Results 101 to 110 of about 1,248,351 (293)

Production of polyclonal antiserum against beet western yellows virus coat protein expressed in Escherichia coli

Journal of Crop Protection, 2015
Serological methods are commonly used methods for detection of viruses. Preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies.
Arezu Lotfi, Masoud Shams-Bakhsh, Bagher Yakhchali   +2 more
doaj  

The detection of C/EBPβ expression in the lesions of patients with psoriasis vulgaris by Western blot

Pifu-xingbing zhenliaoxue zazhi, 2019
Objective: To detect the expression of C/EBPβ protein in the lesions of patients with psoriasis vulgaris by western blot. Methods: C/EBPβ protein expression levels of tissues from 15 psoriatic lesions and 10 healthy controls were determined by the means ...
Li-dan LIU, Xiao-hua WANG, Jia-rui LIU, Yong-feng CHEN   +3 more
doaj   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

IgG1 and IgG2a Profile of Serum Antibodies to Leishmania major Amastigote in BALB/c and C57BL/6Mice

Iranian Journal of Allergy, Asthma and Immunology, 2013
It is well accepted that in experimental model of Leishmania major infection, BALB/c mice mount a Th2 response and produce IgG1 predominantly whereas C57BL/6mice enhance Th1 response with the biased production of IgG2a antibodies.
Sedighe Ebrahimpoor, Saeed-Reza Pakzad, Soheila Ajdary   +2 more
doaj  

Preparation of Parasite Protein Extracts and Western Blot Analysis

Bio-Protocol, 2014
In order to prepare protein extracts of Plasmodium falciparum blood stages for western blot analysis, infected red blood cells (iRBC) need to be separated from uninfected red blood cells (uRBC) which make up the bulk of the parasite culture. Depending on
Arlett Heiber, Tobias Spielmann
doaj   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source

Western Blot Analysis of Chloroplast HSP70B in Chlorella Species

Bio-Protocol, 2013
Western blotting allows for the specific detection of proteins by an antibody of interest. This protocol utilizes isolation of total proteins protocol for Chlorella vulgaris prior to gel electrophoresis. After electrophoresis, the selected antibodies are
Stephka Chankova, Zhana Mitrovska, Nadezhda Yurina   +2 more
doaj   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Western blot detection of PMI protein in transgenic rice

Journal of Integrative Agriculture, 2016
Phosphomannose isomerase (PMI) encoding gene manA is a desirable selective marker in transgenic research. Understanding of its expression patterns in transgenic plant and establishing highly sensitive detection method based on immunoassay have great ...
Rui-juan RONG, Peng-cheng WU, Jin-ping LAN, Han-fu WEI, Jian WEI, Hao CHEN, Jia-nan SHI, Yu-jie HAO, Li-juan LIU, Shi-juan DOU, Li-yun LI, Lin WU, Si-qi LIU, Chang-cheng YIN, Guo-zhen LIU   +14 more
doaj   +1 more source