Results 51 to 60 of about 8,536 (192)

Antisense oligonucleotide binding to U5 snRNP induces a conformational change that exposes the conserved loop of U5 snRNA [PDF]

Nucleic Acids Research, 1997
Conformational rearrangements of the spliceosomal small nuclear RNAs (U snRNAs) are essential for proper assembly of the active site prior to the first catalytic step of splicing. We have previously shown that conformational changes caused by binding of an antisense 2'-O-methyl RNA oligonucleotide (BU5Ae) to U5 snRNA nt 68-88 disrupted the U4/U5/U6 ...
G, Ast, A M, Weiner
openaire   +2 more sources

Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation

eLife, 2016
The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing
André Mourão, Sophie Bonnal, Komal Soni, Lisa Warner, Rémy Bordonné, Juan Valcárcel, Michael Sattler   +6 more
doaj   +1 more source

A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts [PDF]

, 2005
Gene expression is a multistep process starting in the cell nucleus with the synthesis of the primary transcripts that undergo several modifications (including capping, splicing, and polyadenylation) leading to the export of the mature mRNAs into the ...
Auboeuf, Didier, Dowhan, Dennis H., Dutertre, Martin, Martin, Natalia, Berget, Susan M., O'Malley, Bert W.   +5 more
core   +1 more source

The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis

Frontiers in Immunology
The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon, Almudena Hurtado-Pico, Carlos Plaza-Sirvent, Marc Schuster, Marco Preußner, Benno Kuropka, Fan Liu, Andor Zenon Amandus Kirsten, Xiao Jakob Schmitt, Benjamin König, Miguel Álvaro-Benito, Miguel Álvaro-Benito, Esam T. Abualrous, Esam T. Abualrous, Esam T. Abualrous, Gesa I. Albert, Stefanie Kliche, Florian Heyd, Ingo Schmitz, Christian Freund   +19 more
doaj   +1 more source

Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes

Cell Reports, 2017
Summary: Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5′ to 3′ mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer.
Caroline Vindry, Aline Marnef, Helen Broomhead, Laure Twyffels, Sevim Ozgur, Georg Stoecklin, Miriam Llorian, Christopher W. Smith, Juan Mata, Dominique Weil, Nancy Standart   +10 more
doaj   +1 more source

evolution, structure and function of metazoan splicing factor PRPF39 [PDF]

, 2019
In the yeast U1 snRNP the Prp39/Prp42 heterodimer is essential for early steps of spliceosome assembly. In metazoans no Prp42 ortholog exists, raising the question how the heterodimer is functionally substituted.
Bortoli, Francesca De, Heyd, Florian, Kotte, Ana, Loll, Bernhard, Neumann, Alexander, Schüler, Thomas, Timmermann, Bernd, Wahl, Markus C.   +7 more
core   +1 more source

Human U4/U6.U5 and U4atac/U6atac.U5 Tri-snRNPs Exhibit Similar Protein Compositions [PDF]

Molecular and Cellular Biology, 2002
In the U12-dependent spliceosome, the U4atac/U6atac snRNP represents the functional analogue of the major U4/U6 snRNP. Little information is available presently regarding the protein composition of the former snRNP and its association with other snRNPs.
Schneider C, Will CL, Makarova OV, Makarov EM, Lührmann R   +4 more
openaire   +4 more sources

Spliceosome factors target timeless (tim) mRNA to control clock protein accumulation and circadian behavior in Drosophila

eLife, 2018
Transcription-translation feedback loops that comprise eukaryotic circadian clocks rely upon temporal delays that separate the phase of active transcription of clock genes, such as Drosophila period (per) and timeless (tim), from negative feedback by the
Iryna Shakhmantsir, Soumyashant Nayak, Gregory R Grant, Amita Sehgal   +3 more
doaj   +1 more source

Dual Targeting of Mutant p53 and SNRPD2 via Engineered Exosomes Modulates Alternative Splicing to Suppress Ovarian Cancer

Advanced Science, Volume 13, Issue 17, 23 March 2026.
Mutant p53 drives oncogenic splicing to promote the progression of ovarian cancer by partnering with the spliceosome factor SNRPD2. Therefore, it is engineered iRGD‐exosomes to co‐deliver siRNAs against both targets. This approach restored tumor‐suppressive mRNA isoforms, effectively enhanced sensitivity to cisplatin, and ultimately blocked tumor ...
Wei Zhao, Qian Hao, Yu Gan, Jing Tong, Xiaodan Chen, Shuran Tan, Ruiwen Ruan, Yingdan Huang, Mingming Cao, Jun Deng, Tao Han, Getao Shi, Bo Gao, Yu Zhang, Xiang Zhou   +14 more
wiley   +1 more source

Anti-U5 snRNP antibody as a possible serological marker for scleroderma-polymyositis overlap [PDF]

Rheumatology, 2002
To determine the prevalence of the anti-U5 small nuclear ribonucleoprotein (snRNP) antibody in patients with systemic sclerosis.Sera from 281 patients with systemic sclerosis, including 10 patients with overlapping polymyositis, were assayed using RNA immunoprecipitation and protein immunoprecipitation.Only one serum sample showed precipitation of U5 ...
M, Kubo, H, Ihn, M, Kuwana, Y, Asano, T, Tamaki, K, Yamane, K, Tamaki   +6 more
openaire   +2 more sources