Results 71 to 80 of about 617,710 (283)

Targeting the ubiquitin system by fragment-based drug discovery

Frontiers in Molecular Biosciences, 2022
The ubiquitin system contains a wealth of potential drug targets for many diseases and conditions, including neurodegenerative, immune, metabolic and developmental diseases, as well as multiple cancers.
Cassandra Kennedy, Katherine McPhie, Katrin Rittinger   +2 more
doaj   +1 more source

The lysine deprotonation mechanism in a ubiquitin conjugating enzyme

The Journal of Physical Chemistry B
Ubiquitination is a biochemical reaction in which a small protein, ubiquitin (Ub), is covalently linked to a lysine on a target protein. This type of post-translational modification can signal for protein degradation, DNA repair, or inflammation response.
Alexis J. Wathan, Nicole M. Deschene, Joseph M. Litz, Isaiah Sumner   +3 more
openaire   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

The ubiquitin–proteasome system: A potential target for the MASLD

Acta Pharmaceutica Sinica B
Metabolic dysfunction-associated steatotic liver disease (MASLD), the most prevalent chronic liver condition globally, lacks adequate and effective therapeutic remedies in clinical practice.
Yue Liu, Meijia Qian, Yonghao Li, Xin Dong, Yulian Wu, Tao Yuan, Jian Ma, Bo Yang, Hong Zhu, Qiaojun He   +9 more
doaj   +1 more source

Put a RING on it: Regulation and inhibition of RNF8 and RNF168 RING finger E3 ligases at DNA damage sites.

Frontiers in Genetics, 2013
RING domain containing E3 ubiquitin ligases comprise a large family of enzymes that in combination with an E2 ubiquitin-conjugating enzyme, modify target proteins by attaching ubiquitin moieties.
Cristina eBartocci, Eros eLazzerini Denchi   +1 more
doaj   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Huntingtin Is Ubiquitinated and Interacts with a Specific Ubiquitin-conjugating Enzyme [PDF]

Journal of Biological Chemistry, 1996
Using the yeast two-hybrid system, we have identified a human ubiquitin-conjugating enzyme (hE2-25K) as a protein that interacts with the gene product for Huntington disease (HD) (Huntingtin). This protein has complete amino acid identity with the bovine E2-25K protein and has striking similarity to the UBC-1, -4 and -5 enzymes of Saccharomyces ...
M A, Kalchman, R K, Graham, G, Xia, H B, Koide, J G, Hodgson, K C, Graham, Y P, Goldberg, R D, Gietz, C M, Pickart, M R, Hayden   +9 more
openaire   +2 more sources

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Following Ariadne's thread: a new perspective on RBR ubiquitin ligases

BMC Biology, 2012
Ubiquitin signaling pathways rely on E3 ligases for effecting the final transfer of ubiquitin from E2 ubiquitin conjugating enzymes to a protein target. Here we re-evaluate the hybrid RING/HECT mechanism used by the E3 family RING-between-RINGs (RBRs) to
Wenzel Dawn M, Klevit Rachel E
doaj   +1 more source