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Enzymic determination of uracil nucleotides in tissues
Analytical Biochemistry, 1970Abstract An enzymic assay for the sequential determination of UDP-glucose, UDP-galactose, UTP, UDP, and 5′-UMP in tissues is described. Two moles of NAD are reduced per mole of uracil nucleotide by use of UDPG dehydrogenase as indicator enzyme. The procedure is highly specific for the measurement of uridine 5′-phosphates.
Dietrich Keppler
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Effects of galactose on human leukocyte uracil nucleotides
International Journal of Biochemistry & Cell Biology, 1975Abstract 1. 1. Uracil nucleotide contents of human leukocytes were determined by enzymatic analysis. The labelling pattern of uracil nucleotides was studied after incubation of the cells in the presence of [ 14 C]uridine followed by separation of the labelled nucleotides by paper chromatography. 2. 2. A depression of UTP, UDP, and UDP-glucose
Johannes Förster, Dietrich Keppler
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Glomerular uracil nucleotide synthesis
American Journal of Physiology-Renal Physiology, 1988The biosynthesis of basement membrane material requires the sugar derivatives of uridine 5'-triphosphate (UTP) for protein glycosylation. Uridine and orotate utilization for the biosynthesis of uracil ribonucleotides was studied in isolated rat glomeruli incubated in vitro. At a 1 microM concentration total orotate utilization was 9.6 +/- 1.8 pmol.min-
P, Cortes, F, Dumler, N W, Levin
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Journal of the Chemical Society Chemical Communications, 1984
Unprotected uracil nucleotides and nucleosides are converted into 5-formyluracil derivatives using a palladium(II) coupling reaction followed by oxidation of the intermediate styryl derivative.
Joon S. Park +4 more
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Unprotected uracil nucleotides and nucleosides are converted into 5-formyluracil derivatives using a palladium(II) coupling reaction followed by oxidation of the intermediate styryl derivative.
Joon S. Park +4 more
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Acidity of a Nucleotide Base: Uracil
The Journal of Physical Chemistry A, 2004Experiment and calculations are used to show that the gas-phase acidity of uracil is comparable to that of HCl. The gas-phase acidity of uracil (denoted here by U) was bracketed by proton-transfer measurements involving Uand various reference acids (denoted here by A) of known gas-phase acidity.
Thomas M. Miller +3 more
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Stimulation of mouse cultured sympathetic neurons by uracil but not adenine nucleotides
Neuroscience, 2001Cultured neurons from the paravertebral sympathetic chain of rats possess excitatory P2X as well as excitatory uracil nucleotide-sensitive P2Y receptors. Preliminary observations had indicated that the analogous neurons of mice lacked P2X receptors. This difference was now investigated.
W Nörenberg +1 more
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P2 Receptors Activated by Uracil Nucleotides - An Update
Current Medicinal Chemistry, 2006Pyrimidine nucleotides, including UTP, UDP and UDP-glucose, are important signaling molecules which activate G protein-coupled membrane receptors (GPCRs) of the P2Y family. Four distinct pyrimidine nucleotide-sensitive P2Y receptor subtypes have been cloned, P2Y2, P2Y4, P2Y6 and P2Y14. P2Y2 and P2Y4 receptors are activated by UTP (the P2Y2, and the rat
Andreas, Brunschweiger +1 more
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Potential signalling roles for utp and udp: sources, regulation and release of uracil nucleotides
Trends in Pharmacological Sciences, 1997Increasing evidence for receptors for uracil nucleotides has focused interest on specific signalling mechanisms involving UTP and UDP. At least three metabotropic P2 receptors are stimulated by uracil nucleotides with equal or greater potency than by adenine nucleotides, and there might be ionotropic receptors as well.
Christopher M Anderson +1 more
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