Results 131 to 140 of about 86,414 (313)
Transgenic selection and underlying mechanisms in apicomplexan parasites
The FEBS Journal, EarlyView.Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju +2 more
wiley +1 more source
Analysis of the sensitivity of different fungal species to uracil [PDF]
, 2016 The use of auxotrophies as a tool for genetic modification of microbial strains is widely employed both in fundamental (e.g., molecular characterization of genes based on reverse genetics approaches) and applied research (e.g., maintenance of plasmids ...
Silva, R. +2 more
core
Fragmentation of uracil after electron capture by doubly charged ions
, 2015 Single electron capture from molecular uracil (C4H4N2O2) by doubly-charged He2+, C2+, and O2+ atomic ions followed by the fragmentation of uracil ions has been studied using time-of-flight mass ...
Michael N. Panov +4 more
core +1 more source
The FEBS Journal, EarlyView.Blocking protein quality control degradation by deleting the E3 ligases Ubr1 or San1 stabilizes many DHFR insertion and deletion variants that are otherwise partially unfolded. These hypomorphic variants regain folding at lower temperatures or upon methotrexate binding, revealing that PQC degradation can remove proteins that are still functionally ...
Sven Larsen‐Ledet +6 more
wiley +1 more source
Replication Protein A (RPA) Targeting of Uracil DNA Glycosylase (UNG2)
, 2021 Replication Protein A (RPA) is a single stranded DNA binding protein which stabilizes ssDNA for replication and repair. One function of RPA is to bind the DNA repair enzyme uracil DNA glycosylase (UNG2) and direct its activity towards ssDNA dsDNA ...
Weiser, Brian P, Chen, Derek
core
Emerging experimental and bioinformatic approaches in RNA interference‐based pest control research
Insect Molecular Biology, EarlyView.Double‐stranded RNA (dsRNA) visualization, RNA‐induced silencing complex (RISC)‐bound sRNA‐seq and RNA degradomics allow investigating dsRNA uptake and processing, guide small interfering RNA selection and RISC‐mediated target mRNA cleavages. Transcriptomic and proteomic profiles of genome‐wide responses to dsRNA, including off‐target and compensatory ...
Doga Cedden
wiley +1 more source
The Development of Exogenous Anticonvulsants and Endogenous Uracil-Based Antiepileptic Agents
, 2011 Epilepsy is a common neurological disorder for which the development of new and improved therapies is essential. Thus, the central theme of this thesis pertains to the design and synthesis of putative antiepileptic drugs.
Ward, Sarah
core
Why does uridine supplementation properly rescues the growth of Ashbya gossypii uracil auxotrophs, but not uracil? [PDF]
, 2016 The use of auxotrophies as a tool for genetic modification of microbial strains is widely employed in microbiology laboratories. However, there are some recognized problems associated with their use, such as the fact that it is unclear how much ...
Silva, Rui +2 more
core
Immunology &Cell Biology, EarlyView.VitBAg antigen presentation is promoted through a cytosolic pathway that reaches ligand‐receptive MR1 to present to MAIT cells, like the riboflavin transport pathway. Riboflavin solute carrier transporters can promote this MR1 presentation, but they are not essential for this role, suggesting redundant pathways to uptake VitBAg.
Sebastian Cruz‐Gomez +10 more
wiley +1 more source
International Dermoscopy Society consensus recommendations for the management of lentigo maligna
Journal of the European Academy of Dermatology and Venereology, EarlyView.Lentigo maligna (LM) poses diagnostic and therapeutic challenges amid rising incidence and limited high‐quality evidence. This international expert consensus provides pragmatic, evidence‐based and expertise‐informed guidance on LM diagnosis and management.
Ana‐Maria Forsea +52 more
wiley +1 more source