We present a fully automated, unattended cryo‐EM pipeline that serves as an on‐the‐fly diagnostic tool, enabling rapid data‐quality assessment and informed decision‐making to maximize data‐collection efficiency.Single‐particle analysis (SPA) by cryogenic electron microscopy (cryo‐EM) has become a cornerstone of structural biology; yet, the workflow ...
Daniel Marchán Torres +14 more
wiley +1 more source
Single-cell RNA and DNA methylome profiling reveal liquid helium vitrification enhances porcine parthenogenetically activated blastocyst viability. [PDF]
Ayantoye JO +8 more
europepmc +1 more source
Reproductive outcomes in women with Hodgkin's lymphoma in a Danish fertility preservation cohort
The risk of premature ovarian insufficiency increases with the intensity of alkylating chemotherapy. In general, fertility outcomes were favorable, but chances of live birth decreased with increased intensity of alkylating chemotherapy. Ovarian tissue transplantation enabled natural conception in women with premature ovarian insufficiency, supporting ...
Lotte Berdiin Colmorn +4 more
wiley +1 more source
The evolution of liver graft preservation strategies in liver transplantation. [PDF]
Gao W +9 more
europepmc +1 more source
Dehydration‐driven organization of metabolites into NaDES‐like assemblies in orthodox seeds
Summary Desiccation tolerance in plants, especially during orthodox seed dehydration, relies on compatible solute accumulation, complex molecular mechanisms, and intermolecular organizations that remain poorly understood. We combined metabolite profiling, mass spectrometry imaging, and electrospray and cold‐spray ionization mass spectrometry to ...
Youcef Haddad +11 more
wiley +1 more source
Development of a three-dimensional vitrification protocol for domestic cat cumulus-oocyte complexes and comparison with standard vitrification. [PDF]
Colombo M +8 more
europepmc +1 more source
Backside thinning techniques critically influence GaN laser performance. Compared to lapping, grinding reduces subsurface damage depth fivefold and lowers wafer bow, while mechanical polishing eliminates subsurface damage entirely. These improvements directly translate to smoother cleaved facets and a nearly 40% reduction in laser threshold current ...
S. Makhladi +6 more
wiley +1 more source
Supplementing Coenzyme Q10 During the Vitrification and In Vitro Maturation of Dromedary Camel Oocytes Significantly Enhances Their Developmental Competence. [PDF]
Yaqout KA +4 more
europepmc +1 more source
Effect of Hydroxyapatite Nanoparticles on the Ultrastructure, Developmental Competence, and Expression of <i>ZP3</i>, <i>MFN1</i>, and <i>NPM2</i> in Vitrified Bovine GV Oocytes. [PDF]
Li XX +13 more
europepmc +1 more source
Functional recovery of the adult murine hippocampus after cryopreservation by vitrification. [PDF]
German A +8 more
europepmc +1 more source

