Results 51 to 60 of about 236,010 (265)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Depletion of Mitochondrial Cyclophilin D in Endothelial and Smooth Muscle Cells Attenuates Vascular Dysfunction and Hypertension

Function
Hypertension is a major risk factor of cardiovascular disease affecting nearly half of adult population. It is associated with mitochondrial dysfunction and understanding these mechanisms is important to develop new therapies.
Anna Dikalova, Mingfang Ao, Louise Lantier, Sergey Gutor, Sergey Dikalov   +4 more
doaj   +1 more source

Activation and Contraction of Human “Vascular” Smooth Muscle Cells Grown From Circulating Blood Progenitors

Frontiers in Cell and Developmental Biology, 2021
Blood outgrowth smooth muscle cells (BO-SMCs) offer the means to study vascular cells without the requirement for surgery providing opportunities for drug discovery, tissue engineering, and personalized medicine.
Blerina Ahmetaj-Shala, Isra Marei, Isra Marei, Ryota Kawai, Ryota Kawai, Stephen Rothery, Charis Pericleous, Nura A. Mohamed, Nura A. Mohamed, Hime Gashaw, Kalliopi Bokea, Jake Samuel, Annabelle Vandenheste, Fisnik Shala, Nicholas S. Kirkby, Jane A. Mitchell   +15 more
doaj   +1 more source

Vascular Smooth Muscle Cells in the Pathogenesis of Vascular Calcification [PDF]

Circulation Research, 2009
See related article, pages 733–741 Vascular calcification is a major risk factor for cardiovascular morbidity and mortality. In atherosclerotic lesions, calcification is mainly found in the neointima of atheromatous plaques and has been shown to positively correlate with the plaque burden and the risk of myocardial infarction.
openaire   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

MicroRNA26 attenuates vascular smooth muscle maturation via endothelial BMP signalling.

PLoS Genetics, 2019
As small regulatory transcripts, microRNAs (miRs) act as genetic 'fine tuners' of posttranscriptional events, and as genetic switches to promote phenotypic switching. The miR miR26a targets the BMP signalling effector, smad1.
Charlene Watterston, Lei Zeng, Abidemi Onabadejo, Sarah J Childs   +3 more
doaj   +1 more source

Guidance Molecules in Vascular Smooth Muscle

Frontiers in Physiology, 2018
Several highly conserved families of guidance molecules, including ephrins, Semaphorins, Netrins, and Slits, play conserved and distinct roles in tissue remodeling during tissue patterning and disease pathogenesis.
Alexandra Christine Finney, Anthony Wayne Orr, Anthony Wayne Orr, Anthony Wayne Orr   +3 more
doaj   +1 more source

Acute oxygen sensing by vascular smooth muscle cells

Frontiers in Physiology, 2023
An adequate supply of oxygen (O2) is essential for most life forms on earth, making the delivery of appropriate levels of O2 to tissues a fundamental physiological challenge.
Alejandro Moreno-Domínguez, Alejandro Moreno-Domínguez, Alejandro Moreno-Domínguez, Olaia Colinas, Olaia Colinas, Olaia Colinas, Tarik Smani, Tarik Smani, Juan Ureña, Juan Ureña, José López-Barneo, José López-Barneo, José López-Barneo   +12 more
doaj   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source