Results 1 to 10 of about 174,352 (378)

Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics [PDF]

Nature Communications, 2019
The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in ...
Aikaterini Geladaki, Nina Kočevar Britovšek, Lisa M. Breckels, Tom Smith, Owen L. Vennard, Claire M. Mulvey, Oliver M. Crook, Laurent Gatto, Kathryn S. Lilley   +8 more
openalex   +2 more sources

Residual urinary extracellular vesicles in ultracentrifugation supernatants after hydrostatic filtration dialysis enrichment [PDF]

Journal of Extracellular Vesicles, 2017
Urinary extracellular vesicles (UEVs) appear an ideal source of biomarkers for kidney and urogenital diseases. The majority of protocols designed for their isolation are based on differential centrifugation steps.
Luca Musante, Dorota Tataruch-Weinert, Dontscho Kerjaschki, Michael Henry, Paula Meleady, Harry Holthofer   +5 more
doaj   +2 more sources

Morphological Characterization of Plasma-Derived Nanoparticles Isolated by High-Speed Ultracentrifugation: A Scanning Electron Microscopy Study. [PDF]

Int J Mol Sci
Kungurova LA, Artamonov AA, Grigoryev EA, Aronov AY, Vezo OS, Glushakov RI, Kondratov KA.   +6 more
europepmc   +3 more sources

A Simple and Effective Method to Concentrate Hepatitis C Virus: Aqueous Two-Phase System Allows Highly Efficient Enrichment of Enveloped Viruses

Viruses, 2022
To investigate the proliferation cycle of a virus, virus-host interaction, and pathogenesis of a virus, virion particles must be concentrated from the media of virus cell culture or the sera of virus-infected patients.
Heesun Kim, Johan Yi, Jinbae Yu, Jaesung Park, Sung Key Jang   +4 more
doaj   +1 more source

New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles

Frontiers in Bioengineering and Biotechnology, 2021
Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs,
Thanh Huyen Phan, Shiva Kamini Divakarla, Jia Hao Yeo, Qingyu Lei, Priyanka Tharkar, Taisa Nogueira Pansani, Kathryn G. Leslie, Maggie Tong, Victoria A. Coleman, Åsa Jämting, Mar-Dean Du Plessis, Elizabeth J. New, Elizabeth J. New, Bill Kalionis, Philip Demokritou, Hyun-Kyung Woo, Hyun-Kyung Woo, Yoon-Kyoung Cho, Yoon-Kyoung Cho, Wojciech Chrzanowski   +19 more
doaj   +1 more source

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles

Journal of Extracellular Vesicles, 2022
Small extracellular vesicles (sEVs) provide major promise for advances in cancer diagnostics, prognostics, and therapeutics, ascribed to their distinctive cargo reflective of pathophysiological status, active involvement in intercellular communication ...
Kekoolani S Visan, R. Lobb, S. Ham, L. Lima, C. Palma, Chai Pei Zhi Edna, Li-Ying Wu, Harsha Gowda, K. Datta, G. Hartel, C. Salomon, A. Möller   +11 more
semanticscholar   +1 more source

Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative Luciferase-Vpr packaging-based assay [PDF]

, 2011
The HIV-1 auxiliary protein Vpr and Vpr-fusion proteins can be copackaged with Gag precursor (Pr55Gag) into virions or membrane-enveloped virus-like particles (VLP).
Elisabeth Errazuriz, Eugene Oliver Major, Florence Souquet, Gaëlle Gonzalez, Pascale Coric, Pierre Boulanger, Sandrina DaFonseca, Saw See Hong, Serge Bouaziz, Serge Turcaud   +9 more
core   +13 more sources

High thermoelectric performance of flexible nanocomposite films based on Bi2Te3 nanoplates and carbon nanotubes selected using ultracentrifugation

Scientific Reports, 2023
Thermoelectric generators with flexibility and high performance near 300 K have the potential to be employed in self-supporting power supplies for Internet of Things (IoT) devices.
Tomoyuki Chiba, Hayato Yabuki, M. Takashiri   +2 more
semanticscholar   +1 more source

Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotor

Gene Therapy, 2023
Adeno-associated virus (AAV) vector-based gene therapy is potentially curative for various genetic diseases; however, the development of a scalable purification method for full-genome AAV vectors remains crucial to increase productivity and reduce cost ...
Mikako Wada, N. Uchida, Guillermo Posadas-Herrera, Hiromi Hayashita-Kinoh, Yuji Tsunekawa, Y. Hirai, T. Okada   +6 more
semanticscholar   +1 more source

Isolation and Characterization of Plasma-Derived Exosomes from the Marine Fish Rock Bream (Oplegnathus fasciatus) by Two Isolation Techniques

Fishes, 2022
Exosomes are important mediators of intercellular communication and modulate many physiological and pathological processes. Knowledge of secretion, content, and biological functions of fish exosomes during pathological infection is still scarce due to ...
Chamilani Nikapitiya, Eriyawala Hewage Thimira Thulshan Jayathilaka, Shan Lakmal Edirisinghe, Dinusha C. Rajapaksha, Withanage Prasadini Wasana, Jayasinghage Nirmani Chathurangika Jayasinghe, Mahanama De Zoysa   +6 more
doaj   +1 more source