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Hybridoma Screening by Antibody Capture: A High-Throughput Western Blot Assay
Cold Spring Harbor Protocols, 2021Antibody capture assays are often the easiest and most convenient of the hybridoma screening methods. In this procedure, proteins in solution or in a cell lysate are separated according to size by gel electrophoresis and then transferred by blotting to a nitrocellulose sheet.
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Rapid Real‐Time Ubiquitination Assays as Alternatives to Western Blotting
The FASEB Journal, 2020The refolding and degradative pathways of the protein quality control system converge through the chaperoned ubiquitination complex formed by the interaction of the E3 ubiquitin ligase CHIP ( C ‐terminus of H
Ruvindi de Silva, Richard C. Page
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A photodetection device for luminol-based immunodot and Western blotting assays
Analytical Biochemistry, 1988A photodetection device permitting rapid and direct photographic recording of light emission from luminol-based immunodot and Western blotting assays is described in detail. This device permits contact exposure on Polaroid film of luminescent samples on nitrocellulose strips, and thus obviates the need for darkroom and film processing facilities ...
M M, Leong, G R, Fox, J S, Hayward
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Enhancement of luminol-based immunodot and Western blotting assays by iodophenol
Analytical Biochemistry, 1988The application of 4-iodophenol as a signal enhancer for luminol-based immunodot and Western blotting assays was investigated. Immunodetection of biotin-bovine serum albumin (BSA) using a rat anti-biotin antibody for immunodot binding and goat anti-biotin peroxidase conjugate for Western blotting assays was employed to demonstrate the signal ...
M M, Leong, G R, Fox
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Densitometric analysis of Western blot assays for feline immunodeficiency virus antibodies
Veterinary Immunology and Immunopathology, 2001Western blot (WB) strips for antibodies directed to feline immunodeficiency virus (FIV) were analysed using reflectance densitometry by a semiautomatic densitometer. This method was used to quantify the antibody responses to different FIV proteins in both vaccinated and naturally or experimentally-infected cats.
Calandrella M +3 more
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Clinical Diagnostic Laboratory Immunology, 1997
We tested 101 serum samples obtained from pregnant women for the presence of human cytomegalovirus (HCMV)-specific IgM with nine different commercially available kits and with two Western blotting (WB) tests previously developed in our laboratory.
T, Lazzarotto +3 more
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We tested 101 serum samples obtained from pregnant women for the presence of human cytomegalovirus (HCMV)-specific IgM with nine different commercially available kits and with two Western blotting (WB) tests previously developed in our laboratory.
T, Lazzarotto +3 more
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New anti‐human immunodeficiency virus immunoblot assays resolve nonspecific western blot results
Transfusion, 1997BACKGROUND: Two new anti‐human immunodeficiency virus type 1 and 2 (HIV‐ 1/2) immunoblot assays, which use recombinant and synthetic antigens for antibody detection have become available. These assays might be able to resolve indeterminate anti‐HIV Western blot results.
H L, Zaaijer +4 more
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Validation of microRNA Target Genes Using Luciferase Reporter assay and Western Blot Analysis
2022MicroRNA s regulate gene expression by binding to the 3'untranslated region (UTR) of the mRNA of their target genes. Identification of microRNA target genes enables the determination of their functional role in the cells. A single microRNA can target multiple genes, all of which have a microRNA binding site in their 3' UTR. Putative target genes can be
Pooja, Panwalkar +2 more
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HIV antibodies in whole saliva detected by ELISA and western blot assays
Journal of Medical Virology, 1990AbstractPaired serum and saliva samples were tested by enzyme‐linked immunosorbent assay (ELISA) and Western blot (WB) for the presence of human immunodeficiency virus (HIV) antibodies. The study group included 36 individuals known to be HIV seropositive and 14 healthy, seronegative controls.
P, Holmström +4 more
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A new Western blot assay for the detection of porcine cytomegalovirus (PCMV)
Journal of Immunological Methods, 2016Porcine cytomegalovirus (PCMV) may be harmful for human recipients if xenotransplantation using pig cell, tissue or organ will be performed transmitting the virus from donor pigs to human recipients. PCMV is widespread in pigs and closely related to human pathogenic herpesviruses, however there are no data concerning infection of humans.
Elena, Plotzki +3 more
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