Results 61 to 70 of about 10,863,365 (261)
Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
Molecular Oncology, EarlyView.How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source
Volume electron microscopy for genetically and molecularly defined neural circuits
Neuroscience ResearchThe brain networks responsible for adaptive behavioral changes are based on the physical connections between neurons. Light and electron microscopy have long been used to study neural projections and the physical connections between neurons.
Nobuhiko Ohno +2 more
doaj +1 more source
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System
Frontiers in Neural Circuits, 2018 The “connectome,” a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al.
Ben Mulcahy +12 more
doaj +1 more source
Molecular Oncology, EarlyView.This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Applied MicroscopyWe established a correlative workflow combining X-ray microscopy (XRM) and transmission electron microscopy (TEM) to investigate the ultrastructure of zebrafish neuromasts.
Sun-Yeong Gwon, MinKi Choi, Ji Young Mun
doaj +1 more source
Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis.
PLoS Pathogens, 2020 New techniques for obtaining electron microscopy data through the cell volume are being increasingly utilized to answer cell biologic questions. Here, we present a three-dimensional atlas of Plasmodium falciparum ultrastructure throughout parasite cell ...
Rachel M Rudlaff +3 more
doaj +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster [PDF]
Cell, 2017 SUMMARY Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000–neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain ...
Zheng, Zhihao +22 more
openaire +2 more sources