Results 201 to 210 of about 24,501 (233)

Complexes of the uracil-DNA glycosylase inhibitor protein, Ugi, with Mycobacterium smegmatis and Mycobacterium tuberculosis uracil-DNA glycosylases

Microbiology, 2003
Uracil, a promutagenic base, appears in DNA either by deamination of cytosine or by incorporation of dUMP by DNA polymerases. This unconventional base in DNA is removed by uracil-DNA glycosylase (UDG). Interestingly, a bacteriophage-encoded short polypeptide, UDG inhibitor (Ugi), specifically inhibits UDGs by forming a tight complex.
Acharya, Narottam, Kumar, Pradeep, Varshney, Umesh   +2 more
openaire   +2 more sources

URACIL-DNA GLYCOSYLASE MUTANTS ARE MUTATORS

1978
ABSTRACT Spontaneous mutation to nalidixic acid or to rifampicin resistance is increased 5-fold in uracil-DNA glycosylase ( ung ) mutants of E. coli. By trpA reversion analysis we observed a 15-fold increase in the frequency of spontaneous G:C→A:T transition mutations while other transition and transversion frequencies were not affected.
Bruce K. Duncan, Bernard Weiss
openaire   +1 more source

Mutational Analysis of the Uracil DNA Glycosylase Inhibitor Protein and Its Interaction with Escherichia coli Uracil DNA Glycosylase

Journal of Molecular Biology, 2002
Uracil DNA glycosylase inhibitor (Ugi), a protein of 9.4 kDa consists of a five-stranded antiparallel beta sheet flanked on either side by single alpha helices, forms an exclusive complex with uracil DNA glycosylases (UDGs) that is stable in 8M urea. We report on the mutational analysis of various structural elements in Ugi, two of which (hydrophobic ...
Acharya, Narottam, Roy, Sudipta, Varshney, Umesh   +2 more
openaire   +3 more sources

Normal uracil-DNA glycosylase activity in Bloom's syndrome cells

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1989
Cells from patients with Bloom's syndrome, a rare human disease with autosomal recessive mode of inheritance, exhibit cytological abnormalities involving DNA metabolism. Bloom's syndrome is characterized by a greatly increased cancer frequency which may reflect a specific defect in DNA repair and replication. Evidence has recently been presented of the
J A, Vilpo, L M, Vilpo
openaire   +2 more sources

Catalytic single-molecule Förster resonance energy transfer biosensor for uracil-DNA glycosylase detection and cellular imaging.

Biosensors & bioelectronics, 2022
Qian Zhang, Chen-chen Li, Fei Ma, Xiliang Luo, Chun-yang Zhang   +4 more
semanticscholar   +1 more source

Lessons learned from structural results on uracil-DNA glycosylase

Mutation Research/DNA Repair, 2000
Uracil-DNA glycosylase (UDG) functions as a sentry guarding against uracil in DNA. UDG initiates DNA base excision repair (BER) by hydrolyzing the uracil base from the deoxyribose. As one of the best studied DNA glycosylases, a coherent and complete functional mechanism is emerging that combines structural and biochemical results.
S S, Parikh, C D, Putnam, J A, Tainer
openaire   +2 more sources

Modified substrates as probes for studying uracil-DNA glycosylase

Gene, 1995
In order to study the mechanism of action of uracil-DNA glycosylase (UDG) from human placenta, single-stranded (ss) and double-stranded (ds) oligodeoxyribonucleotides (oligos), containing deoxyuridine (dU) and a wide variety of their analogs were used. It was shown that UDG has a twofold preference for ss oligos over ds oligos and a twofold preference ...
E A, Kubareva, E M, Volkov, N L, Vinogradova, I A, Kanevsky, T S, Oretskaya, S A, Kuznetsova, M G, Brevnov, E S, Gromova, G A, Nevinsky, Z A, Shabarova   +9 more
openaire   +2 more sources

Analysis of uracil DNA glycosylase in human colorectal cancer

International Journal of Oncology, 2001
Uracil DNA glycosylase (UDG) is responsible for the removal of uracil present in DNA after cytosine deamination or misincorporation during replication. Colorectal cancer is widely treated with 5-FU, which leads to thymidylate synthase inhibition; this accounts for increased dUTP intracellular pools and subsequent uracil incorporation into DNA.
C, Dusseau, G I, Murray, R A, Keenan, T, O'Kelly, H E, Krokan, H L, McLeod   +5 more
openaire   +2 more sources

Identification of a novel bifunctional uracil DNA glycosylase from Thermococcus barophilus Ch5

Applied Microbiology and Biotechnology, 2021
Likui Zhang, Donghao Jiang, Qi Gan, Haoqiang Shi, Li Miao, Y. Gong, P. Oger   +6 more
semanticscholar   +1 more source

Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions.

Gene, 1990
Mary C. Longo, M. Berninger, J. Hartley
semanticscholar   +1 more source