Results 31 to 40 of about 13,141 (192)

In silicoCharacterization of UGT74G1Protein in Stevia rebaudianaBertoni Accession MS007 [PDF]

Journal of Tropical Life Science, 2021
Due to its low-calorie property, Stevia rebaudianais being promoted as an alterna-tive sweetener for diabetic and obese patients. The steady demand in the market for high-quality stevia extracts presents a challenge for the enhanced production of ste ...
Afiqah Ramatullah Khan, Nor Iswani Mokthar, Nor Iswani Mokthar, Afiqah Ramatullah Khan   +3 more
doaj   +1 more source

Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors

Molecules, 2021
Traditional glycosyltransferase (GT) activity assays are not easily configured for rapid detection nor for high throughput screening because they rely on radioactive product isolation, the use of heterogeneous immunoassays or mass spectrometry.
Laurie Engel, Juliano Alves, Jacquelyn Hennek, Said A. Goueli, Hicham Zegzouti   +4 more
doaj   +1 more source

High level in vivo mucin-type glycosylation in Escherichia coli

Microbial Cell Factories, 2018
Background Increasing efforts have been made to assess the potential of Escherichia coli strains for the production of complex recombinant proteins. Since a considerable part of therapeutic proteins are glycoproteins, the lack of the post-translational ...
Phillipp Mueller, Rahul Gauttam, Nadja Raab, René Handrick, Claudia Wahl, Sebastian Leptihn, Michael Zorn, Michaela Kussmaul, Marianne Scheffold, Bernhard Eikmanns, Lothar Elling, Sabine Gaisser   +11 more
doaj   +1 more source

Molecular Cloning and Functional Analysis of IrUGT86A1-like Gene in Medicinal Plant Isodon rubescens (Hemsl.) Hara

Life, 2022
The synthesis of secondary metabolites in plants often includes glycosylation modifications. Often, the final step of constructing plant secondary metabolites is completed by glycosylation transferases, which are also involved in many cell processes.
Conglong Lian, Jinxu Lan, Bao Zhang, Hao Yang, Kaihua Guo, Jingjing Li, Suiqing Chen   +6 more
doaj   +1 more source

Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations

Nature Communications, 2019
UGT76G1 is an UDP-glucose dependent glycosyltransferase and a component of the biosynthesis pathway for the natural sugar substitute steviol glycoside.
Ting Yang, Jinzhu Zhang, Dan Ke, Wenxian Yang, Minghai Tang, Jian Jiang, Guo Cheng, Jianshu Li, Wei Cheng, Yuquan Wei, Qintong Li, James H. Naismith, Xiaofeng Zhu   +12 more
doaj   +1 more source

Two Ginseng UDP-Glycosyltransferases Synthesize Ginsenoside Rg3 and Rd [PDF]

Plant and Cell Physiology, 2014
Ginseng is a medicinal herb that requires cultivation under shade conditions, typically for 4-6 years, before harvesting. The principal components of ginseng are ginsenosides, glycosylated tetracyclic terpenes. Dammarene-type ginsenosides are classified into two groups, protopanaxadiol (PPD) and protopanaxatriol (PPT), based on their hydroxylation ...
Jung, SC Jung, Suk-Chae, Kim, W Kim, Woohyun, Park, SC Park, Sung Chul, Jeong, J Jeong, Jinkil, Park, MK Park, Myung Keun, Lim, S Lim, Soohwan, Lee, Y Lee, Yeon, Im, WT Im, Wan-Taek, Lee, JH Lee, Jun Hyoung, Choi, GT Choi, Gil-Tsu, Kim, SC Kim, Sun Chang   +10 more
openaire   +3 more sources

NleB2 from enteropathogenic Escherichia coli is a novel arginine-glucose transferase effector.

PLoS Pathogens, 2021
During infection, enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) directly manipulate various aspects of host cell function through the translocation of type III secretion system (T3SS) effector proteins directly into the ...
Cristina Giogha, Nichollas E Scott, Tania Wong Fok Lung, Georgina L Pollock, Marina Harper, Ethan D Goddard-Borger, Jaclyn S Pearson, Elizabeth L Hartland   +7 more
doaj   +1 more source

The function of UDP-glycosyltransferases in plants and their possible use in crop protection

Biotechnology Advances, 2023
Glycosyltransferases catalyse the transfer of a glycosyl moiety from a donor to an acceptor. Members of this enzyme class are ubiquitous throughout all kingdoms of life and are involved in the biosynthesis of countless types of glycosides. Family 1 glycosyltransferases, also referred to as uridine diphosphate-dependent glycosyltransferases (UGTs ...
Hani, Gharabli, Valeria, Della Gala, Ditte Hededam, Welner   +2 more
openaire   +2 more sources

UGT76B1 and 41 Additional Arabidopsis UDP-Glycosyltransferases Show No Detectable In Vitro Glycosylation Activity Toward N-Hydroxypipecolic Acid

Life
N-hydroxypipecolic acid (NHP) is a key mobile signal in systemic acquired resistance in plants, and its glycosylation has been proposed to regulate immune signaling. Previous studies have demonstrated that the UDP-glycosyltransferase UGT76B1, known as an
Jiyuan Bao, Taiga Uchiyama, Kazuki Kusunoki, Yuka Shinohara, Yurika Tanigawa, Megumi Watanabe, Nanami Sakata, Hidenori Matsui, Kazuhiro Toyoda, Yuki Ichinose, Yoshiteru Noutoshi   +10 more
doaj   +1 more source

Synthesis and Preliminary Evaluation of Biological Activity of Glycoconjugates Analogues of Acyclic Uridine Derivatives

Molecules, 2018
Herein we present the methodology for obtaining glycosyltransferase inhibitors, analogues of natural enzyme substrates of donor-type: UDP-glucose and UDP-galactose.
Roman Komor, Gabriela Pastuch-Gawolek, Ewelina Krol, Wieslaw Szeja   +3 more
doaj   +1 more source