Results 1 to 10 of about 145,728 (236)

3D-Printing for Analytical Ultracentrifugation [PDF]

PLOS ONE, 2016
Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution.
Desai, Abhiksha, Krynitsky, Jonathan, Pohida, Thomas J., Schuck, Peter, Zhao, Huaying   +4 more
core   +16 more sources

Ultracentrifugation in Biochemistry

Journal of the American Chemical Society, 1959
H. K. Schachman
  +9 more sources

New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles

Frontiers in Bioengineering and Biotechnology, 2021
Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs,
Thanh Huyen Phan, Shiva Kamini Divakarla, Jia Hao Yeo, Qingyu Lei, Priyanka Tharkar, Taisa Nogueira Pansani, Kathryn G. Leslie, Maggie Tong, Victoria A. Coleman, Åsa Jämting, Mar-Dean Du Plessis, Elizabeth J. New, Elizabeth J. New, Bill Kalionis, Philip Demokritou, Hyun-Kyung Woo, Hyun-Kyung Woo, Yoon-Kyoung Cho, Yoon-Kyoung Cho, Wojciech Chrzanowski   +19 more
doaj   +1 more source

Ready-made chromatography columns for extracellular vesicle isolation from plasma [PDF]

Journal of Extracellular Vesicles, 2015
Proteomic studies of circulating vesicles are hampered by difficulties in purifying vesicles from plasma and serum. Isolations are contaminated with high-abundance blood proteins that may mask genuine vesicular-associated proteins and/or simply provide ...
Joanne Louise Welton, Jason Paul Webber, Laur-Alexandru Botos, Michael Jones, Aled Clayton   +4 more
doaj   +1 more source

Metabolomics of small extracellular vesicles derived from isocitrate dehydrogenase 1-mutant HCT116 cells collected by semi-automated size exclusion chromatography

Frontiers in Molecular Biosciences, 2023
Cancer-derived small extracellular vesicles (sEVs) are multifunctional particles with a lipid bilayer structure that are involved in cancer progression, such as malignant proliferation, distant metastasis, and cancer immunity evasion.
Ryosuke Hayasaka, Ryosuke Hayasaka, Sho Tabata, Masako Hasebe, Satsuki Ikeda, Tomoya Hikita, Chitose Oneyama, Chitose Oneyama, Chitose Oneyama, Chitose Oneyama, Jun Yoshitake, Daisuke Onoshima, Kumiko Takahashi, Takahiro Shibata, Koji Uchida, Koji Uchida, Yoshinobu Baba, Yoshinobu Baba, Yoshinobu Baba, Yoshinobu Baba, Tomoyoshi Soga, Tomoyoshi Soga, Masaru Tomita, Masaru Tomita, Akiyoshi Hirayama, Akiyoshi Hirayama, Akiyoshi Hirayama   +26 more
doaj   +1 more source

Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol [PDF]

Journal of Extracellular Vesicles, 2014
Calcifying extracellular vesicles (EVs) released from cells within atherosclerotic plaques have received increased attention for their role in mediating vascular calcification, a major predictor of cardiovascular morbidity and mortality.
Joshua D. Hutcheson, Claudia Goettsch, Tan Pham, Masaya Iwashita, Masanori Aikawa, Sasha A. Singh, Elena Aikawa   +6 more
doaj   +1 more source

The synthesis of extracellular vesicles by the protistan parasite Blastocystis

Frontiers in Cellular and Infection Microbiology, 2022
Blastocystis is a genus of single-celled protist belonging to the stramenopile group. Prior studies have shown that isolates of Blastocystis subtype 7 (ST7) induced higher levels of intestinal epithelial cell damage and gut microbiota dysbiosis in ...
Steven Santino Leonardi, Eileen Yiling Koh, Lei Deng, Chenyuan Huang, Chenyuan Huang, Lingjun Tong, Lingjun Tong, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Kevin Shyong-Wei Tan   +11 more
doaj   +1 more source

Comparison of Different Isolation Methods for Plasma-Derived Extracellular Vesicles in Patients with Hyperlipidemia

Life, 2022
Extracellular vesicles are commonly found in human body fluids and can reflect current physiological conditions of human body and act as biomarkers of disease.
Ke Zhen, Xiaojuan Wei, Zelun Zhi, Shuyan Zhang, Liujuan Cui, Yue Li, Xia Chen, Jing Yao, Hongchao Zhang   +8 more
doaj   +1 more source

A Simple and Effective Method to Concentrate Hepatitis C Virus: Aqueous Two-Phase System Allows Highly Efficient Enrichment of Enveloped Viruses

Viruses, 2022
To investigate the proliferation cycle of a virus, virus-host interaction, and pathogenesis of a virus, virion particles must be concentrated from the media of virus cell culture or the sera of virus-infected patients.
Heesun Kim, Johan Yi, Jinbae Yu, Jaesung Park, Sung Key Jang   +4 more
doaj   +1 more source

Isolation and Characterization of Plasma-Derived Exosomes from the Marine Fish Rock Bream (Oplegnathus fasciatus) by Two Isolation Techniques

Fishes, 2022
Exosomes are important mediators of intercellular communication and modulate many physiological and pathological processes. Knowledge of secretion, content, and biological functions of fish exosomes during pathological infection is still scarce due to ...
Chamilani Nikapitiya, Eriyawala Hewage Thimira Thulshan Jayathilaka, Shan Lakmal Edirisinghe, Dinusha C. Rajapaksha, Withanage Prasadini Wasana, Jayasinghage Nirmani Chathurangika Jayasinghe, Mahanama De Zoysa   +6 more
doaj   +1 more source