Results 81 to 90 of about 7,003,490 (250)

Protocol for triggering plant gene silencing by viral delivery of short RNA

STAR Protocols
Summary: Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200–400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi).
Arcadio García, Verónica Aragonés, Paloma Ortiz-García, José-Antonio Daròs, Fabio Pasin   +4 more
doaj   +1 more source

Exploring plant genomes by RNA-induced gene silencing

, 2003
The nucleotide sequences of several animal, plant and bacterial genomes are now known, but the functions of many of the proteins that they are predicted to encode remain unclear.
Helliwell, C. A., Peter M. Waterhouse, Christopher A. Helliwell, Waterhouse, P. M.   +3 more
core   +1 more source

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

Advanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang, Qiang Chen, Aihua Jin, Xiaoqi Yang, Hui Zhou, Weiquan Gong, Jin Cheng, Jun Dai, Minjin Wang, Meihua Piao, Yuanyi Wang, Nan Zhou   +11 more
wiley   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source

Induction and maintenance of DNA methylation in plant promoter sequences by apple latent spherical virus-induced transcriptional gene silencing

Frontiers in Microbiology, 2014
Apple latent spherical virus (ALSV) is an efficient virus-induced gene silencing vector in functional genomics analyses of a broad range of plant species.
Tatsuya eKon, Nobuyuki eYoshikawa
doaj   +1 more source

Knocking down wheat meiotic genes through Virus-induced gene-silencing (VIGS)

, 2019
Virus-induced gene-silencing (VIGS) is a reverse genetic technology that can be used to assess gene function in model and crop species. The technique exploits a natural viral defence response in plants, whereby invading viral RNAs are detected, and this ...
Kanyuka, K., Higgins, J. D., Desjardins, S.   +2 more
core  

A Foxtail mosaic virus Vector for Virus-Induced Gene Silencing in Maize [PDF]

, 2016
Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (VIGS). A limited number of viruses have been developed into viral vectors for the purposes of gene expression or VIGS in monocotyledonous plants,
Kernodle, Bliss, Whitham, Steven, Mei, Yu, Hill, John, Zhang, Chunquan, Hill, John H, Whitham, Steven A, Kernodle, Bliss M   +7 more
core   +1 more source

Microglial Deubiquitinase OTUD7B Stabilizes STAT3 to Promote Neuroinflammation and Cognitive Decline in Alzheimer's Disease

Advanced Science, EarlyView.
. ABSTRACT Neuroinflammation driven by microglial activation is a defining feature of Alzheimer's disease (AD), yet the molecular mechanisms sustaining this proinflammatory state remain unclear. Here, we identify the deubiquitinase OTUD7B as a critical regulator of microglial activation and AD pathology.
Luyao Li, Hao Tang, Qin Yu, Yuyang Zhang, Binglu Shi, Ying Kong, Leiyu Xu, Jingjing Shao, Chenghong Hu, Lingyu She, Zhe Wang, Haiyi Chen, Aleksandr V. Samorodov, Gang Cao, Xia Zhao, Yi Wang   +15 more
wiley   +1 more source

Evaluation of Tobacco Rattle Virus-based Vectors for Gene Silencing and Editing in Tomato

Journal of the American Society for Horticultural Science
This study established an optimized protocol for tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) and virus-induced gene editing (VIGE) in tomato using phytoene desaturase (PDS) as a visual marker.
Hsin-Mei Ku, Jui-Chieh Liu, Shang-Ling Ou, Hui-Ju Chang, Sheng-Wen Wang, Ping-Yen Chiu, Guan-Ling Chen, Chien-Chi Huang, Chien-Hung Chung   +8 more
doaj   +1 more source

RNA duplexes in transcriptional regulation

Biomolecular Concepts, 2010
Transcriptional regulation by small RNA molecules, including small interfering RNA and microRNA, has emerged as an important gene expression modulator.
Swaminathan Sanjay, Hood Chantelle L., Suzuki Kazuo, Kelleher Anthony D.   +3 more
doaj   +1 more source