Results 11 to 20 of about 33,743 (304)

Genetic incorporation of unnatural amino acids into proteins in Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2010
New tools are needed to study the intracellular pathogen Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), to facilitate new drug discovery and vaccine development.
Feng Wang, Scott Robbins, Jiantao Guo, Weijun Shen, Peter G Schultz   +4 more
doaj   +1 more source

Trifluoroselenomethionine: A New Unnatural Amino Acid [PDF]

ChemBioChem, 2016
AbstractTrifluoroselenomethionine (TFSeM), a new unnatural amino acid, was synthesized in seven steps from N‐(tert‐butoxycarbonyl)‐l‐aspartic acid tert‐butyl ester. TFSeM shows enhanced methioninase‐induced cytotoxicity, relative to selenomethionine (SeM), toward HCT‐116 cells derived from human colon cancer.
Eric Block, Squire J. Booker, Sonia Flores‐Penalba, Graham N. George, Sivaji Gundala, Bradley J. Landgraf, Jun Liu, Stephene N. Lodge, M. Jake Pushie, Sharon Rozovsky, Abith Vattekkatte, Rama Yaghi, Huawei Zeng   +12 more
openaire   +3 more sources

Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity

Results in Chemistry, 2023
Protease specificity depends on the three-dimensional structure and sequence of the protease, and each protease plays a different role. Chymotrypsin and subtilisin have similar specificity for recognizing aromatic and hydrophobic amino acid residues ...
Yuuki Yamawaki, Tamaki Kato
doaj   +1 more source

Synthesis of Isothiocyanates Using DMT/NMM/TsO⁻ as a New Desulfurization Reagent

Molecules, 2021
Thirty-three alkyl and aryl isothiocyanates, as well as isothiocyanate derivatives from esters of coded amino acids and from esters of unnatural amino acids (6-aminocaproic, 4-(aminomethyl)benzoic, and tranexamic acids), were synthesized with ...
Łukasz Janczewski, Dorota Kręgiel, Beata Kolesińska   +2 more
doaj   +1 more source

Electrochemical Synthesis of Unnatural Amino Acids Embedding 5- and 6‑Membered Heteroaromatics [PDF]

ACS Omega
Elena Bombonato, Valerio Fasano, Daniel Pecorari, Luca Fornasari, Francesco Castagnini, Massimo Marcaccio, Paolo Ronchi   +6 more
doaj   +2 more sources

Photoinduced β-fragmentation of aliphatic alcohol derivatives for forging C–C bonds

Nature Communications, 2022
Transformations of alcohols, which are ubiquitous in chemistry and are native functionalities in many natural products and bioactive molecules, are cornerstones of organic synthesis.
Yiman Gao, Jie Liu, Cong Wei, Yan Li, Kui Zhang, Liangliang Song, Lingchao Cai   +6 more
doaj   +1 more source

Enzymatic Screening of β-Amyloid Precursor Protein-Based Substrates

Compounds, 2023
We performed an enzymatic screening of synthetic peptides based on β-amyloid precursor protein substrates. The template peptide sequence was a decapeptide derived from our previous screening study, which determined several effective unnatural amino acids.
Reo Yamada, Masaki Midorikawa, Ayu Asai, Norimasa Takasu, Ren Fujii, Taeko Kakizawa   +5 more
doaj   +1 more source

Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids

Nature Communications, 2023
The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan, Guobang Li, Ruxin Liu, Jiawei Guo, Yunjie Liu, Mingyu Liu, Xingwang Zhang, Luping Chi, Kangwei Xu, Ruibo Wu, Yuzhong Zhang, Yuezhong Li, Xiang Gao, Shengying Li   +13 more
doaj   +1 more source

An engineered Tetrahymena tRNA(Gln) for in vivo incorporation of unnatural amino acids into proteins by nonsense suppression [PDF]

, 1996
A new tRNA, THG73, has been designed and evaluated as a vehicle for incorporating unnatural amino acids site-specifically into proteins expressed in vivo using the stop codon suppression technique.
Abelson, John N., Dougherty, Dennis A., Du, Fangyong, Kearney, Patrick C., Lester, Henry A., Nowak, Mark W., Saks, Margaret E., Sampson, Jeffrey R.   +7 more
core   +1 more source

Development of the force field for cyclosporine A

Biophysics and Physicobiology, 2022
Membrane permeability of cyclic peptides is an important factor in drug design. To investigate the membrane permeability of cyclic peptides using molecular dynamics (MD) simulations, the accurate force fields for unnatural amino acids present in the ...
Tsutomu Yamane, Toru Ekimoto, Mitsunori Ikeguchi   +2 more
doaj   +1 more source