Results 111 to 120 of about 73,565 (199)

Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity

New Phytologist, EarlyView.
Polyphenol oxidases (PPOs) depletion preserves endogenous proteins at their predicted molecular weights and enhances detectable enzymatic activities. (a) Less RbcL crosslinking in ppo mutant of Nicotiana benthamiana. Leaf extracts were incubated for 1 h, separated on protein gels, stained with Coomassie or analyzed by Western blot with the anti‐RbcL ...
Kaijie Zheng, Farnusch Kaschani, Emma C. Watts, Markus Kaiser, Renier A. L. van der Hoorn   +4 more
wiley   +1 more source

<i>Avibacterium paragallinarum</i>: Pathogenesis Mechanisms and Subunit Vaccine Development. [PDF]

Microorganisms
Li Z, Liu Y, Liu Z, Jiang Z, Wang Y, Xing B, Mei C, Wang H.   +7 more
europepmc   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

<i>Poria cocos</i> Polysaccharide Fraction PCP-II Enhances Humoral and Cellular Responses to a SARS-CoV-2 RBD Subunit Vaccine in Mice. [PDF]

Vaccines (Basel)
Zhou M, Liu J, Zhang X, Yan F, Wu Y, Huang C, Xie D, Liu B.   +7 more
europepmc   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Porcine Vaccines Contribute to Enhancing Health, Productivity, and Animal Welfare. [PDF]

Vaccines (Basel)
An Z, Di S, An T.
europepmc   +1 more source

Plant‐Based Production of Human Major Histocompatibility Complex Class II Molecules

Plant Biotechnology Journal, EarlyView.
ABSTRACT Major histocompatibility complex class II (MHC II) protein triggers adaptive immune responses by presenting antigens on antigen‐presenting cells (APCs) to be recognised by CD4+ T cells. Recombinant MHC II proteins are effective for the analysis of peptide‐binding interaction, detection of antigen‐specific T‐cells and clinical testing for donor‐
Abdelaziz Ramadan, Hiroko Miyadera, Kaho Oka, Kohei Ishikawa, Toshiya Kobayashi, Misaki Kobayashi, Mitsunori Shiroishi, Emiko Noguchi, Kenji Miura   +8 more
wiley   +1 more source

Trimeric hemagglutinin vaccine provides chickens complete protection against lethal H5 subtype avian influenza virus from clade 2.3.4.4b. [PDF]

Emerg Microbes Infect
Chen T, Huang Q, Chen X, Zhu J, Kong D, Liao X, Liao M, Fan H.   +7 more
europepmc   +1 more source

Antibiotic‐mediated immune modulation in periodontitis

Periodontology 2000, EarlyView.
Abstract Periodontitis is a chronic inflammatory disease affecting the supporting structures of the teeth. Although initiated by dysbiotic microbial communities, its progression is largely driven by the host's uncontrolled inflammatory response. While antibiotics have conventionally been employed in periodontitis therapy for their antimicrobial ...
Lina J. Suárez, R. M. Arce, Camila Pinheiro Furquim, Cristiane Gonçalves, Alpdogan Kantarci, Magda Feres, Nidia C. Castro dos Santos   +6 more
wiley   +1 more source

Advances in African swine fever vaccine development: challenges and prospects. [PDF]

J Vet Sci
Utomo DIS, Dharmayanti NI, Putra MY.
europepmc   +1 more source