Results 61 to 70 of about 10,954 (152)

Evidence that U5 snRNP recognizes the 3′ splice site for catalytic step II in mammals [PDF]

The EMBO Journal, 1997
The first AG dinucleotide downstream from the branchpoint sequence (BPS) is chosen as the 3' splice site during catalytic step II of the splicing reaction. The mechanism and factors involved in selection of this AG are not known. Early in mammalian spliceosome assembly, U2AF65 binds to the pyrimidine tract between the BPS and AG.
M D, Chiara, L, Palandjian, R, Feld Kramer, R, Reed   +3 more
openaire   +2 more sources

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly [PDF]

Nucleic Acids Research, 1991
The pre-mRNA splicing pathway is highly conserved from yeast (S. cerevisiae) to mammals. Of the four snRNPs involved in splicing three (U1, U2 and U4/U6) have been shown to be essential for in vitro splicing. To examine the remaining snRNP, we utilized our previously described genetic procedures (Seraphin and Rosbash, 1989) to prepare yeast extracts ...
B, Séraphin, N, Abovich, M, Rosbash
openaire   +2 more sources

In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies

Molecular Biology of the Cell, 2011
The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of
Novotný, I. (Ivan), Blažíková, M. (Michaela), Staněk, D. (David), Heřman, P., Malínský, J. (Jan)   +4 more
openaire   +3 more sources

The network of protein–protein interactions within the human U4/U6.U5 tri-snRNP [PDF]

RNA, 2006
The human 25S U4/U6.U5 tri-snRNP is a major building block of the U2-type spliceosome and contains, in addition to the U4, U6, and U5 snRNAs, at least 30 distinct proteins. To learn more about the molecular architecture of the tri-snRNP, we have investigated interactions between tri-snRNP proteins using the yeast two-hybrid assay and in vitro binding ...
Liu, S., Rauhut, R., Vornlocher, H., Luehrmann, R.   +3 more
openaire   +3 more sources

Genetic screening for Arabidopsis mutants defective in STA1 regulation under thermal stress implicates the existence of regulators of its specific expression, and the genetic interactions in the stress signaling pathways

Frontiers in Plant Science, 2016
To cope with environmental stresses, plants have developed various stress tolerance mechanisms that involve the induction of many stress responsive genes through stress-specific and common signaling pathways. Stress-specific/common transcription factors,
Si-in eYu, Jin-Hee eHan, Chanvotey eChhoeun, Byeong-ha eLee   +3 more
doaj   +1 more source

Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies [PDF]

The Journal of cell biology, 1992
We have recently shown that discrete foci are present in the nuclei of mammalian cells in which each of the U1, U2, U4/U6, and U5 snRNPs involved in pre-mRNA splicing, and the non-snRNP-splicing factor U2AF, are concentrated (Carmo-Fonseca, M., D. Tollervey, R. Pepperkok, S. Barabino, A. Merdes, C. Brunner, P. D. Zamore, M. R. Green, E. Hurt, and A. I.
Carmo-Fonseca, M., Pepperkok, R., Carvalho, M. T., Lamond, A. I.   +3 more
openaire   +3 more sources

A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II [PDF]

, 2014
Cajal bodies are nuclear structures that are involved in biogenesis of snRNPs and snoRNPs, maintenance of telomeres and processing of histone mRNA. Recently, the SUMO isopeptidase USPL1 was identified as a component of Cajal bodies that is essential for ...
Winter, Ulrike, Hutten, Saskia, Melchior, Frauke, Chachami, Georgia, Lamond, Angus I.; id_orcid   +4 more
core   +1 more source

Sad1 Counteracts Brr2-Mediated Dissociation of U4/U6.U5 in Tri-snRNP Homeostasis [PDF]

Molecular and Cellular Biology, 2014
The yeast Sad1 protein was previously identified in a screen for factors involved in the assembly of the U4/U6 di-snRNP particle. Sad1 is required for pre-mRNA splicing both in vivo and in vitro, and its human orthologue has been shown to associate with U4/U6.U5 tri-snRNP.
Yu-Hsin, Huang, Che-Sheng, Chung, Der-I, Kao, Tzu-Chung, Kao, Soo-Chen, Cheng   +4 more
openaire   +2 more sources

Nuclear Condensates Of Wac Regulate Mitophagy Through Alternative Splicing

International Dental Journal
Aim or purpose: The present study aimed to investigate the nuclear condensates formation ability of Desanto-Shinawi syndrome (DESSH) related protein-WW domain-containing adaptor with coiled-coil (WAC) and related biological function.
Jiahe Wang, Chenchen Zhou
doaj   +1 more source

Alternative splicing regulation by tumor suppressing subtransferable candidate 4: a pathway to tumor suppression

Frontiers in Immunology
IntroductionRNA splicing is a crucial posttranscriptional process that governs gene expression, and defects in alternative splicing contribute to various diseases, including cancer.
Haiping Zhao, Nana Wu, Gaigai Wei, Huiling Zhang, Tingrong Ren, Jingjing Yi, Yuqi Zhang, Zixi Wang, Yihan Wang, Zhihan Guo, Duanwu Zhang   +10 more
doaj   +1 more source