Uracil-DNA glycosylase efficiency is modulated by substrate rigidity [PDF]
Scientific Reports, 2023 Uracil DNA-glycosylase (UNG) is a DNA repair enzyme that removes the highly mutagenic uracil lesion from DNA using a base flipping mechanism. Although this enzyme has evolved to remove uracil from diverse sequence contexts, UNG excision efficiency ...
Paul B. Orndorff +8 more
doaj +5 more sources
Crystal structure of mimivirus uracil-DNA glycosylase. [PDF]
PLoS ONE, 2017 Cytosine deamination induced by stresses or enzymatic catalysis converts deoxycytidine into deoxyuridine, thereby introducing a G to A mutation after DNA replication.
Eunju Kwon +3 more
doaj +6 more sources
Computational Study on DNA Repair: The Roles of Electrostatic Interactions Between Uracil-DNA Glycosylase (UDG) and DNA [PDF]
Frontiers in Molecular Biosciences, 2021 Uracil-DNA glycosylase (UDG) is one of the most important base excision repair (BER) enzymes involved in the repair of uracil-induced DNA lesion by removing uracil from the damaged DNA. Uracil in DNA may occur due to cytosine deamination or deoxy uridine
Yixin Xie +5 more
doaj +3 more sources
A New Class of Uracil–DNA Glycosylase Inhibitors Active against Human and Vaccinia Virus Enzyme [PDF]
Molecules, 2021 Uracil–DNA glycosylases are enzymes that excise uracil bases appearing in DNA as a result of cytosine deamination or accidental dUMP incorporation from the dUTP pool. The activity of Family 1 uracil–DNA glycosylase (UNG) activity limits the efficiency of
Inga R. Grin +10 more
doaj +3 more sources
Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase [PDF]
Nature CommunicationsDNA base editing technologies predominantly utilize engineered deaminases, limiting their ability to edit thymine and guanine directly. In this study, we successfully achieve base editing of both cytidine and thymine by leveraging the translesion DNA ...
Zongyi Yi +10 more
doaj +3 more sources
Highly Sensitive Detection of Uracil-DNA Glycosylase Activity Based on Self-Initiating Multiple Rolling Circle Amplification. [PDF]
ACS Omega, 2019 Sensitive detection of uracil-DNA glycosylase (UDG) activity is very important in the study of many fundamental biochemical processes and clinical applications. Here, we develop a novel assay for the detection of UDG activity by using the self-initiating
Dong L +5 more
europepmc +3 more sources
Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity [PDF]
Open Biology, 2021 Base excision repair is one of the important DNA repair mechanisms in cells. The fundamental role in this complex process is played by DNA glycosylases.
Anna Ligasová +4 more
doaj +2 more sources
Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening [PDF]
Biochemistry and Biophysics Reports, 2021 Uracil DNA glycosylases are an important class of enzymes that hydrolyze the N-glycosidic bond between the uracil base and the deoxyribose sugar to initiate uracil excision repair.
Avani Mehta +4 more
doaj +2 more sources
Repair of APOBEC3G-Mutated Retroviral DNA In Vivo Is Facilitated by the Host Enzyme Uracil DNA Glycosylase 2. [PDF]
J Virol, 2021 While APOBEC3-mediated mutation of retroviruses is well-established, what role the host base excision repair enzymes play in correcting these mutations is not clear. This question is especially difficult to address in vivo. ABSTRACT Apolipoprotein B mRNA
Salas-Briceno K, Ross SR.
europepmc +2 more sources
Inhibition of Human Uracil DNA Glycosylase Sensitizes a Large Fraction of Colorectal Cancer Cells to 5-Fluorodeoxyuridine and Raltitrexed but Not Fluorouracil. [PDF]
Mol Pharmacol, 2021 Previous short-hairpin RNA knockdown studies have established that depletion of human uracil DNA glycosylase (hUNG) sensitizes some cell lines to 5-fluorodeoxyuridine (FdU).
Christenson ES +8 more
europepmc +2 more sources