Results 21 to 30 of about 1,288,650 (300)
Enzymatic Screening of β-Amyloid Precursor Protein-Based Substrates
Compounds, 2023 We performed an enzymatic screening of synthetic peptides based on β-amyloid precursor protein substrates. The template peptide sequence was a decapeptide derived from our previous screening study, which determined several effective unnatural amino acids.
Reo Yamada +5 more
doaj +1 more source
Nature Communications, 2023 The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan +13 more
doaj +1 more source
Structure-Function Studies of Nicotinic Acetylcholine Receptors Using Unnatural Amino Acids [PDF]
, 2012 This dissertation primarily describes structure-function studies of the nicotinic acetylcholine receptors (nAChRs). These studies use a combination of unnatural amino acid mutagenesis and electrophysiology to determine the specific molecular interactions
Puskar, Nyssa Leigh
core +1 more source
In Vivo Incorporation of Multiple Unnatural Amino Acids [PDF]
, 2009 Unnatural amino acid (UAA) incorporation is an invaluable technique that is seeing increased use. THG73 is an amber suppressor tRNA used to incorporate > 100 residues at the UAG, amber stop codon, in Xenopus oocytes.
Rodriguez, Erik Ali
core +1 more source
Development of the force field for cyclosporine A
Biophysics and Physicobiology, 2022 Membrane permeability of cyclic peptides is an important factor in drug design. To investigate the membrane permeability of cyclic peptides using molecular dynamics (MD) simulations, the accurate force fields for unnatural amino acids present in the ...
Tsutomu Yamane +2 more
doaj +1 more source
NMR Studies of Large Protein Dynamics Using Unnatural Amino Acids
Chinese Journal of Magnetic Resonance, 2021 Nuclear magnetic resonance (NMR) is a major method used to study protein structure at atomic resolution. Besides presenting the high-resolution structure, NMR facilitates studies on protein dynamics near physiological conditions that are intimately ...
Chao-wei SHI, Pan SHI, Chang-lin TIAN
doaj +1 more source
Selective quantitative N-functionalization of unprotected α-amino acids using NHC-Ir(III) catalyst
STAR Protocols, 2023 Summary: Unnatural amino acids are valuable building blocks with numerous applications. Here, we present a quantitative technique for accessing mono-N-functionalized amino acids directly from unprotected substrates using alcohols as alkylating agents and
Beatriz Saavedra +3 more
doaj +1 more source
Frontiers in Chemistry, 2019 Unnatural amino acids have tremendously expanded the folding possibilities of peptides and peptide mimics. While α,α-disubstituted and β-amino acids are widely studied, γ-derivatives have been less exploited.
Francesco Oliva +5 more
doaj +1 more source
Biochemistry and Biophysics Reports, 2019 Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules.
Leonhard Jakob +2 more
doaj +1 more source
Novel Crown Ether Amino Acids as Fluorescent Reporters for Metal Ions
Molecules, 2023 Unnatural amino acids with enhanced properties, such as increased complexing ability and luminescence, are considered to be highly attractive building blocks for bioinspired frameworks, such as probes for biomolecule dynamics, sensitive fluorescent ...
Patrícia M. R. Batista +3 more
doaj +1 more source