Results 121 to 130 of about 156,575 (279)

Hydroxyapatite Chromatography of Phage-Display Virions

BioTechniques, 2005
Hydroxyapatite column chromatography can be used to purify filamentous bacteriophage--the phage most commonly used for phage display. Virions that have been partially purified from culture supernatant by two cycles of precipitation in 2% polyethylene glycol are adsorbed onto the matrix at a density of at least 7.6 x 10(13) virions (about 3 mg) per ...
George P. Smith, Todd R. Gingrich
openaire   +3 more sources

Immune non‐response despite effective antiretroviral therapy in people living with HIV: A review of potential mechanisms, biomarkers and therapeutic approaches

HIV Medicine, EarlyView.
Abstract Introduction A proportion of people living with HIV (PLWH) fail to restore their CD4 count or their CD4/CD8 ratio despite effective antiretroviral therapy (ART). PLWH with immune non‐response (INR) are at a higher risk of both AIDS and non‐AIDS events. The underlying mechanisms of INR remain unclear.
Charlotte Silvestre, Antoine Gross, Alain Makinson   +2 more
wiley   +1 more source

Mechanism of HIV-1 virion entrapment by tetherin.

PLoS Pathogens, 2013
Tetherin, an interferon-inducible membrane protein, inhibits the release of nascent enveloped viral particles from the surface of infected cells. However, the mechanisms underlying virion retention have not yet been fully delineated.
Siddarth Venkatesh, Paul D Bieniasz
doaj   +1 more source

The impact of accumulating immune adaptation in circulating strains of HIV‐1

HIV Medicine, EarlyView.
Abstract Background Mutations in human immunodeficiency virus type 1 (HIV‐1) enable the virus to evade recognition and killing by human leucocyte antigen (HLA)‐restricted T cells. These viral adaptations are specific to the HLA type of individuals and are therefore evident as HLA allele‐HIV sequence associations at the population level. Most studies of
Marwah Al‐kaabi, Alison Castley, Pooja Deshpande, Abha Chopra, David Nolan, Simon Mallal, Mina John, Silvana Gaudieri   +7 more
wiley   +1 more source

Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control

Nature Communications, 2018
Viral gene therapy can be limited by the efficacy of virion sequestration, immune responses and the silencing of genetic payloads. Here the authors engineer an advenovirus protein coat which shields the virion from the immune system while targeting ...
Markus Schmid, Patrick Ernst, Annemarie Honegger, Maarit Suomalainen, Martina Zimmermann, Lukas Braun, Sarah Stauffer, Cristian Thom, Birgit Dreier, Matthias Eibauer, Anja Kipar, Viola Vogel, Urs F. Greber, Ohad Medalia, Andreas Plückthun   +14 more
doaj   +1 more source

Fungal, not insect: Revisiting a misidentified Bemisia tabaci protein interacting with a begomovirus coat protein

Insect Molecular Biology, EarlyView.
A small heat‐shock protein (HSP16) previously reported as insect‐derived in Bemisia tabaci actually originates from a fungal species of the genus Wallemia. BLAST, genome survey and phylogenetic analyses support the fungal origin and clarify persistent misattribution in the literature.
Jesús Navas‐Castillo, Elvira Fiallo‐Olivé   +1 more
wiley   +1 more source

Development and Validation of a Viability RT‐qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV)

Journal of Fish Diseases, EarlyView.
ABSTRACT Spring viremia of carp virus (SVCV) is a contagious pathogen associated with significant mortality and economic losses in freshwater aquaculture. Although reverse transcription quantitative PCR (RT‐qPCR) enables rapid detection, it does not distinguish between infectious and non‐infectious viral particles, which may lead to overestimation of ...
Ha‐Jeong Son, Min‐Young Sohn, Jae‐Ok Kim, Hee Jung Choi, Mun‐Gyeong Kwon, Jeong‐Tae Lee, Gyoungsik Kang, HyeongJin Roh, Chan‐Il Park, Kyung‐Ho Kim   +9 more
wiley   +1 more source

The Role of Viruses in the Pathogenesis of Periodontitis

Journal of Periodontal Research, EarlyView.
This narrative review aimed to summarize current evidence on the presence and potential pathogenic role of viruses in periodontitis. Design: Narrative Review; Population: Patients with periodontitis; Exposure: Detection of viruses in oral samples (biopsies, saliva, gingival crevicular fluid, subgingival plaque, blood); Outcome: Viral prevalence, load ...
Kim Natalie Stolte, Jørgen Slots, Henrik Dommisch   +2 more
wiley   +1 more source

Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)-Seropositive Individuals Are Sites of HCMV Reactivation In Vivo. [PDF]

, 2015
Human cytomegalovirus (HCMV) causes significant morbidity in the immunocompromised host. Following primary infection, the virus establishes latent infection in progenitor cells of the myeloid lineage.

core   +1 more source

Beneficial rhizobacteria and virus infection modulate the soybean metabolome and influence the feeding preferences of the virus vector Epilachna varivestis

New Phytologist, EarlyView.
Summary Beneficial rhizobacteria and viral pathogens can both alter host plant phenotypes, yet little is known about how their simultaneous presence influences plant metabolism and species interactions. We investigated how two rhizobacteria, Bradyrhizobium japonicum and Delftia acidovorans, together with bean pod mottle virus (BPMV), shape soybean ...
Hannier Pulido, Kerry E. Mauck, Consuelo M. De Moraes, Mark C. Mescher   +3 more
wiley   +1 more source