Results 201 to 210 of about 98,911 (236)

Phosphorylation of uridine and cytidine by uridine–cytidine kinase

Journal of Biotechnology, 2014
Uridine 5'-monophosphate (5'-UMP) and cytidine 5'-monophosphate (5'-CMP) were biosynthesized by recombinant uridine-cytidine kinase (UCK) and acetate kinase (ACK). The ack and uck genes from Escherichia coli K12 and the uck1, uck2 and ack genes from Lactobacillus bulgaricus ATCC 11842 were cloned and inserted into the plasmid pET-28a.
Yahui Qian, Qingbao Ding, Yanyu Li, Zhi Zou, Bingkun Yan, Ling Ou   +5 more
openaire   +3 more sources

Salvage of uridine in the mouse

Biochemical Pharmacology, 1985
Salvage of circulating nucleosides provides an alternative to de novo synthesis of nucleotides and may modify response to antimetabolites. We have investigated treatment with uridine phosphorylase as a means of inhibiting salvage of uridine in vivo. Examination of the metabolism of intravenous [3H] uridine in mice revealed that 30-40% was salvaged by ...
Henderson Jf, James D. Moyer
openaire   +3 more sources

Effects of uridine on kindling

Epilepsy & Behavior, 2008
The anticonvulsant effect of the nucleoside uridine has been studied for several decades with controversial results. One of its attractive properties is that as a natural endogenous molecule, it lacks the serious side effects of common antiepileptic drugs used today. In the current study, we examined the potential antiepileptogenic effect of uridine in
Gregory L. Holmes, Qian Zhao, James R. Rusche, Tatiana Shatskikh, Ariane Marolewski   +4 more
openaire   +3 more sources

Uridine phosphorylase and uridine kinase activities of the Ehrlich ascites carcinoma

Canadian Journal of Biochemistry, 1969
Uridine phosphorylase and uridine kinase in cell-free extracts of the Ehrlich ascites carcinoma were assayed by a radioactive-tracer technique. Under the assay conditions uridine phosphorylase had an optimum pH of 8.0 and an apparent Km value for uridine of 2.5 × 10−3 M.
Rainnie, D J, Blair, D G
openaire   +3 more sources

Synthesis and Anti-HCV Activities of 4'-Fluoro-2'-Substituted Uridine Triphosphates and Nucleotide Prodrugs: Discovery of 4'-Fluoro-2'- C-methyluridine 5'-Phosphoramidate Prodrug (AL-335) for the Treatment of Hepatitis C Infection.

Journal of Medicinal Chemistry, 2019
We report the synthesis and biological evaluation of a series of 4'-fluoro-2'- C-substituted uridines. Triphosphates of the uridine analogues exhibited a potent inhibition of hepatitis C virus (HCV) NS5B polymerase with IC50 values as low as 27 nM. In an
Guangyi Wang, N. Dyatkina, M. Prhavc, C. Williams, V. Serebryany, Yujian Hu, Yongfei Huang, Jinqiao Wan, Xiangyang Wu, J. Deval, Amy D. Fung, Zhinan Jin, Hua Tan, K. Shaw, Hyunsoon Kang, Qingling Zhang, Yuen Tam, A. Stoycheva, A. Jekle, D. Smith, L. Beigelman   +20 more
semanticscholar   +1 more source

Uridyl Transferases and the Formation of Uridine Triphosphate: Enzymic Production of Uridine Triphosphate: Uridine Diphosphoglucose Pyrophosphorolysis

Nature, 1953
Uridyl Transferases and the Formation of Uridine Triphosphate: Enzymic Production of Uridine Triphosphate: Uridine Diphosphoglucose ...
Enrico Cutolo, Evelyn E. B. Smith, Herman M. Kalckar, Agnete Munch-Petersen   +3 more
openaire   +3 more sources

Uridine dynamic administration affects circadian variations in lipid metabolisms in the liver of high-fat-diet-fed mice

Chronobiology International, 2019
Carbohydrate and lipid metabolism in the liver has been reported to follow a certain circadian rhythm. Moreover, uridine supplementation also affected glucose and lipid homeostasis in previous studies; however, the mechanisms remain unclear.
Yi-lin Liu, Yumei Zhang, J. Yin, Z. Ruan, Xin Wu, Yulong Yin   +5 more
semanticscholar   +1 more source

Biochemistry of uridine in plasma

Clinica Chimica Acta, 2011
Uridine is a pyrimidine nucleoside that plays a crucial role in synthesis of RNA, glycogen, and biomembrane. In humans, uridine is present in plasma in considerably higher quantities than other purine and pyrimidine nucleosides, thus it may be utilized for endogenous pyrimidine synthesis.
Tetsuya Yamamoto, Yuji Moriwaki, Zenta Tsutsumi, Masafumi Kurajoh, Hidenori Koyama, Takuhito Shoji   +5 more
openaire   +3 more sources

ChemInform Abstract: BENZOYLAMINATION OF URIDINE DERIVATIVES

Chemischer Informationsdienst, 1976
AbstractAlternativ zu der sonst üblichen vielstufigen Synthese wird eine Methode zur Darstellung von Acylaminonucleosiden beschrieben.
Susumu Takizawa, Hiroo Morimato, Oyo Mitsunobu   +2 more
openaire   +4 more sources

Uridine Kinase: Altered Enzyme with Decreased Affinities for Uridine and CTP

Archives of Biochemistry and Biophysics, 1998
Uridine kinase is the rate-limiting enzyme in the salvage pathway for uridine or cytidine of mammalian cells. Alignment of the uridine kinase sequence with other nucleoside and nucleotide kinases supports a common ancestor for all of these. Three polypeptide segments for the ATP site and three polypeptide segments for the acceptor nucleoside site have ...
Thomas W. Traut, Patricia A. Ropp
openaire   +3 more sources