorländer MK, Fin L, Phillips AW, Plaschka C.   +5 more
europepmc   +3 more sources

The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation [PDF]

RNA, 2005
The U5 snRNP plays an essential role in both U2- and U12-dependent splicing. Here, we have characterized a 52-kDa protein associated with the human U5 snRNP, designated U5-52K. Protein sequencing revealed that U5-52K is identical to the CD2BP2, which interacts with the cytoplasmic portion of the human T-cell surface protein CD2.
Laggerbauer, B., Liu, S., Makarov, E., Vornlocher, H., Makarova, O., Ingelfinger, D., Achsel, T., Luehrmann, R.   +7 more
openaire   +3 more sources

Study into a role for Aar2p in U5 snRNP biogenesis [PDF]

, 2011
Aar2p is an essential yeast protein involved in pre-mRNA splicing and component of a U5 snRNP precursor form. It has been suggested that the mature U5 snRNP and U4/U6.U5 tri-snRNP assemble from the Aar2p-U5 core particle that is formed in the ...
Cristao, Vanessa Solange Fernandes de Oliveira
core   +3 more sources

The architecture of the spliceosomal U4/U6.U5 tri-snRNP [PDF]

Nature, 2015
U4/U6.U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal complex comprising U5 small nuclear RNA (snRNA), extensively base-paired U4/U6 snRNAs and more than 30 proteins, including the key components Prp8, Brr2 and Snu114. The tri-snRNP combines with a precursor messenger RNA substrate bound to U1 and U2 small nuclear ribonucleoprotein ...
Nguyen, Thi Hoang Duong, Galej, Wojciech P., Bai, Xiao-chen, Savva, Christos G., Newman, Andrew J., Scheres, Sjors H. W., Nagai, Kiyoshi   +6 more
openaire   +2 more sources

The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA [PDF]

Chromosome Research, 2012
In this study, we take advantage of the high spatial resolution offered by the nucleus and lampbrush chromosomes of the amphibian oocyte to investigate the mechanisms that regulate the intranuclear trafficking of the U5 snRNP and its recruitment to nascent transcripts.
Rebecca, Kim, Joshua, Paschedag, Natalya, Novikova, Michel, Bellini   +3 more
openaire   +2 more sources

Role of Cajal bodies and nucleolus in the maturation of the U1 snRNP in Arabidopsis. [PDF]

PLoS ONE, 2008
BACKGROUND: The biogenesis of spliceosomal snRNPs takes place in both the cytoplasm where Sm core proteins are added and snRNAs are modified at the 5' and 3' termini and in the nucleus where snRNP-specific proteins associate.
Zdravko J Lorković, Andrea Barta
doaj   +1 more source

The spliceosome component Usp39 controls B cell development by regulating immunoglobulin gene rearrangement

Cell Reports, 2022
Summary: The spliceosome is a large ribonucleoprotein complex responsible for pre-mRNA splicing and genome stability maintenance. Disruption of the spliceosome activity may lead to developmental disorders and tumorigenesis.
Gui-Xin Ruan, Yuxing Li, Wenjing Chen, Hengjun Huang, Rui Zhang, Changxu Chen, Kong-Peng Lam, Shengli Xu, Xijun Ou   +8 more
doaj   +1 more source

Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8 [PDF]

, 2009
The U5 snRNA loop 1 aligns the 5' and 3' exons for ligation during the second step of pre-mRNA splicing. U5 is intimately associated with Prp8, which mediates pre-mRNA repositioning within the catalytic core of the spliceosome and interacts directly with
David Barrass, J., Kershaw, Christopher J., O'Keefe, Raymond T., Barrass, J. David, Beggs, Jean D., O'Keefe, Raymond T.; id_orcid   +5 more
core   +1 more source

New insights into trypanosomatid U5 small nuclear ribonucleoproteins

Memorias do Instituto Oswaldo Cruz, 2011
Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems
Marco Túlio A da Silva, Daniela L Ambrósio, Caroline C Trevelin, Tatiana F Watanabe, Helen J Laure, Lewis J Greene, José C Rosa, Sandro R Valentini, Regina MB Cicarelli   +8 more
doaj   +1 more source

Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs [PDF]

, 2011
Copyright @ 2011 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use ...
E. M. Makarov (7607225), Evgeny M. Makarov, Andrew Bottrill, O. V. Makarova (7607222), Makarov, EM, Bottrill, A, N. Owen (7618997), Owen, N, Olga V. Makarova, Makarova, OV, A. Bottrill (7622258), Nicholas Owen   +11 more
core   +1 more source