Results 111 to 120 of about 25,365 (278)

Oocyte–cumulus cell interaction: a key factor in early embryo development

Biological Reviews, EarlyView.
ABSTRACT The evaluation of oocyte competence is a fundamental step in achieving successful outcomes following assisted reproduction techniques (ART). At present, however, conventional oocyte maturation assessment is carried out by morphological observation, which is a subjective method that does not consider molecular features.
Marc Torres‐Garrido, Marc Yeste, Yentel Mateo‐Otero   +2 more
wiley   +1 more source

Effects of Early, High Moisture Harvesting on Grain, Milling, Flour, and Baking Quality of Six Winter Wheat Varieties

Cereal Chemistry, EarlyView.
ABSTRACT Background and Objectives Three US soft wheat varieties (Hilliard, Wilson, and Kokosing), one Korean soft wheat variety (Olgeru), and two Korean hard wheat varieties (Keumkang and Sukang) were harvested three times on weekly intervals beginning at approximately 20% grain moisture, and were evaluated for grain, milling, flour quality, and ...
Prabath Senanayaka Mudiyanselage, Jinhee Park, Byung‐Kee Baik, Laura E. Lindsey   +3 more
wiley   +1 more source

Perbandingan Morfologi Antara Oosit Sapi yang di Vitrifikasi Sebelum dan Sesudah Maturasi In Vitro = The Comparison of Morphology Between Bovine Oocyte Vitrification Before and After In Vitro Maturation

, 2014
Vitrification method has become a growing and promising technology in assisted reproductive technology. Freezing embryos are more common, freezing oocytes are longer growing because the physical characteristics of oocyte.
Faizah, Zakiyatul, Hinting, Aucky, Widjiati, Darsini, Ninik   +3 more
core  

Bulk droplet vitrification for primary hepatocyte preservation

, 2019
Vitrification is a promising ice-free alternative for classic slow-freezing (at approximately 1 °C/min) cryopreservation of biological samples. Vitrification requires extremely fast cooling rates to achieve transition of water into the glass phase while ...
Ozer, Sinan, Uygun, Korkut, Banik, Peony D., Weng, Lindong, Nagpal, Sonal, Tessier, Shannon N., de Vries, Reinier J., Toner, Mehmet, van Gulik, Thomas M.   +8 more
core   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

Production and Purification of Mesenchymal Stem Cell‐Derived Extracellular Vesicles Using Preferential Exclusion Chromatography

ELECTROPHORESIS, EarlyView.
A scalable purification strategy using hydroxyl (OH) monolithic chromatography enables efficient isolation and direct concentration of MSC‐EVs from adherent and microcarrier‐based upstreams. This approach supports process scale‐up while ensuring high purity suitable for therapeutic EV manufacturing.
Katja Vrabec, Andrej Raspor, Darja Božič, Nastja Bizjak Ternik, Ana Železnik, Valentina Novak, Ivana Petrović Koshmak, Maja Leskovec, Aleš Štrancar   +8 more
wiley   +1 more source

The role of heat shock protein 70 on oocyte apoptosis during vitrification

Open Veterinary Journal
The cryopreservation of animal and human oocytes has potential for the development of Assisted Reproductive Technology (ART), especially as bank of oocytes.
Rimayanti Rimayanti, Aswin Rafif Khairullah, Sri Pantja Madyawati, Fedik Abdul Rantam, Imam Mustofa, Widjiati Widjiati, Pudji Srianto, Adeyinka Oye Akintunde, Bima Putra Pratama, Riza Zainuddin Ahmad, Bantari Wisynu Kusuma Wardhani, Andi Thafida Khalisa, Syahputra Wibowo   +12 more
doaj   +1 more source

Phosphate and fluoride removal options for Hanford high‐level waste sludge processing

Environmental Progress &Sustainable Energy, EarlyView.
Abstract High‐phosphate and high‐fluoride sludges represent some of the most challenging waste streams within the Hanford High‐Level Waste (HLW) inventory due to solubility constraints, impacts on glass loading, and corrosion risks during vitrification.
A. M. Westesen, K. J. Murray, A. A. Bachman, A. M. Robb, R. A. Peterson   +4 more
wiley   +1 more source

Cryo-FIB Lift-Out and Electron Tomography Workflow for Bacteria-Nanopillar Interface Imaging Under Native Conditions: Investigating Dragonfly Inspired Bactericidal Titanium Surfaces. [PDF]

Small Methods
A correlative cryo‐microscopy workflow enables targeted extraction and cryo‐electron tomography of bacteria–nanopillar interfaces under fully hydrated, near‐native conditions. By overcoming limitations of conventional imaging, this approach allows direct structural interrogation of bacteria–nanostructure interactions in situ, providing a methodological
Bandara CD, Pinkas D, Zanova M, Uher M, Mantell J, Su B, Nobbs AH, Verkade P.   +7 more
europepmc   +2 more sources

Microencapsulation of Lactobacillus helveticus L‐6 by Electrostatic Spray Drying: Efficient Preparation and Characterization Analysis

Food Bioengineering, EarlyView.
Superior survival & stability: electrostatic spray drying (ESD) achieved 82% initial viability, retaining 79% survival after 3‐month at 4°C storage. Optimized protection: 70°C drying with resistant dextrin & whey protein preserved 93.5% membrane integrity.
Xiao‐Ting Ye, Yin Zhang, Hao‐Lun Huang, Xin Li, Hong‐Yu Xu, Li‐Juan Chai, Xiao‐Juan Zhang, Jin‐Song Shi, Zhen‐Ming Lu, Zheng‐Hong Xu   +9 more
wiley   +1 more source