Results 191 to 200 of about 41,395 (219)

Engineering a Low-Immunogenic Mirror-Image VHH against Vascular Endothelial Growth Factor.

ACS Chemical Biology
Immunogenicity is a major caveat of protein therapeutics. In particular, the long-term administration of protein therapeutic agents leads to the generation of antidrug antibodies (ADAs), which reduce drug efficacy while eliciting adverse events.
Keisuke Aoki, Katsuaki Higashi, Sakiho Oda, Asako Manabe, Kayuu Maeda, Jyoji Morise, S. Oka, S. Inuki, Hiroaki Ohno, Shinya Oishi, Motohiro Nonaka   +10 more
semanticscholar   +1 more source

Production of Designer VHH-Based Antibodies in Plants

2022
Simplified monoclonal antibodies can be produced by fusing a VHH or nanobody, derived from camelid heavy-chain-only antibodies to the Fc domain of either IgG (VHH-IgG), IgA (VHH-IgA), or IgY (VHH-IgY). These recombinant antibodies are encoded by a single gene and their production can be easily scaled up in plants.
openaire   +2 more sources

Small-Scale Secretory VHH Expression in Saccharomyces cerevisiae

2022
After isolation of a single-domain antibody (VHH) binding to an antigen of interest, the soluble VHH is often produced in Escherichia coli. However, targeting VHH expression to the secretory pathway of Saccharomyces cerevisiae (baker's yeast) enables the secretion of correctly folded, soluble, disulfide-bonded, and N-glycosylated VHHs into the culture ...
Harmsen, Michiel M., van Hagen-van Setten, Marga, Willemsen, Peter T.J.   +2 more
openaire   +2 more sources

Anti lag3 vhhs and their use

2022
The present disclosure relates to field of immune checkpoints in onco-immunology and their identification as potential targets for the treatment of cancer patients : it provides VHHs (Single-domain antibody fragments, also known as 'heavy-chain variable domains' or 'nanobodies') to detect and quantify in a non-invasive way the immune checkpoint LAG-3.
Breckpot, Karine, Broos, Katrijn, Devoogdt, Nick, Lecocq, Quentin, Keyaerts, Marleen, Raes, Geert   +5 more
openaire   +4 more sources

Structure and Function of Camelid VHH

2016
The humoral immune response of camels and llama’s is unique in containing two types of IgG antibodies: classical heterotetrameric and homodimeric heavy chain-only antibodies (HCAbs). Remarkably, these heavy chain antibodies are functional in antigen binding and reach high titers and affinities after immunizing a camelid. Antigen recognition by HCAbs is
Vincke, Cecile, Muyldermans, Serge
openaire   +2 more sources

INJECTABLE HYDROGELS FOR VHH DELIVERY TO RESTORE JOINT HOMEOSTASIS

Orthopaedic Proceedings
Osteoarthritis (OA) is a chronic joint disease characterized by cartilage degradation and inflammation driven by pro-inflammatory cytokines like Tumor Necrosis Factor-alpha (TNF-α). Neutralizing TNF-α using antibody fragments is a promising therapeutic approach, yet rapid clearance limits the effectiveness of intra-articular treatments.
Serrano Larrea, Carolina, Morshuis, Lisanne, Zhong, Lin, Zambonino, Marjorie, Le, Thanh, Semmekrot, Fleur, Zoetebier, Bram, Karperien, Marcel   +7 more
openaire   +1 more source

Introduction of PMMA-Tag to VHH for improving recovery and immobilization rate of VHHs

3rd Annual International Conference on Chemistry, Chemical Engineering and Chemical Process (CCECP 2015), 2015
Bongmun Kang, Yoiichi Kumada
openaire   +1 more source

Development of an ^18F-labeled anti-human CD8 VHH for same-day immunoPET imaging

European Journal of Nuclear Medicine and Molecular Imaging, 2022
S. Sriraman, C. Davies, H. Gill, J. Kiefer, Jianping Yin, A. Ogasawara, A. Urrutia, Vincent Javinal, Zhonghua Lin, D. Seshasayee, Ryan Abraham, P. Haas, C. Koth, J. Mařík, J. T. Koerber, Simon-Peter Williams   +15 more
semanticscholar   +1 more source

An alpaca single-domain antibody (VHH) phage-display library constructed by CDR shuffling provided high affinity VHHs against desired protein antigens.

International Immunology, 2022
Narutoshi Tsukahara, A. Murakami, Maiko Motohashi, H. Nakayama, Yoshirō Kondō, Yuji Ito, T. Azuma, H. Kishimoto   +7 more
semanticscholar   +1 more source

VHH

2006
openaire   +1 more source