Results 91 to 100 of about 29,456 (288)

Determination of Enzyme Kinetic Parameters of UDP-glycosyltransferases

Bio-Protocol, 2013
The determination of enzyme kinetic parameters, such as the Km and kcat values, is an essential part of the characterization of newly discovered enzymes.
Jörg M. Augustin, Søren Bak
doaj   +1 more source

In vitro synthesis of particulate glycogen from uridine diphosphate glucose [PDF]

Archives of Biochemistry and Biophysics, 1969
Abstract High molecular weight glycogen has been prepared in vitro with liver glycogen synthetase (uridine diphosphate glucose: α-1,4-glucan α-4-glucosyltransferase, EC 2.4.1.11) and branching enzyme (α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase, EC 2.4.1.18) and uridine diphosphate glucose as glucose donor.
Parodi, Armando José A., Mordoh, Jose, Krisman, Clara R., Leloir, Luis Federico   +3 more
openaire   +4 more sources

Chronic Cellular NAD Depletion Activates a Viral Infection‐Like Interferon Response Through Mitochondrial DNA Leakage

Aging Cell, EarlyView.
Chronic NAD depletion in fibroblasts lowers NAD levels, increases mitochondrial mass, and triggers an interferon‐dependent inflammatory response via mtDNA leakage through VDAC1, activating the cGAS‐STING pathway. This links NAD deficiency to metabolic and inflammatory processes, with implications for diseases associated with NAD decline.
Claudia C. S. Chini, Laura Colman, Eduardo Palmieri, Jessica L. Strange, Sonu Kashyap, Bing Han, Andres Benitez‐Rosendo, Gina L. Ciccio Lopez, Sara Peixoto Rabelo, Shreyartha Mukherjee, Gustavo H. de Souza, John Varga, Ralph G. Meyer, Mirella L. Meyer‐Ficca, Eduardo N. Chini   +14 more
wiley   +1 more source

Metabolic profiling reveals coordinated switches in primary carbohydrate metabolism in grape berry (Vitis vinifera L.), a non-climacteric fleshy fruit [PDF]

, 2013
Changes in carbohydrate metabolism during grape berry development play a central role in shaping the final composition of the fruit. The present work aimed to identify metabolic switches during grape development and to provide insights into the timing of
Dai, Z., Delrot, S., Feil, R., Gomes, E., Leon, C., Lunn, J.   +5 more
core   +2 more sources

Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
Summary Transient expression in Nicotiana benthamiana offers a powerful and versatile platform for rapid production of complex specialized metabolites. Steroidal glycoalkaloids (SGAs) and steroidal saponins produced by members of the Solanaceae family are known for their diverse structures and activities including antimicrobial, anticancer, antiviral ...
Sachin A. Gharat, Adam Jozwiak, Ilana Rogachev, Elinor A. Sharon, Sagit Meir, Ashok P. Giri, Asaph Aharoni   +6 more
wiley   +1 more source

Regulation of hepatic glucose production and AMPK by AICAR but not by metformin depends on drug uptake through the equilibrative nucleoside transporter 1 (ENT1) [PDF]

, 2018
This is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.Aim: Recently we have observed differences in the ability of metformin and AICAR to repress glucose production from hepatocytes using 8CPT-cAMP.
Al-Jibouri, Argaud, Baldwin, Beebe, Boß, Cameron, Cameron, Cook, Corton, Foretz, Gadalla, Gonzalez-Benitez, Granner, Guigas, Gupte, Hasenour, Henion, Jarett, Jiang, Karsai, Lin, Lochhead, Meijer, Miller, Moseley, Ooba, Rena, Rena, Ritzel, Schmoll, Schmoll, Shu, Steculorum, Waidmann, Wang, Ward, Werner, Yao, Zhang, Zhou, Zhu, Zhu, Zimmerman   +42 more
core   +3 more sources

Unveiling the catalytical roles of three glycosyltransferases in triterpenoid saponins in quinoa

Plant Biotechnology Journal, EarlyView.
Summary Saponins constitute a class of characteristic compounds within quinoa grains. In this study, metabolomic analysis identified 20 triterpene saponins, the majority of which were exclusively localized within the pericarp. Furthermore, three crucial glycosyltransferases were identified in quinoa.
Yiming Zhang, Peng Zhu, Long Wu, Zhaohui Li, Yongshun Huang, Mingyu Wang, Kaiying Gu, Peng Wang, Jun Li, Huihua Wan, Hailong Zhang, Jianghua Chen, Ruikun He, Wei Sun, Lixin Li   +14 more
wiley   +1 more source

Bilirubin - Uridine Diphosphate Glucuronosyltransferase (Ugt1a1) Gene Mutations Among Newborn Babies In The Malay Population In Kelantan With Hyperbilirubinaemia [PDF]

, 2005
Sind rom Gilbert berpunca daripada keabnormalan pada gen uridine diphosphate-glucuronosyltransferase 1A 1 (UGT1A 1) yang disebabkan oleh mutasi. Mutasi-mutasi ini berbeza bagi setiap populasi dan kebanyakannya menjadi faktor asas bagi jaundis di ...
Yusoff, Surini
core  

In Vitro Model for Hepatotoxicity Studies Based on Primary Human Hepatocyte Cultivation in a Perfused 3D Bioreactor System [PDF]

, 2016
Accurate prediction of the potential hepatotoxic nature of new pharmaceuticals remains highly challenging. Therefore, novel in vitro models with improved external validity are needed to investigate hepatic metabolism and timely identify any toxicity ...
Damm, Georg, De Bondt, An, Freyer, Nora, Jacobs, Frank, Knöspel, Fanny, Monshouwer, Mario, Richter, Marco, Seehofer, Daniel, Snoeys, Jan, Strahl, Nadja, Wyngaert, Ilse van den, Zeilinger, Katrin   +11 more
core   +2 more sources

Metabolic Profile Associated With Encystation in Acanthamoeba

Journal of Eukaryotic Microbiology, Volume 72, Issue 5, September/October 2025.
ABSTRACT The genus Acanthamoeba includes widespread protozoa that can cause severe infections in humans. Their ability to form resistant cysts within infected tissues complicates treatment, making it essential to understand the encystation process for developing effective therapeutic strategies.
Cecília Cirelli, Isabela Aurora Rodrigues, Jéssica Gardone Vitório, Filipe Fideles Duarte‐Andrade, Gisele André Baptista Canuto, Leiliane Coelho André, Juliano Simões de Toledo, Ana Paula Fernandes, Adriana Oliveira Costa   +8 more
wiley   +1 more source