Results 91 to 100 of about 2,778,272 (379)

A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

BMC Research Notes, 2012
Background We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments.
Dimauro Ivan, Pearson Timothy, Caporossi Daniela, Jackson Malcolm J   +3 more
doaj   +1 more source

Regional distribution of the prostaglandin E2 receptor EP1 in the rat brain: accumulation in Purkinje cells of the cerebellum [PDF]

, 2005
Prostaglandin E2 (PGE2), is a major prostanoid produced by the activity of cyclooxygenases (COX) in response to various physiological and pathological stimuli. PGE2 exerts its effects by activating four specific E-type prostanoid receptors (EP1, EP2, EP3
Akundi, Ravi S., Candelario-Jalil, Eduardo, Fiebich, Bernd L., Hull, Michael, Ridelis, Ingrid, Slawik, Helen, Waschbisch, Anne   +6 more
core  

Interaction specificity of Arabidopsis 14-3-3 proteins with phototropin receptor kinases [PDF]

, 2009
Phototropin receptor kinases play an important roles in optimising plant growth in response to blue light. Much is known regarding their photochemical reactivity, yet little progress has been made to identify downstream signalling components.
Bachmann, Blakeslee, Brown, Catriona E. Thomson, Cho, Christie, Christie, Christie, Christie, Cutler, Delille, DeLong, Eirini Kaiserli, Fuglsang, Gebbie, Gendron, Huala, Inada, Inoue, Inoue, John M. Christie, Kagawa, Kim, Kinoshita, Kinoshita, Kinoshita, Kong, Lariguet, Matsuoka, Motchoulski, Nakasako, Page, Pedmale, Rosenquist, Sakamoto, Schoonheim, Sehnke, Steinmann, Stuart Sullivan, Sullivan, Takemiya, Thompson, Wade, Yan   +43 more
core   +1 more source

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source

Subcellular western blotting of single cells

Microsystems & Nanoengineering, 2017
Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors.
K. Yamauchi, A. Herr
semanticscholar   +1 more source

Analysis of tissue transglutaminase function in the migration of swiss 3T3 fibroblasts - the active-state conformation of the enzyme does not affect cell motility but is important for its secretion [PDF]

, 2002
Increasing evidence suggests that tissue transglutaminase (tTGase; type II) is externalized from cells, where it may play a key role in cell attachment and spreading and in the stabilization of the extracellular matrix (ECM) through protein cross-linking.
Achyuthan, Aeschlimann, Aeschlimann, Akimov, Akiyama, Ball, Barsigian, Bowness, Bowness, Brown, Cai, Cariello, Di Venere, Fogerty, Folk, Freund, Gaudry, Gaudry, Gentile, Gossen, Griffin, Haroon, Isobe, Jackson, Jeong, Johnson, Johnson, Johnson, Jones, Laemmli, Lee, Lorand, Lowry, Martinez, Mian, Miyakawa, Palecek, Piotrowicz, Smethurst, Stewart, Tarantini, Upchurch, Verderio, Verderio, Weiss   +44 more
core   +1 more source

Response to neoadjuvant chemotherapy in early breast cancers is associated with epithelial–mesenchymal transition and tumor‐infiltrating lymphocytes

Molecular Oncology, EarlyView.
Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane, Jérôme Ambroise, Cédric van Marcke, Mieke Van Bockstal, Martine Berlière, Christine Galant, Hélène Dano, Médina Lougué, Elena Benidovskaya, Guy Jerusalem, Vincent Bours, Claire Josse, Jérôme Thiry, Aurélie Daumerie, Caroline Bouzin, Cyril Corbet, François P. Duhoux   +16 more
wiley   +1 more source

Modified Western blotting for insulin and other diabetes-associated peptide hormones

Scientific Reports, 2017
Now, the quantification of proinsulin/insulin contents within organisms tends to be evaluated only by enzyme-linked immunosorbent assay (ELISA), although assessing the adequacy of results by some quantification method is important.
Naoyuki Okita, Y. Higami, F. Fukai, Masaki Kobayashi, Miku Mitarai, T. Sekiya, Takashi Sasaki   +6 more
semanticscholar   +1 more source

TOMM20 as a driver of cancer aggressiveness via oxidative phosphorylation, maintenance of a reduced state, and resistance to apoptosis

Molecular Oncology, EarlyView.
TOMM20 increases cancer aggressiveness by maintaining a reduced state with increased NADH and NADPH levels, oxidative phosphorylation (OXPHOS), and apoptosis resistance while reducing reactive oxygen species (ROS) levels. Conversely, CRISPR‐Cas9 knockdown of TOMM20 alters these cancer‐aggressive traits.
Ranakul Islam, Megan E. Roche, Zhao Lin, Diana Whitaker‐Menezes, Victor Diaz‐Barros, Eurico Serrano, Maria Paula Martinez Cantarin, Nancy J. Philp, Atrayee Basu Mallick, Ubaldo Martinez‐Outschoorn   +9 more
wiley   +1 more source

A Proteomics Approach to the Protein Normalization Problem: Selection of Unvarying Proteins for MS-Based Proteomics and Western Blotting.

Journal of Proteome Research, 2016
Proteomics and other protein-based analysis methods such as Western blotting all face the challenge of discriminating changes in the levels of proteins of interest from inadvertent changes in the amount loaded for analysis.
J. Wiśniewski, M. Mann
semanticscholar   +1 more source