Enzymatic aminoacylation of tRNA with unnatural amino acids [PDF]
Proceedings of the National Academy of Sciences, 2006 The biochemical flexibility of the cellular translation apparatus offers, in principle, a simple route to the synthesis of drug-like modified peptides and novel biopolymers. However, only ≈75 unnatural building blocks are known to be fully compatible with enzymatic tRNA acylation and subsequent ribosomal synthesis of modified ...
Matthew C T, Hartman +2 more
openaire +2 more sources
Expanding the genetic code of Mus musculus
Nature Communications, 2017 Expanding the genetic code with unnatural amino acids in model organisms is a powerful tool for investigatingin vivocellular proteins and processes. Here the authors re-engineer the amber stop codon in laboratory mice to allow site-specific incorporation
Songmi Han +5 more
doaj +1 more source
Xenogeneic, extracorporeal liver perfusion in primates improves the ratio of branched-chain amino acids to aromatic amino acids (Fischer's ratio) [PDF]
, 1999 In fulminant hepatic failure (FHF), the development of hepatic encephalopathy is associated with grossly abnormal concentrations of plasma amino acids (PAA).
Puhl, G. +7 more
core +1 more source
Antimicrobial Activity of Amino Acid Analogues and Their Derivatives
Proceedings, 2017 Libraries of compounds containing unnatural amino acids or amino acid analogues were prepared from inexpensive substrates using sustainable processes [1–3]. [...]
Dácil Hernández +4 more
doaj +1 more source
Protein engineering with unnatural amino acids
Current Opinion in Structural Biology, 2013 Protein engineering has become an extensively used tool in many fields, allowing us to probe protein function, characterize proteins using a range of biophysical techniques, chemically modify proteins and improve protein function for medical and industrial applications.
Zhang, William +2 more
openaire +3 more sources
Copper-catalysed azide–alkyne cycloaddition on live M13 bacteriophage for expanding the molecular diversity of phage-displayed peptide libraries [PDF]
RSC Chemical BiologyPhage display is a powerful platform for ligand evolution, but conventional phage display libraries are confined to the twenty canonical amino acids, greatly limiting the chemical space that these libraries can be used to explore.
Olabode Dawodu +4 more
doaj +1 more source
Towards Enantiomerically Pure Unnatural α-Amino Acids via Photoredox Catalytic 1,4-Additions to a Chiral Dehydroalanine [PDF]
, 2022 [Image: see text] Chemo- and diastereoselective 1,4-conjugate additions of anionic and radical C-nucleophiles to a chiral bicyclic dehydroalanine (Dha) are described.
Corzana, Francisco [0000-0001-5597-8127] +20 more
core +1 more source
Minimal genetically encoded tags for fluorescent protein labeling in living neurons
Nature Communications, 2022 Site-specific labelling of proteins can be performed with unnatural amino acids combined with bioorthogonal click chemistry. Here the authors establish this in living neurons, using neurofilament light chain; they show combination with CRISPR/Cas9 ...
Aleksandra Arsić +4 more
doaj +1 more source
, 2022 Proteinogenic amino acids are natural nutrients ingested daily from standard foods. Commercially manufactured amino acids are added to a wide range of nutritional products, including dietary supplements and regular foods.
Arashida, Naoko +9 more
core +1 more source
Metal-responsive regulation of enzyme catalysis using genetically encoded chemical switches
Nature Communications, 2022 Dynamic control over protein function is a central challenge in synthetic biology. Here the authors present an integrated computational and experimental workflow for engineering reversible protein switches; metal-chelating unnatural amino acids ...
Yasmine S. Zubi +7 more
doaj +1 more source