Results 71 to 80 of about 43,106 (210)

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

open access: yesJournal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb   +6 more
wiley   +1 more source

Sequence analysis of the VP2 gene hypervariable region of infectious bursal disease viruses from India [PDF]

open access: yesAvian Pathology, 2001
Attempts have been made to characterize infectious bursal disease virus (IBDV) isolates collected from different parts of India during 1993 to 1999. Phylogenetic analysis was performed on a sequence generated by cycle sequencing comprising the variable region of the VP2 gene of 14 isolates.
R S, Kataria   +4 more
openaire   +2 more sources

Chicken IL-17 as a potent adjuvant enhances IBDV VP2 DNA vaccine immunogenicity and protective efficacy

open access: yesPoultry Science
Infectious bursal disease virus (IBDV) is an important immunosuppressive virus in chickens, which caused an acute and highly contagious infectious disease and resulted in considerable economic losses in the world poultry industry.
Jianlou Zhang   +4 more
doaj   +1 more source

Ion Activation Methods for Top‐Down Proteomics

open access: yesMass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Prevalence of Eight Viruses in Captive Amur Tigers (Panthera tigris altaica) From Harbin, China 中国哈尔滨市圈养东北虎8种病毒的流行

open access: yesWildlife Letters, EarlyView.
The Amur tiger, an endangered flagship species mainly found in the Russia Far East and Northeastern China, holds immense biological importance, and has been listed under the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES).
Wei Xie   +15 more
wiley   +1 more source

Iflaviruses in arthropods: when small is mighty

open access: yesInsect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia   +3 more
wiley   +1 more source

First identification of canine parvovirus ‐2a/2b variant in unvaccinated domestic dogs with gastrointestinal signs in Türkiye

open access: yesVeterinary Medicine and Science
Background Canine parvovirus type 2 (CPV‐2) is the most common enteric virus that infects canids. CPV is the causative agent of a contagious disease defined mostly by clinical gastrointestinal signs in dogs.
Hasbi Sait SALTIK, B Taylan KOÇ
doaj   +1 more source

Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

open access: yesFrontiers in Microbiology, 2022
The fact that wild felines are carriers of pernicious infectious viruses should be a major concern due to the potential cross-species transmission between the felines and human or domestic animals.
Shuping Huang   +12 more
doaj   +1 more source

The immunogenicity of recombinant vaccines based on modified Vaccinia Ankara (MVA) viruses expressing African horse sickness virus VP2 antigens depends on the levels of expressed VP2 protein delivered to the host [PDF]

open access: yes, 2018
African horse sickness (AHS) is a lethal equine disease transmitted by Culicoides biting midges and caused by African horse sickness virus (AHSV). AHS is endemic to sub-Saharan Africa, but devastating outbreaks have been recorded periodically outside ...
Calvo-Pinilla, Eva   +4 more
core   +2 more sources

Expanding the Fish‐Brain Invitrome With the Senegalese Sole SsB‐1 Cell Line—A Versatile Model for Neurotropic Virus Research

open access: yesJournal of Fish Diseases, EarlyView.
ABSTRACT Cell lines are essential tools for studying animal physiology and immunopathology, reducing the use of live animals and supporting the 3Rs principle of reduction, refinement, and replacement. The Senegalese sole (Solea senegalensis), a relevant species for Spanish aquaculture diversification, lacks established in vitro models. We developed SsB‐
Yulema Valero, Alberto Cuesta
wiley   +1 more source

Home - About - Disclaimer - Privacy