Results 31 to 40 of about 33,743 (304)
Recent advances in the synthesis and application of fluorescent α-amino acids [PDF]
, 2016 Fluorescence spectroscopy has become a powerful technique for probing a range of complex biological processes including enzyme mechanisms and protein-protein interactions. While the application of this technique uses a number of strategies, many of these
Harkiss, Alexander, Sutherland, Andrew
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Protein engineering with unnatural amino acids
Current Opinion in Structural Biology, 2013 Protein engineering has become an extensively used tool in many fields, allowing us to probe protein function, characterize proteins using a range of biophysical techniques, chemically modify proteins and improve protein function for medical and industrial applications.
Zhang, William +2 more
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Synthesis of complex unnatural fluorine-containing amino acids [PDF]
Journal of Fluorine Chemistry, 2020 The area of fluorinated amino acid synthesis has seen rapid growth over the past decade. As reports of singly fluorinated natural amino acid derivatives have grown, researchers have turned their attention to develop methodology to access complex proteinogenic examples.
William D.G. Brittain +2 more
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A Gating Charge Transfer Center in Voltage Sensors [PDF]
, 2010 Voltage sensors regulate the conformations of voltage-dependent ion channels and enzymes. Their nearly switchlike response as a function of membrane voltage comes from the movement of positively charged amino acids, arginine or lysine, across the ...
Dougherty, Dennis A. +4 more
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Minimal genetically encoded tags for fluorescent protein labeling in living neurons
Nature Communications, 2022 Site-specific labelling of proteins can be performed with unnatural amino acids combined with bioorthogonal click chemistry. Here the authors establish this in living neurons, using neurofilament light chain; they show combination with CRISPR/Cas9 ...
Aleksandra Arsić +4 more
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Protein-based materials, toward a new level of structural control [PDF]
, 2001 Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials.
Tirrell, David A., van Hest, Jan C. M.
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Electrostatic Contributions of Aromatic Residues in the Local Anesthetic Receptor of Voltage-Gated Sodium Channels [PDF]
, 2008 Antiarrhythmics, anticonvulsants, and local anesthetics target voltage-gated sodium channels, decreasing excitability of nerve and muscle cells. Channel inhibition by members of this family of cationic, hydrophobic drugs relies on the presence of highly ...
Ahern, Christopher A. +3 more
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Metal-responsive regulation of enzyme catalysis using genetically encoded chemical switches
Nature Communications, 2022 Dynamic control over protein function is a central challenge in synthetic biology. Here the authors present an integrated computational and experimental workflow for engineering reversible protein switches; metal-chelating unnatural amino acids ...
Yasmine S. Zubi +7 more
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Hachimoji DNA and RNA: A genetic system with eight building blocks [PDF]
, 2019 Reported here are DNA and RNA-like systems built from eight (hachi-) nucleotide letters (-moji) that form four orthogonal pairs. This synthetic genetic biopolymer meets the structural requirements needed to support Darwinism, including a polyelectrolyte ...
Bates, Alison M. +15 more
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Site-specific incorporation of phosphotyrosine using an expanded genetic code. [PDF]
, 2017 Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation.
Allison Wong +36 more
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