Results 221 to 230 of about 10,863,365 (261)

Volume electron microscopy reveals placental ultrastructure in 3D

Placenta, 2023
Many structures necessary for placental function can only be visualised at the ultrastructural scale. Recent technological advances have made Volume electron microscopy (volume EM) approaches much more accessible. Volume EM allows the ultrastructure of tissues, cells and organelles to be visualised in 3D.
Rohan Lewis
exaly   +3 more sources

Some of the next articles are maybe not open access.

Related searches:

Author Correction: Volume electron microscopy

Nature Reviews Methods Primers, 2022
In the version of this article initially published, the orientation of the bridge appearing below the dashed arrow in the “FIB- SEM/pFIB- SEM” section of Fig. 2 was incorrect, and article titles or journal listings for refs. 3, 42, 45, 155, 160, 175 and 197 were omitted.
Christopher J. Peddie, Christel Genoud, Anna Kreshuk, Kimberly Meechan, Kristina D. Micheva, Kedar Narayan, Constantin Pape, Robert G. Parton, Nicole L. Schieber, Yannick Schwab, Benjamin Titze, Paul Verkade, Aubrey Weigel, Lucy M. Collinson   +13 more
openaire   +3 more sources

Whole-cell organelle segmentation in volume electron microscopy

Nature, 2021
Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a complete understanding of their intricate organization requires the nanometre-level, three-dimensional reconstruction of whole cells, which is only feasible with robust and scalable automatic methods.
Larissa Heinrich, Davis Bennett, David Ackerman, Woohyun Park, John Bogovic, Nils Eckstein, Alyson Petruncio, Jody Clements, Song Pang, C. Shan Xu, Jan Funke, Wyatt Korff, Harald F. Hess, Jennifer Lippincott-Schwartz, Stephan Saalfeld, Aubrey V. Weigel, Riasat Ali, Rebecca Arruda, Rohit Bahtra, Destiny Nguyen   +19 more
openaire   +2 more sources

Physical basics of scanning electron microscopy in volume electron microscopy

Microscopy
Abstract Volume electron microscopy (vEM) has become a widely adopted technique for acquiring three-dimensional structural information of biological specimens. In addition to the traditional use of transmission electron microscopy, recent advances in the resolution of scanning electron microscopy (SEM) made it suitable for vEM ...
Mitsuo, Suga, Yusuke, Hirabayashi
openaire   +2 more sources

Volume scanning electron microscopy for imaging biological ultrastructure

Biology of the Cell, 2016
Electron microscopy (EM) has been a key imaging method to investigate biological ultrastructure for over six decades. In recent years, novel volume EM techniques have significantly advanced nanometre‐scale imaging of cells and tissues in three dimensions.
Benjamin Titze, Christel Genoud
exaly   +4 more sources

Serial block face-scanning electron microscopy for volume electron microscopy

2019
There are different technologies that can be used to obtain a 3D image at nanometer resolution. Over the past decade, there has been a growing interest in applying Serial Block Face Scanning Electron Microscopy (SBF-SEM) in different fields of life science research.
Saskia, Lippens, Anna, Kremer, Peter, Borghgraef, Christopher J, Guérin   +3 more
openaire   +2 more sources

Automated synaptic connectivity inference for volume electron microscopy

Nature Methods, 2017
Teravoxel volume electron microscopy data sets from neural tissue can now be acquired in weeks, but data analysis requires years of manual labor. We developed the SyConn framework, which uses deep convolutional neural networks and random forest classifiers to infer a richly annotated synaptic connectivity matrix from manual neurite skeleton ...
Joergen Kornfeld, Gregor Urban, Philipp J Schubert   +2 more
exaly   +5 more sources

A wide variety of techniques for a volume electron microscopy

Microscopy
Abstract Electron microscopy (EM) is known to be the only research equipment able to resolve the ultrastructure of cells, including intracellular organelles and synapses. Researchers studying the brain connectome have re-evaluated the value of EM. The development of new EM techniques and tools has been active in these two decades.
Yoshiyuki, Kubota, Takaaki, Miyazaki, Nilton L, Kamiji, Tamami, Honda, Motohide, Murate, Mitsuo, Suga   +5 more
openaire   +2 more sources

Preparation of Retinal Samples for Volume Electron Microscopy

Journal of Visualized Experiments
Volume electron microscopy (Volume EM) has emerged as a powerful tool for visualizing the 3D structure of cells and tissues with nanometer-level precision. Within the retina, various types of neurons establish synaptic connections in the inner and outer plexiform layers.
Wenna, Zhao, Qingwen, Yang, Xiaoqian, Lai, Jun, Zhang   +3 more
openaire   +2 more sources

A conventional fixation volume electron microscopy protocol for plants

2023
Volume electron microscopy techniques play an important role in plant research from understanding organelles and unicellular forms to developmental studies, environmental effects and microbial interactions with large plant structures, to name a few. Due to large air voids central vacuole, cell wall and waxy cuticle, many plant tissues pose challenges ...
Janithri S, Wickramanayake, Kirk J, Czymmek   +1 more
openaire   +2 more sources