Results 81 to 90 of about 8,536 (192)

Multiple splicing factors are released from endogenous complexes during in vitro pre-mRNA splicing [PDF]

, 1989
Pre-mRNA splicing occurs in a macromolecular complex called the spliceosome. Efforts to isolate spliceosomes from in vitro splicing reactions have been hampered by the presence of endogenous complexes that copurify with de novo spliceosomes formed on ...
Conway, G. C., Krainer, A. R., Spector, D. L., Roberts, R. J.   +3 more
core  

In vivo kinetics of U4/U6·U5 tri-snRNP formation in Cajal bodies

Molecular Biology of the Cell, 2011
The U4/U6·U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) is an essential pre-mRNA splicing factor, which is assembled in a stepwise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs), but little is known about the dynamics of this process. Here we created a mathematical model of
Novotný, I. (Ivan), Blažíková, M. (Michaela), Staněk, D. (David), Heřman, P., Malínský, J. (Jan)   +4 more
openaire   +3 more sources

Combinatorial Analyses of Pre‐mRNA Splicing‐Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions

Genes to Cells, Volume 30, Issue 6, November 2025.
Different telomere functions show distinct dependence on Rap1 protein levels revealed by analyses of pre‐mRNA splicing mutants. ABSTRACT Telomeres perform multiple functions to maintain genome stability, including telomere length regulation, chromosome end protection, and meiotic chromosome dynamics.
Miho Takeuchi, Yoko Otsubo, Junko Kanoh
wiley   +1 more source

Genetic screening for Arabidopsis mutants defective in STA1 regulation under thermal stress implicates the existence of regulators of its specific expression, and the genetic interactions in the stress signaling pathways

Frontiers in Plant Science, 2016
To cope with environmental stresses, plants have developed various stress tolerance mechanisms that involve the induction of many stress responsive genes through stress-specific and common signaling pathways. Stress-specific/common transcription factors,
Si-in eYu, Jin-Hee eHan, Chanvotey eChhoeun, Byeong-ha eLee   +3 more
doaj   +1 more source

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells. [PDF]

Genes & Development, 1991
We describe the isolation and biochemical characterization of [U4/U6.U5] tri-snRNP complexes from HeLa cells under nondenaturing conditions using a monoclonal antibody reacting with the U5-specific 100-kD protein. We show that the [U4/U6.U5] complex contains five previously unobserved proteins with molecular masses of 90, 60, 27, 20, and 15.5 kD, in ...
S E, Behrens, R, Lührmann
openaire   +2 more sources

Spliceosome protein alterations differentiate hubs of the default mode connectome during the progression of Alzheimer's disease

Brain Pathology, Volume 35, Issue 5, September 2025.
Default mode network (DMN) consists, in part, of the frontal (FC), precuneus (PreC), and posterior cingulate cortex (PCC), which plays a critical role in episodic memory and retrieval of autobiographical memories, displays spatiotemporal differences in the onset of amyloid and hypometabolism that reflects tau neurodegeneration and loss of connectivity ...
Sylvia E. Perez, Muhammad Nadeem, Bin He, Jennifer C. Miguel, David G. Moreno, Marta Moreno‐Rodriguez, Michael Malek‐Ahmadi, Chadwick M. Hales, Elliott J. Mufson   +8 more
wiley   +1 more source

Mutational screening of splicing factor genes in cases with autosomal dominant retinitis pigmentosa [PDF]

, 2014
Purpose Mutations in genes encoding proteins from the tri-snRNP complex of the spliceosome account for more than 12% of cases of autosomal dominant retinitis pigmentosa (adRP).
Ascari, Giulia, Avila-Fernandez, Almudena, Ayuso, Carmen, Benaglio, Paola, Berson, Eliot L., Hamel, Christian, Harper, Shyana, Manes, Gaël, Rivolta, Carlo, San Jose, Patricia Fernandez   +9 more
core   +4 more sources

Transcriptional regulation of PRPF31 gene expression by MSR1 repeat elements causes incomplete penetrance in retinitis pigmentosa. [PDF]

, 2016
PRPF31-associated retinitis pigmentosa presents a fascinating enigma: some mutation carriers are blind, while others are asymptomatic. We identify the major molecular cause of this incomplete penetrance through three cardinal features: (1) there is ...
Bhattacharya, S.S., Chakravarti, A., Krishna, A., Rivolta, C., Rose, A.M., Shah, A.Z., Venturini, G.   +6 more
core   +4 more sources

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution [PDF]

Nature, 2016
U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs).
Nguyen, Thi Hoang Duong, Galej, Wojciech P., Bai, Xiao-chen, Oubridge, Chris, Newman, Andrew J., Scheres, Sjors H. W., Nagai, Kiyoshi   +6 more
openaire   +2 more sources

Alternative Splicing Regulation in Metabolic Disorders

Obesity Reviews, Volume 26, Issue 9, September 2025.
ABSTRACT Alternative splicing (AS) is a fundamental mechanism for enhancing transcriptome diversity and regulating gene expression, crucial for various cellular processes and the development of complex traits. This review examines the role of AS in metabolic disorders, including obesity, weight loss, dyslipidemias, and metabolic syndrome.
Dorota Kaminska
wiley   +1 more source