Whole lifespan microscopic observation of budding yeast aging through a microfluidic dissection platform [PDF]
, 2012 Important insights into aging have been generated with the genetically tractable and short-lived budding yeast. However, it is still impossible today to continuously track cells by high-resolution microscopic imaging (e.g., fluorescent imaging ...
Barral +25 more
core +12 more sources
ATP-independent Control of Vac8 Palmitoylation by a SNARE Subcomplex on Yeast Vacuoles [PDF]
, 2005 Yeast vacuole fusion requires palmitoylated Vac8. We previously showed that Vac8 acylation occurs early in the fusion reaction, is blocked by antibodies against Sec18 (yeast N-ethylmaleimide-sensitive fusion protein (NSF)), and is mediated by the R-SNARE
Cristodero, Marina +5 more
core +1 more source
In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis. [PDF]
, 2016 UnlabelledToxoplasma gondii is an obligate intracellular parasite that invades host cells and replicates within a unique parasitophorous vacuole. To maintain this intracellular niche, the parasite secretes an array of dense granule proteins (GRAs) into ...
Bell, Hannah N +7 more
core +2 more sources
Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts. [PDF]
, 2015 Organelle contact sites perform fundamental functions in cells, including lipid and ion homeostasis, membrane dynamics, and signaling. Using a forward proteomics approach in yeast, we identified new ER-mitochondria and ER-vacuole contacts specified by an
Murley, Andrew +5 more
core +2 more sources
Autophagy extends lifespan via vacuolar acidification
Microbial Cell, 2014 Methionine restriction (MetR) is one of the rare regimes that prolongs lifespan across species barriers. Using a yeast model, we recently demonstrated that this lifespan extension is promoted by autophagy, which in turn requires vacuolar acidification ...
Christoph Ruckenstuhl +21 more
doaj +1 more source
Nature Reviews Microbiology, 2018 Two recent studies provide new insights into the architecture, molecular mechanisms and function of the Plasmodium translocon of exported proteins (PTEX) complex and the core PTEX protein EXP2.
openaire +2 more sources
Calcium Signals from the Vacuole
Plants, 2013 The vacuole is by far the largest intracellular Ca2+ store in most plant cells. Here, the current knowledge about the molecular mechanisms of vacuolar Ca2+ release and Ca2+ uptake is summarized, and how different vacuolar Ca2+ channels and Ca2+ pumps may
Gerald Schönknecht
doaj +1 more source
The water to solute permeability ratio governs the osmotic volume dynamics in beetroot vacuoles
Frontiers in Plant Science, 2016 Plant cell vacuoles occupy up to 90% of the cell volume and, beyond their physiological function, are constantly subjected to water and solute exchange.
Victoria Vitali +5 more
doaj +1 more source
The c-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi-mediated sorting to the vacuole [PDF]
, 2003 We have assessed the ability of the plant secretory pathway to handle the expression of complex heterologous proteins by investigating the fate of a hybrid immunoglobulin A/G in tobacco cells.
Denecke, J. (Jurgen) +6 more
core +2 more sources
SUT sucrose and MST monosaccharide transporter inventory of the Selaginella genome
Frontiers in Plant Science, 2012 While most metazoa mainly use hexose transporters to acquire hexoses from their diet and as a transport form for distributing carbon and energy within their bodies, insects use trehalose and plants use sucrose as their major form for translocation. Plant
Sylvie eLalonde, Wolf B Frommer
doaj +1 more source