Results 71 to 80 of about 36,678 (201)

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 7, Page 1384-1395, July 2026.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies

Journal of Immunology Research, 2014
Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and
Paulius Lukas Tamošiūnas, Rasa Petraitytė-Burneikienė, Rita Lasickienė, Artiomas Akatov, Gabrielis Kundrotas, Vilimas Sereika, Raimundas Lelešius, Aurelija Žvirblienė, Kęstutis Sasnauskas   +8 more
doaj   +1 more source

Exchange of the C-terminal part of VP3 from very virulent infectious bursal disease virus results in an attenuated virus with a unique antigenic structure

, 2002
Infectious bursal disease virus (IBDV) is the major viral pathogen in the poultry industry. Live attenuated serotype 1 vaccine strains are commonly used to protect susceptible chickens during their first 6 weeks of life. Wild-type serotype 1 IBDV strains
Peeters, B.P.H., ter Huurne, A.A.H.M., Pol, J.M., Hoekman, A.J.W., Huurne, A.A.H.M., ter, Boot, H.J., Gielkens, A.L.J.   +6 more
core   +1 more source

Fatal Myocarditis Caused by Canine Parvovirus in a Litter of One‐Day‐Old German Shepherd Puppies: Molecular and Histopathological Findings

Veterinary Medicine and Science, Volume 12, Issue 4, July 2026.
Canine parvovirus infection resulted in acute fatal myocarditis in one‐day‐old German Shepherd puppies. Histopathology demonstrated lymphoplasmacytic myocarditis with intranuclear inclusion bodies, and PCR confirmed viral DNA in cardiac tissue. These findings indicate perinatal transmission and emphasise that CPV myocarditis remains a lethal threat to ...
Seyedhosein Jarolmasjed, Mohammadreza Ghorani, Amir Ali Shahbazfar, Hamed Sepehr, Seyed Hossein Zamzam, Sina Soleimani   +5 more
wiley   +1 more source

Phylogenetic analysis of VP2 gene of canine parvovirus and comparison with Indian and world isolates

Acta virologica, 2016
Canine parvovirus (CPV) causes hemorrhagic enteritis, especially in young dogs, leading to high morbidity and mortality. It has four main antigenic types CPV-2, CPV-2a, CPV-2b and CPV-2c. Virus protein 2 (VP2) is the main capsid protein and mutations affecting VP2 gene are responsible for the evolution of various antigenic types of CPV. Full length VP2
G, Kaur, M, Chandra, P N, Dwivedi
openaire   +3 more sources

A Mechanistic Model of rAAV Production in Synthetic Cell Lines

Biotechnology and Bioengineering, Volume 123, Issue 6, Page 1684-1694, June 2026.
ABSTRACT The recombinant adeno‐associated virus (rAAV) is a widely used vector for gene therapy. Its manufacturing faces significant challenges in producing the large quantities of vectors needed for clinical applications and reducing empty particles.
Prahalad Srinivasan, Han‐Jung Kuo, Yu‐Chieh Lin, Yen‐An Lu, Wei‐Shou Hu, Qi Zhang   +5 more
wiley   +1 more source

Applying Distinct CDMS Strategies to Observe Nonclassical Virus Capsid Assembly

Journal of Mass Spectrometry, Volume 61, Issue 6, June 2026.
ABSTRACT In conventional native mass spectrometry (MS), one faces severe limitations when challenged with heterogeneous, high‐mass samples, commonly failing to resolve clear peak distributions, and thus mass determination. Charge detection MS (CDMS) has emerged as a premier method to analyze these samples by determining mass‐to‐charge ratio (m/z) and ...
Lars Thiede, Anisha Haris, Tomislav Damjanović, Jocky C. K. Kung, Jürgen Müller‐Guhl, Ronja Pogan, Jennifer Rothe, Wiebke Schultze, Sanne Ugelstad, David Eatough, Kevin Giles, Steve Preece, Keith Richardson, Jakub Ujma, Charlotte Uetrecht   +14 more
wiley   +1 more source

Organoids: From Bench to Bedside Applications

MedComm, Volume 7, Issue 6, June 2026.
Organoids, as a groundbreaking biomedical research platform, utilize adult stem cells (ASCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs) as sources. By integrating specific growth and differentiation signals within an extracellular matrix (e.g., Matrigel), organoids guide cells to self‐assemble into three‐dimensional ...
Kelin Li, Rui Cao, Maochen Li, Zichao Tian, Huahao Fan, Bixia Hong, Xiaojuan Liu   +6 more
wiley   +1 more source

Nucleotide sequence of the gene encoding for the RNA binding protein (VP2) of RF bovine rotavirus

Nucleic Acids Research, 1989
International ...
Kumar, A., Charpilienne, Annie, Cohen, J.   +2 more
openaire   +4 more sources

Nanobodies: A Promising Toolkit for Diagnostic Applications

SmartMat, Volume 7, Issue 3, June 2026.
This review focuses on camelid‐derived nanobodies (VHHs) and explains how their small size and high stability support robust diagnostic design. Applications across ELISA, lateral flow assays, and PET/SPECT imaging are summarized, along with clinical progress such as caplacizumab.
Wei Wu, Abid Ali, Li Sun, Jiahao Hu, Lina Hameed, Saba Shafi, Ali Raza, Khuzin Dinislam, Wenjing Xing, Long Rong, Jie Lin, Jian Ma   +11 more
wiley   +1 more source