Results 71 to 80 of about 22,001 (241)

An Update: Enzymatic Synthesis for Industrial Applications

Angewandte Chemie International Edition, EarlyView.
Biocatalysis has become a sustainable and cost‐competitive alternative to established chemical synthesis, enabling the enzyme‐based production of not only commodity chemicals but (non‐natural) amino acids, (rare) sugars, as well as synthetic nucleotides.
Thomas Bayer, Shuke Wu, Radka Snajdrova, Kai Baldenius, Uwe T. Bornscheuer   +4 more
wiley   +1 more source

Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA [PDF]

, 2006
In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion.
Ashkenas, Belinsky, Borst, Cairns, Cimino, Cimino, Clark, Cottrell, Cottrell, Cottrell, Das, Dimo Dietrich, Dulaimi, Dwyer, Eads, Fujiwara, Fukuzawa, Herman, Herman, Hoque, Ichikawa, Juergen Distler, Liu, Lofton-Day, Longo, Melnikov, Muller, Niederhauser, Olek, Pang, Pennings, Pruvost, Reimo Tetzner, Rys, Sabbioni, Udaykumar, Verma, Walder   +37 more
core   +2 more sources

Cloning and Assembly of PCR Products Using Modified Primers and DNA Repair Enzymes

BioTechniques, 1997
We present a method for the creation of ligatable 3′overhangs by the incorporation of a modified base, uracil, at a specific position in the PCR primer and subsequent treatment with the DNA-modifying enzyme uracil DNA glycosylase and then either T4 ...
David E. Watson, George N. Bennett
doaj   +1 more source

Repair of Hypoxanthine in DNA Revealed by DNA Glycosylases and Endonucleases From Hyperthermophilic Archaea

Frontiers in Microbiology, 2021
Since hyperthermophilic Archaea (HA) thrive in high-temperature environments, which accelerate the rates of deamination of base in DNA, their genomic stability is facing a severe challenge.
Tan Lin, Likui Zhang, Likui Zhang, Mai Wu, Donghao Jiang, Zheng Li, Zhihui Yang   +6 more
doaj   +1 more source

Orchestrated desaturation reprogramming from stearoyl‐CoA desaturase to fatty acid desaturase 2 in cancer epithelial‐mesenchymal transition and metastasis

Cancer Communications, Volume 45, Issue 3, Page 245-280, March 2025.
Abstract Background Adaptative desaturation in fatty acid (FA) is an emerging hallmark of cancer metabolic plasticity. Desaturases such as stearoyl‐CoA desaturase (SCD) and fatty acid desaturase 2 (FADS2) have been implicated in multiple cancers, and their dominant and compensatory effects have recently been highlighted.
Zhicong Chen, Yanqing Gong, Fukai Chen, Hyeon Jeong Lee, Jinqin Qian, Jing Zhao, Wenpeng Zhang, Yamin Li, Yihui Zhou, Qiaobing Xu, Yu Xia, Liqun Zhou, Ji‐Xin Cheng   +12 more
wiley   +1 more source

Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity. [PDF]

, 2006
The archaeal/eukaryotic proliferating cell nuclear antigen (PCNA) toroidal clamp interacts with a host of DNA modifying enzymes, providing a stable anchorage and enhancing their respective processivities.
Andrew S. Doré, Antony W. Oliver, Brunger, Bunting, CCP4, Chang, Chapados, Dalrymple, DeLano, Dianova, Dionne, Dionne, Emsley, Friedrich-Heineken, Gulbis, Helt, Hingorani, Hosfield, Kelman, Kong, Kontopidis, Krishna, Krokan, Laurence H. Pearl, Leslie, Mairi L. Kilkenny, Matsumiya, Matsumoto, Murante, Murshudov, Oyama, Parker, Parrilla-Castellar, Pearl, Roberts, S. Mark Roe, Sakurai, Sarah A. Jones, Shamoo, Shen, Smirnova, Stephen D. Bell, Storoni, Tom, Toueille, Tsuchimoto, Venclovas, Volkmer, Wang   +48 more
core   +4 more sources

The ‘very moment’ when UDG recognizes a flipped-out uracil base in dsDNA

Scientific Reports
Uracil-DNA glycosylase (UDG) is the first enzyme in the base-excision repair (BER) pathway, acting on uracil bases in DNA. How UDG finds its targets has not been conclusively resolved yet.
Vinnarasi Saravanan, Nessim Raouraoua, Guillaume Brysbaert, Stefano Giordano, Marc F. Lensink, Fabrizio Cleri, Ralf Blossey   +6 more
doaj   +1 more source

Evolutionary Origins of DNA Repair Pathways: Role of Oxygen Catastrophe in the Emergence of DNA Glycosylases

Cells, 2021
It was proposed that the last universal common ancestor (LUCA) evolved under high temperatures in an oxygen-free environment, similar to those found in deep-sea vents and on volcanic slopes.
Paulina Prorok, Inga R. Grin, Bakhyt T. Matkarimov, Alexander A. Ishchenko, Jacques Laval, Dmitry O. Zharkov, Murat Saparbaev   +6 more
doaj   +1 more source

Organocatalytic Switches of DNA Glycosylase OGG1 Catalyze a Highly Efficient AP‐Lyase Function

Chemistry – A European Journal, EarlyView.
Organocatalytic switches are small molecules that combine active site affinity and a nitrogen‐centered base for catalysis, effectively removing the rate‐determining step of the OGG1 biochemical cascade. Here, we identify a minimal structure and modulate the active nitrogen pKa, enabling the catalysis of ultrafast AP site incisions in a range of pH ...
Mario Kehler, Kaixin Zhou, Aurino M. Kemas, Alicia del Prado, Emma Scaletti Hutchinson, Elinor Hesslefors Nairn, Marek Varga, Yvonne Plattner, Yi Zhong, Oryn Purewal‐Sidhu, James Haslam, Elisée Wiita, Heather Gildie, Karolina Singerova, Zuzanna Szaruga, Ingrid Almlöf, Femke M. Hormann, Kang‐Cheng Liu, Olov Wallner, Florian Ortis, Evert J. Homan, Opher Gileadi, Sean G. Rudd, Pål Stenmark, Miguel de Vega, Thomas Helleday, Nicholas D. D'Arcy‐Evans, Volker M. Lauschke, Maurice Michel   +28 more
wiley   +1 more source

Determination of Methylation Site of DNA-Methyltransferase NlaX by a Hybrid Method

BioTechniques, 2002
Using a new method based on a combination of bisulfite reaction, the repair enzyme uracil-DNA glycosylase, and synthetic oligodeoxyribonucleotides, the methylation site of DNA-methyltransferase NlaX (M·NlaX) from Neisseria lactamica was established to be
E.A. Kubareva, J. Walter, A.S. Karyagina, O.V. Vorob'eva, P.C.K. Lau, T. Trautner   +5 more
doaj   +1 more source