Results 21 to 30 of about 441,117 (305)
A microscopy-based kinetic analysis of yeast vacuolar protein sorting
eLife, 2020 Saccharomyces cerevisiae is amenable to studying membrane traffic by live-cell fluorescence microscopy. We used this system to explore two aspects of cargo protein traffic through prevacuolar endosome (PVE) compartments to the vacuole.
Jason C Casler, Benjamin S Glick
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Vps13-Mcp1 interact at vacuole–mitochondria interfaces and bypass ER–mitochondria contact sites
Journal of Cell Biology, 2017 Membrane contact sites between endoplasmic reticulum (ER) and mitochondria, mediated by the ER–mitochondria encounter structure (ERMES) complex, are critical for mitochondrial homeostasis and cell growth.
Arun T. John Peter +5 more
semanticscholar +1 more source
Frontiers in Plant Science, 2023 The aspartic proteinase cardosin A is a vacuolar enzyme found to accumulate in protein storage and lytic vacuoles in the flowers and protein bodies in the seeds of the native plant cardoon.
Cláudia Pereira +3 more
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TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy
bioRxiv, 2019 Cellular adaptation in response to nutrient limitation requires the induction of autophagy and lysosome biogenesis for the efficient recycling of macromolecules. Here, we discovered that starvation and TORC1 inactivation not only lead to the upregulation
Xi Yang +10 more
semanticscholar +1 more source
Quantitation of vacuolar sugar transporter abundance changes using QconCAT synthtetic peptides
Frontiers in Plant Science, 2016 Measurements of protein abundance changes are important for biological conclusions on protein-related processes such as activity or complex formation. Proteomic analyses in general are almost routine tasks in many laboratories.
Heidi ePertl-Obermeyer +4 more
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Touch and Go: Membrane Contact Sites Between Lipid Droplets and Other Organelles
Frontiers in Cell and Developmental Biology, 2022 Lipid droplets (LDs) have emerged not just as storage sites for lipids but as central regulators of metabolism and organelle quality control. These critical functions are achieved, in part, at membrane contact sites (MCS) between LDs and other organelles.
Pin-Chao Liao +9 more
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Reconstitution reveals Ykt6 as the autophagosomal SNARE in autophagosome–vacuole fusion
Journal of Cell Biology, 2018 Autophagy mediates the bulk degradation of cytoplasmic material, particularly during starvation. Upon the induction of autophagy, autophagosomes form a sealed membrane around cargo, fuse with a lytic compartment, and release the cargo for degradation ...
L. Bas +6 more
semanticscholar +1 more source
Cell Reports, 2021 Summary: To maintain secretory pathway fidelity, misfolded proteins are commonly retained in the endoplasmic reticulum (ER) and selected for ER-associated degradation (ERAD).
Zhihao Sun +2 more
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Membrane protein recycling from the vacuole/lysosome membrane
Journal of Cell Biology, 2018 The lysosome (or vacuole in yeast) is the central organelle responsible for cellular degradation and nutrient storage. Lysosomes receive cargo from the secretory, endocytic, and autophagy pathways.
Sho W. Suzuki, S. Emr
semanticscholar +1 more source
Legionella RavZ Plays a Role in Preventing Ubiquitin Recruitment to Bacteria-Containing Vacuoles
Frontiers in Cellular and Infection Microbiology, 2017 Bacterial pathogens like Salmonella and Legionella establish intracellular niches in host cells known as bacteria-containing vacuoles. In these vacuoles, bacteria can survive and replicate.
Tomoko Kubori +5 more
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