Results 71 to 80 of about 157,699 (364)
Nanoscale correlated disorder in out-of-equilibrium myelin ultrastructure [PDF]
ACS Nano 12(1), 729-739 (2018), 2017 Ultrastructural fluctuations at nanoscale are fundamental to assess properties and functionalities of advanced out-of-equilibrium materials. We have taken myelin as a model of supramolecular assembly in out-of-equilibrium living matter. Myelin sheath is a simple stable multi-lamellar structure of high relevance and impact in biomedicine. Although it is
arxiv +1 more source
The Anatomical Record, EarlyView.Abstract The trigeminus nerve (cranial nerve V) is a large and significant conduit of sensory information from the face to the brain, with its three branches extending over the head to innervate a wide variety of integumentary sensory receptors, primarily tactile.
Juri A. Miyamae +4 more
wiley +1 more source
Advances in methodologies for quantifying protein–protein interactions in living cells
Bulletin of the Korean Chemical Society, EarlyView.Measuring the binding affinities of protein–protein interactions (PPIs) is important for understanding underlying biological mechanisms. As methods for studying PPIs differ between in vitro and in‐cell conditions due to environmental differences, it is crucial to stay informed about recent trends and methodologies in both experimental contexts ...
Soojung Yi, Yejin Ahn, Nam Ki Lee
wiley +1 more source
Synuclein Analysis in Adult Xenopus laevis
International Journal of Molecular Sciences, 2022 The α-, β- and γ-synucleins are small soluble proteins expressed in the nervous system of mammals and evolutionary conserved in vertebrates. After being discovered in the cartilaginous fish Torpedo californica, synucleins have been sequenced in all vertebrates, showing differences in the number of genes and splicing isoforms in different taxa. Although
Maria Carmela Bonaccorsi di Patti +5 more
openaire +3 more sources
The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts
Cytoskeleton, EarlyView.ABSTRACT A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types.
Guadalupe E. Pena +4 more
wiley +1 more source
, 2003 Xenopus oocytes are a versatile expression system particularly suited for membrane transporters and channels. Oocytes have little background activity and therefore offer a very high signal-to-noise ratio for transporter and channel characterization. This chapter provides an overview of the basic methods used for the analysis of membrane transporters in
openaire +5 more sources
Dissecting the Structural Organization, Recruitment and Activation Mechanisms of Centrosomal γ‐TuRCs
Cytoskeleton, EarlyView.ABSTRACT Visualizing human centrosomes using cryo‐electron tomography revealed the native structure and molecular organization of γ‐tubulin ring complexes (γ‐TuRCs). γ‐TuRCs localized to two distinct centrosomal pools, one in the pericentriolar material (PCM) and another in the centriole lumen, which is released during mitosis.
Florian W. Hofer +5 more
wiley +1 more source
Notch activates Wnt-4 signalling to control medio-lateral patterning of the pronephros [PDF]
, 2009 Previous studies have highlighted a role for the Notch signalling pathway during pronephrogenesis in the amphibian Xenopus laevis, and in nephron development in the mammalian metanephros, yet a mechanism for this function remains elusive.
Jones, E. A., Naylor, Richard William
core +1 more source
Collective behavior of minus-ended motors in mitotic microtubule asters gliding towards DNA [PDF]
2014 Phys. Biol. 11 016008, 2014 Microtubules (MTs) nucleated by centrosomes form star-shaped structures referred to as asters. Aster motility and dynamics is vital for genome stability, cell division, polarization and differentiation. Asters move either towards the cell center or away from it.
arxiv +1 more source
Effective RNAi-mediated β2-microglobulin loss of function by transgenesis in Xenopus laevis
Biology Open, 2013 Summary To impair MHC class I (class I) function in vivo in the amphibian Xenopus, we developed an effective reverse genetic loss of function approach by combining I-SceI meganuclease-mediated transgenesis with RNAi technology.
Hristina Nedelkovska +3 more
doaj +1 more source