Real-time PCR for the rapid detection of vanA, vanB and vanM genes
Background/Purpose: To evaluate the ability of quadruple Taqman probe real-time PCR to the detection of vanA, vanB and vanM in enterococcal isolates. Methods: A total of 343 strains, including 253 vancomycin-resistant enterococcus (VRE) strains and 90 ...
Yuan-Hui He +6 more
doaj +3 more sources
Detection of VRE: vanA and vanB Genes by PCR
In clinical laboratories the phenotypic detection of low-level vancomycin resistance in enterococci is problematic. Rapid detection of vancomycin resistant enterococci (VRE), in particular those that carry the vanA or vanB genes, may minimize transmission within a hospital and therefore reduce the risk of colonization and infection.
Merlino, J. +3 more
core +4 more sources
Clostridium clostridioforme and Atopobium minutum Clinical Isolates with VanB-Type Resistance in France [PDF]
ABSTRACT Acquired vancomycin resistance in Gram-positive anaerobes has been reported only in Australia and Canada from rare vanB -positive stool samples in the absence of vancomycin-resistant enterococci (VRE). We report the emergence of VanB-type resistance in Clostridium clostridioforme
Jean-Christophe, Marvaud +2 more
openaire +3 more sources
Surveillance of Vancomycin-resistant Enterococci colonization with GeneXpert vanA/vanB test and culture method [PDF]
Objectives: Early detection of vancomycin resistant enterococci (VRE) colonization in hospitalized patients plays an important role in controlling enterococcal infections.
Hatice Uludağ Altun +6 more
doaj +3 more sources
Outbreak of multidrug-resistant Enterococcus faecium with transferable vanB class vancomycin resistance [PDF]
Enterococcus faecium strains resistant to ampicillin, high levels of gentamicin, and vancomycin but susceptible to teicoplanin (vanB class vancomycin resistance) were recovered from 37 patients during an outbreak involving a 250-bed university-affiliated hospital.
J M, Boyce +8 more
openaire +3 more sources
Molecular Diagnostics for WHO Priority Bacterial Pathogens: A Bibliometric Mapping of Diagnostic Platforms, Resistance Markers, and Antimicrobial Resistance Research Trends. [PDF]
This bibliometric mapping of 1746 publications (2000–2025) charts molecular diagnostics for WHO priority bacterial pathogens across platforms, targets, and resistance markers. Conventional PCR/NAAT and whole‐genome sequencing emerge as complementary core platforms, and the field evolves from targeted pathogen detection into an AMR‐centered ...
Ünlü S.
europepmc +2 more sources
The Structural Basis for Induction of VanB Resistance [PDF]
Because teicoplanin and vancomycin are the last line of defense for many bacterial infections, the emergence of resistance to glycopeptide antibiotics in enterococci and streptococci has aroused concern. Despite their similarity in terms of structure and mechanism of action, vancomycin induces the expression of genes that leads to bacterial resistance,
Steven D, Dong +7 more
openaire +3 more sources
Plasmid mediated VanB Glycopeptide resistance in Enterococci.
Enterococcus faecium, which was highly resistant to vancomycin (MIC 256 mg/liter), but susceptible to teicoplanin (MIC 2 mg/liter), caused two distinct episodes of infection on a renal unit in the United Kingdom.
Hasting, J G +6 more
core +5 more sources
VanB-VanC1 Enterococcus gallinarum, Italy
To the Editor: We report detecting a vanB determinant in Enterococcus gallinarum in poultry in Italy. High-level vanA-mediated glycopeptide resistance has been described for E. gallinarum and E. casseliflavus (1–4), and vanB-mediated vancomycin resistance has been frequently described for E. faecalis and E. faecium. However, vanB-mediated resistance in
Caterina Mammina +3 more
doaj +5 more sources
Characterization of Vancomycin Resistant Enterococci in Hospitalized Patients and Role of Gut Colonization [PDF]
Introduction: Enterococci are part of the normal intestinal flora and have been recognized as important human pathogens. Vancomycin Resistant Enterococci (VRE) are global threat as this resistance is transmissible and also poses a challenge for ...
Geetarani Purohit +6 more
doaj +1 more source

