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Western Blot Assay for Quantitative and Qualitative Antigen Detection in Vaccine Development

Current Protocols in Microbiology, 2014
AbstractImmunological methods for quantitative measurement, antigenic characterization, and monitoring the stability of active immunogenic component(s) are a critical need in the vaccine development process. This unit describes an enhanced chemiluminescence‐based western blot for quantitative detection of Plasmodium falciparum circumsporozoite protein (
Sanjai, Kumar   +5 more
openaire   +2 more sources

A western blot assay detects autoantibodies to cryptic endothelial antigens in thrombotic microangiopathies

Journal of Clinical Immunology, 1993
Autoantibodies detected by immunofluorescence, ELISA, and complement-fixation techniques have provided discriminatory markers for many human diseases. However, these commonly applied assays may fail to detect antibodies against antigenic sites which are either inaccessible or not displayed in recognizable cellular structures.
D W, Koenig   +2 more
openaire   +2 more sources

A chemiluminescent-western blot assay for quantitative detection of Plasmodium falciparum circumsporozoite protein

Journal of Immunological Methods, 2013
Highly sensitive and reliable assays based on the quantitation of immunologically relevant component(s) in recombinant or whole parasite-based vaccines would facilitate pre-clinical and clinical phases and the monitoring of malaria vaccine deployment.
Sanjai, Kumar   +8 more
openaire   +2 more sources

Rapid and efficient western blot assay by rotational cyclic draining and replenishing procedure

ELECTROPHORESIS, 2018
AbstractWestern blot is a principal technique for the detection of specific proteins in various biology disciplines. The antibody incubation is an imperative step in western blot. Antibody incubation by mild shaking on a rocker is generally used to facilitate mixing of antibodies.
Saba Sajjad   +4 more
openaire   +2 more sources

Radioimmune western blotting in comparison with conventional western blotting, second and third generation ELISA assays for the serodiagnosis of HIV-1 infection.

The new microbiologica, 1994
We compared the performance of second and third generation ELISA assays to detect antibodies to HIV-1 virus with conventional Western blotting (WB) and radioimmune Western blotting (RIWB). Both sera from commercial seroconversion panels and serial dilutions of a serum for HIV-1 antibodies were tested with Murex HIV Recombinant, Vidas bioMérieux HIV 1/2
PORTINCASA, Pietro   +4 more
openaire   +1 more source

Absence of HIV Infection in Blood Donors with Indeterminate Western Blot Tests for Antibody to HIV-1

New England Journal of Medicine, 1990
J Brooks Jackson, Susan L Stramer
exaly  

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