Results 91 to 100 of about 11,641 (203)

Protocol for the production and reconstitution of VDAC1 for functional assays

STAR Protocols
The voltage-dependent anion channel (VDAC) is an abundant and multifunctional outer mitochondrial membrane protein, playing key roles in neurodegeneration, apoptosis, and mitochondrial membrane biogenesis. Here, we present a protocol to produce and reconstitute high yields of detergent-solubilized VDAC, expressed as inclusion bodies in E. coli.
Dearden, Grace, Ravishankar, Varun, Sakata, Ken-Taro, Menon, Anant, Bergdoll, Lucie   +4 more
openaire   +3 more sources

R‐Loops Mediated Odontogenic Differentiation of hDPSCs by Activating the cGAS‐STING Pathway Under Inflammatory Microenvironment

Oral Diseases, EarlyView.
ABSTRACT Background R‐Loops are three‐stranded DNA/RNA hybrids implicated in immune responses. Their role in mesenchymal stem cell differentiation, particularly in dental pulp regeneration under inflammation, remains unclear. Methods Rat pulpo‐dentinal complex (PDC) injury models and LPS‐stimulated human dental pulp stem cells (hDPSCs) were used.
Yun Yang, Yiqing Wang, Yating Miao, Zhipu Luo, Lin Niu, Ruirui Liu   +5 more
wiley   +1 more source

Mitochondrial Ca2+ uniporter haploinsufficiency leads to sexually dimorphic redox imbalance and metabolic remodelling in the mouse brain

The Journal of Physiology, EarlyView.
Abstract figure legend The genetic inactivation of one Mcu allele leads to sex‐specific changes in neuronal function in adult mice, that is, the firing of action potentials and the relationship between cytosolic and mitochondrial Ca2+ levels. The ability to produce NAD(P)H by stimulated neural tissue is largely preserved in male mice but delayed in ...
Jenna Gray, Girish Halemirle, Beatriz Ferrán, Hadyn Rose, Traci L. Redwine, Sophia Pham, Bo Hagy, Atul Pranay, Jennifer Giorgione, Stacy A. Hussong, Veronica Galvan, Kenneth Humphries, Holly Van Remmen, Mike Kinter, William E. Sonntag, Pankaj K. Singh, Carlos Manlio Díaz‐García   +16 more
wiley   +1 more source

Proteomic analysis of cellular models of neurodegeneration and mitochondrial dysfunction [PDF]

, 2009
Mitochondrial dysfunction is thought to contribute to neurodegenerative processes. As an example, dysfunction of complex I of the electron transport chain has been observed in Parkinson’s disease patients and 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (
Burté, F, Burte, Florence
core  

Demonstration of beat‐to‐beat, on‐demand ATP synthesis in ventricular myocytes reveals sex‐specific mitochondrial and cytosolic dynamics

The Journal of Physiology, EarlyView.
Abstract figure legend Beat‐locked mitochondrial ATP transients reveal modular, sex‐specific bioenergetic control during excitation–contraction coupling. A, each action potential activates L‐type CaV1.2 channels, producing a Ca2+ influx that triggers ryanodine receptors (RyR2) and elicits SR Ca2+ release.
Paula Rhana, Collin Matsumoto, L. Fernando Santana   +2 more
wiley   +1 more source

Microenvironment‐Responsive Nanomedicine Enables Vertical Modulation of Mitochondrial Pathological Networks in Myocardial Ischemia–Reperfusion Injury

Advanced Science, Volume 13, Issue 55, 5 October 2026.
A mitochondria‐targeted, microenvironment‐responsive nanoplatform enables pH/ROS‐triggered co‐delivery of berberine and isoliensinine to orchestrate ROS scavenging, inflammation suppression, and apoptosis inhibition. This vertical modulation of mitochondrial pathological networks preserves mitochondrial function and effectively mitigates myocardial ...
Jue Wang, Jia Zhou, Wenqin Yuan, Tianjiao Zhao, Yunying Huang, Yingci Xia, Yuting Lin, Fei Li, Qiong Huang, Chong Liu, Kelong Ai, Qun Qin   +11 more
wiley   +1 more source

PANoptosis In Microglia and Neurons Following Traumatic CNS Injury: A Systematic Review of Pathological Features and Therapeutic Implications

CNS Neuroscience &Therapeutics, Volume 32, Issue 10, October 2026.
Following traumatic CNS injury, PANoptosis is activated in microglia and neurons, with microglia–neuron crosstalk serving as a key feature of this process. The PANoptosome sensors ZBP1 and NLRP12, together with the adaptor ASC, activate downstream executioner proteins to drive PANoptosis.
Zhusheng Feng, Yingnan Fan, Tianshu Du, Peipei Huang, Yuqin Ye, Junjie Li   +5 more
wiley   +1 more source

Tissue‐Specific Isolation of Hepatocyte Extracellular Vesicles From Human Plasma Enables Tissue‐Resolved Proteomic and miRNA Profiling

Journal of Extracellular Vesicles, Volume 15, Issue 10, October 2026.
ABSTRACT Tissue‐specific resolution remains a critical barrier in liquid biopsy for precision medicine. To address this, we developed NEXPLOR (Novel EXtracellular vesicle PopuLation Omics Revealer), an immunoaffinity platform enabling selective enrichment of circulating tissue‐specific extracellular vesicles (TS‐EVs), demonstrated here for liver ...
Ricardo Figueiras, Susana Vagueiro, Raissa Kay, Elnaz Persia, Roger de Alwis, Razan Hijazi, Brian Davidson, Kristine von Bargen, Pierre Arsène, Tomás Dias   +9 more
wiley   +1 more source

HSP90AA1–VDAC1 interaction contributes to breast cancer progression and doxorubicin resistance through PI3K/AKT signaling

Breast Cancer Research
Background Chemotherapy resistance remains a major obstacle to durable control of breast cancer (BC), particularly to doxorubicin (DOX). Heat shock protein 90 alpha family class A member 1 (HSP90AA1) and the mitochondrial outer-membrane channel VDAC1 ...
Yutong Fang, Cuiping Guo, Fengtao Zheng, Yefeng Xiao, Junpeng Liu, Yongqu Zhang, Jundong Wu   +6 more
doaj   +1 more source

Abnormal alpha-synuclein reduces nigral voltage-dependent anion channel 1 in sporadic and experimental Parkinson's disease

Neurobiology of Disease, 2014
Both the misfolding of α-synuclein and mitochondrial dysfunction are considered two major contributors to Parkinson's disease (PD). However, the relationship between the two in normal and PD states remains unclear.
Yaping Chu, Jennifer G. Goldman, Leo Kelly, Yinzhen He, Tracy Waliczek, Jeffrey H. Kordower   +5 more
doaj   +1 more source