INTRODUCTION: Snake envenomings are a health problem in rural areas of tropical and subtropical countries, but little is known regarding the immune response presented by bitten individuals.
Karla Patricia Oliveira Luna +4 more
doaj +1 more source
Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing [PDF]
The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously.
Bulleid, Neil J., Cao, Zhenbo
core +1 more source
Glutamate induces autophagy via the two-pore channels in neural cells [PDF]
NAADP (nicotinic acid adenine dinucleotide phosphate) has been proposed as a second messenger for glutamate in neuronal and glial cells via the activation of the lysosomal Ca2+ channels TPC1 and TPC2.
  +11 more
core +4 more sources
Seal parapoxvirus (SPPV) infection has been reported among pinnipeds in aquaria in Japan; however, its seroprevalence is unknown. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of SPPV infection.
Badr Yassien +9 more
doaj +1 more source
Regional distribution of the prostaglandin E2 receptor EP1 in the rat brain: accumulation in Purkinje cells of the cerebellum [PDF]
Prostaglandin E2 (PGE2), is a major prostanoid produced by the activity of cyclooxygenases (COX) in response to various physiological and pathological stimuli. PGE2 exerts its effects by activating four specific E-type prostanoid receptors (EP1, EP2, EP3
Akundi, Ravi S. +6 more
core
C2238/αANP modulates apolipoprotein E through Egr-1/miR199a in vascular smooth muscle cells in vitro [PDF]
Subjects carrying the T2238C ANP gene variant have a higher risk to suffer a stroke or myocardial infarction. The mechanisms through which T2238C/αANP exerts detrimental vascular effects need to be fully clarified.
Bianchi, Franca +9 more
core +1 more source
The use of anti-ribosomal P antibodies in the diagnosis of cerebral lupus: superiority of western blotting over enzyme-linnked immunosorbent assay [PDF]
This study investigated the use of anti-ribosomal P antibodies in the diagnosis of cerebral complications of systemic lupus erythematosus using two different methodsԸ?western blotting and enzyme-linked immunosorbent assay.
Chan, EYT +3 more
core
In vivo and ex vivo regulation of visfatin production by leptin in human and murine adipose tissue : role of mitogen-activated protein kinase and phosphatidylinositol 3-kinase signaling pathways [PDF]
Visfatin is an adipogenic adipokine with increased levels in obesity, properties common to leptin. Thus, leptin may modulate visfatin production in adipose tissue (AT).
Bee K. Tan +34 more
core +2 more sources
Western Multi-blotting Device - Improve the Productivity of Protein Transfer [PDF]
poster abstractDr. Stanley Chien from the Department of Electrical and Computer Engineering and Dr. Hiroki Yokota from Department of Biomedical Engineering jointly formed an interdisciplinary research team for the development of new devices to improve ...
Chien, Stanley, Yokota, Hiroki
core
CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +8 more
core +1 more source

