Results 61 to 70 of about 928,374 (298)

Deciphering the Human Virome with Single-Virus Genomics and Metagenomics

Viruses, 2018
Single-cell genomics has unveiled the metabolic potential of dominant microbes inhabiting different environments, including the human body. The lack of genomic information for predominant microbes of the human body, such as bacteriophages, hinders our ...
Maria José de la Cruz Peña, Francisco Martinez-Hernandez, Inmaculada Garcia-Heredia, Mónica Lluesma Gomez, Òscar Fornas, Manuel Martinez-Garcia   +5 more
doaj   +1 more source

Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing. [PDF]

PLoS ONE, 2017
Viral sewage metagenomics is a novel field of study used for surveillance, epidemiological studies, and evaluation of waste water treatment efficiency. In raw sewage human waste is mixed with household, industrial and drainage water, and virus particles ...
Mathis Hjort Hjelmsø, Maria Hellmér, Xavier Fernandez-Cassi, Natàlia Timoneda, Oksana Lukjancenko, Michael Seidel, Dennis Elsässer, Frank M Aarestrup, Charlotta Löfström, Sílvia Bofill-Mas, Josep F Abril, Rosina Girones, Anna Charlotte Schultz   +12 more
doaj   +1 more source

Comparing Viral Metagenomic Extraction Methods [PDF]

Current Issues in Molecular Biology, 2017
A crucial step in the molecular detection of viruses in clinical specimens is the efficient extraction of viral nucleic acids. The total yield of viral nucleic acid from a clinical specimen is dependent on the specimen's volume, the initial virus concentration and the effectiveness provided by the extraction method.
Jeanette, Klenner, Claudia, Kohl, Piotr Wojtek, Dabrowski, Andreas, Nitsche   +3 more
openaire   +2 more sources

New Viruses in Idiopathic Human Diarrhea Cases, the Netherlands

Emerging Infectious Diseases, 2014
Emerging viral infections can be identified by using a viral metagenomics approach for clinical human material. Diarrhea samples of patients with unexplained gastroenteritis from the Netherlands were analyzed by using viral metagenomics.
Saskia L. Smits, Claudia M.E. Schapendonk, Janko van Beek, Harry Vennema, Anita C. Schürch, Debby Schipper, Rogier Bodewes, Bart L. Haagmans, Albert D. M. E. Osterhaus, Marion P. Koopmans   +9 more
doaj   +1 more source

Assembly of viral genomes from metagenomes [PDF]

Frontiers in Microbiology, 2014
Viral infections remain a serious global health issue. Metagenomic approaches are increasingly used in the detection of novel viral pathogens but also to generate complete genomes of uncultivated viruses. In silico identification of complete viral genomes from sequence data would allow rapid phylogenetic characterization of these new viruses.
Saskia L Smits, Saskia L Smits, Rogier eBodewes, Aritz eRuiz-Gonzalez, Aritz eRuiz-Gonzalez, Aritz eRuiz-Gonzalez, Wolfgang eBaumgärtner, Marion P Koopmans, Marion P Koopmans, Albert D. M. E Osterhaus, Albert D. M. E Osterhaus, Anita C Schürch   +11 more
openaire   +5 more sources

Structural characterization of a soil viral auxiliary metabolic gene product – a functional chitosanase

Nature Communications, 2022
Metagenomics is revealing auxiliary metabolic genes (AMGs) in soil viral genomes. Here, authors solve the crystal structure for a soil viral AMG product, free and ligand bound, and show the protein can decompose chitin, a common carbon polymer.
Ruonan Wu, Clyde A. Smith, Garry W. Buchko, Ian K. Blaby, David Paez-Espino, Nikos C. Kyrpides, Yasuo Yoshikuni, Jason E. McDermott, Kirsten S. Hofmockel, John R. Cort, Janet K. Jansson   +10 more
doaj   +1 more source

Viral Metagenomics Revealed Sendai Virus and Coronavirus Infection of Malayan Pangolins (Manis javanica)

Viruses, 2019
Pangolins are endangered animals in urgent need of protection. Identifying and cataloguing the viruses carried by pangolins is a logical approach to evaluate the range of potential pathogens and help with conservation.
Ping Liu, Wu Chen, Jinping Chen
semanticscholar   +1 more source

Bioinformatics for Viral Metagenomics [PDF]

Journal of Data Mining in Genomics & Proteomics, 2013
Detection of the presence of known and unknown viruses in biospecimens is today routinely performed using viral metagenomics. Because the sequencing speed and cost per base is rapidly declining with new next generation sequencing technologies, such as HiSeq (Illumina), 454 GS FLX (Roche), SOLiD (ABI) and Ion Torrent Proton (Life Technologies), the ...
openaire   +1 more source

Applications of viral metagenomics in the veterinary field [PDF]

, 2010
Viral metagenomics provide a powerful technology to investigate the viral flora of healthy and sick animals. Using these methodologies, we gain a better understanding in the etiology of diseases, as well as deepen our knowledge into the viruses ...
Blomström, Anne-Lie
core  

A library preparation optimized for metagenomics of RNA viruses

, 2021
Our understanding of the viral communities associated to animals has not yet reached the level attained on the bacteriome. This situation is due to, among others, technical challenges in adapting metagenomics using high‐throughput sequencing to the study
Etienne Loire, Albane Marie, Fall, Assane Gueye, Julie Reveillaud, Emmanuel Albina, Reveillaud, Julie, Virginie Dupuy, Gimonneau, Geoffrey, Biram Biteye, Koual, Rachid, Biteye, Biram, Lambert, Grégory, Claire Garros, Exbrayat, Antoni, Garros, Claire, Marc Eloit, Loire, Etienne, Assane G. Fall, Balenghien, Thomas, Fall, Assane, Dupuy, Virginie, Patricia Gil, Geoffrey Gimonneau, Momar Talla Seck, Serafin Gutierrez, Thomas Balenghien, Gutiérrez, Serafin, Antoni Exbrayat, Albina, Emmanuel, Ignace Rakotoarivony, Talla Seck, Momar, Talla Seck, Monar, Francés, Benoit, Gregory Lambert, Rakotoarivony, Ignace, Rachid Koual, Benoît Frances, Marie, Albane, Eloit, Marc, Gil, Patricia   +39 more
core   +1 more source