Results 61 to 70 of about 299,277 (219)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The Shigella flexneri effector IpaH1.4 facilitates RNF213 degradation and protects cytosolic bacteria against interferon-induced ubiquitylation

eLife
A central signal that marshals host defense against many infections is the lymphocyte-derived cytokine interferon-gamma (IFNγ). The IFNγ receptor is expressed on most human cells, and its activation leads to the expression of antimicrobial proteins that ...
Luz Saavedra-Sanchez, Mary S Dickinson, Shruti S Apte, Yifeng Zhang, Maarten De Jong, Samantha Skavicus, Nicholas S Heaton, Neal M Alto, Jorn Coers   +8 more
doaj   +1 more source

Effects of arachidonic and docosahexaenoic acids on secretion and degradation of bile salt-dependent lipase in AR4-2J cells

Journal of Lipid Research, 2001
In this study we demonstrated that two polyunsaturated fatty acids, arachidonic acid (AA, n–6) and docosahexaenoic acid (DHA, n–3), modulate the secretion of bile salt-dependent lipase (BSDL) by pancreatic AR4-2J cells.
Josette Le Petit-Thévenin, Nadine Bruneau, Alain Nganga, Dominique Lombardo, Alain Vérine   +4 more
doaj   +1 more source

SPG4 and Dementia: Expanding the Clinical Spectrum

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza, Arun Meyyazhagan, Eliseo Picchi, Gustavo Ribas, Ingrid Faber, Ryosuke Miyamoto, Preethi Basavaraju, Paolo Eusebi, Haripriya Kuchi Bhotla, Mario Stasi, Fabrizio Gaudiello, Francesco Patti, Filippo Maria Santorelli, Marcondes Cavalcante França Jr, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Hélio Afonso Ghizoni Teive, Peter Henry St George‐Hyslop, Toshitaka Kawarai, Antonio Orlacchio   +19 more
wiley   +1 more source

Histopathological Evidence of Neurodegenerative Pathology in Epilepsy: A Systematic Review

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Epilepsy affects > 50 million people worldwide and is associated with a disproportionate burden of cognitive impairment. Emerging evidence suggests that neurodegenerative proteinopathies, particularly hyperphosphorylated tau (p‐tau) and amyloid‐β (Aβ), may contribute to cognitive dysfunction in people with epilepsy (PWE), even in the absence ...
Syeda Amrah Hashmi, Aleksander Luniewski, Vanessa Smith, Matthew McCord, Jaideep Kapur, Mark Quigg, Catherine Joshua, Ifrah Zawar   +7 more
wiley   +1 more source

Engineering Nanoplatforms for Alzheimer's Disease Detection via Biomolecular Corona Proteomic and Lipidomic Profiling

Advanced Healthcare Materials, EarlyView.
Engineered nanoparticles capture disease‐specific biomolecular coronas that uncover hidden molecular features of Alzheimer's disease. Combined proteomic and lipidomic analyses reveal a characteristic shift in ribosomal machinery and energy metabolism, generating a multiomic fingerprint that supports accurate disease detection and opens new ...
Antonietta Greco, Roberto Baretta, Andrea Di Fonzo, Andrea Armirotti, Stefano Cinti, Mara Mirasoli, Marco Frasconi, Claudia Corbo   +7 more
wiley   +1 more source

The good, the bad, and the modified: CYLD’s post-translational tale

Frontiers in Cell Death
The deubiquitinating enzyme CYLD hydrolyzes Lys63-and Met1-linked ubiquitin chains, playing a crucial role in regulating various cellular processes such as immune cell development, innate and adaptive immunity, spermatogenesis, ciliogenesis, and cell ...
Yanis M. Macé, Yanis M. Macé, Nicolas Bidère, Nicolas Bidère, Tiphaine Douanne, Tiphaine Douanne   +5 more
doaj   +1 more source

DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions

Cell Reports, 2016
We adapted the yeast 2-hybrid assay to simultaneously uncover multiple transient protein interactions within a single screen by using a strategy termed DEEPN (dynamic enrichment for evaluation of protein networks).
Natasha Pashkova, Tabitha A. Peterson, Venkatramanan Krishnamani, Patrick Breheny, Mark Stamnes, Robert C. Piper   +5 more
doaj   +1 more source

Intravital Multimodal Imaging of Human Cortical Organoid Transplantation in a Mouse Model of Chronic Stroke

Advanced Science, EarlyView.
A multimodal intravital imaging platform enables longitudinal tracking of human cortical organoids transplanted into chronic stroke lesions. By combining surgical microscopy, MRI, bioluminescence imaging, and two‐photon fluorescence microscopy, the platform captures graft placement, viability dynamics, and cellular‐scale morphology in vivo, offering a ...
Jinghui Wang, Guanda Qiao, Honglin Tan, Colleen Russel, Baixuan Yang, Mengyang Jacky Li, Kexin Wang, Jiadi Xu, Chengyan Chu, Miroslaw Janowski, Tian‐Ming Fu, Piotr Walczak, Yajie Liang   +12 more
wiley   +1 more source